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Invisibleflavoraid
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brown rice flour mycelium culture
    #8045153 - 02/19/08 10:08 PM (15 years, 11 months ago)

I plan to prep 16cc water and 5 grams brown rice flour in a half pint jar. My goal is to fully colonize, agitate and make a bunch of inoculation syringes.

I'm not sure how I want to prep my jar lids , I want colonization as fast as possible without running much risk of contams. Thinking something like fahsters grain jar lids?

I'm also not sure exactly how long to steam / boil the jars for I'm thinking 30-45mins is fine. There isn't a lot of substance or depth to the jars theres like 21 grams of material in appose to 200+ in doc's tek he calls for 15mins pressure cooked at 15psi but I don't have a pressure cooker right now.

I'm using doc's tek as a guideline but I'm steaming instead of boiling, I want to possibly make a different type of lid and I'm using this to make syringes instead of a mycelium culture transfer like he used them for.

If anyone can tell me how well flooding a colonized PF cake and sucking up the mycelium rich liquid works as an inoculant that would be great too because I have a bunch of cakes that are fully colonized.


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coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.


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InvisibleRickster
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Re: brown rice flour mycelium culture [Re: flavoraid]
    #8045270 - 02/19/08 10:31 PM (15 years, 11 months ago)

I have seen posts with shroomerites doing something like this and results were just as good as using a lc. very quick results, forgot about it untill now... I believe I will do this soon! Thanks.


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Invisibleflavoraid
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Re: brown rice flour mycelium culture [Re: Rickster]
    #8045338 - 02/19/08 10:44 PM (15 years, 11 months ago)

http://www.shroomery.org/8514/Agar-substitute

theres a link to the tek by doc34

I dont have a pressure cooker and I think lids like fahster would be optimal. So I need to figure out my sterile procedure and I need to construct jar lids.

Using 1gram of BRF and 2.5-3ml of water would probably stomp I'm assuming it will fully colonize super super fast and it's still a HUGE amount of mycelium... considering small amounts go a long way. When I figure out my cooking and jar lids I'll make at least a couple that are 1gram brf to 2.5-3ml of water.


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coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.


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InvisibleRickster
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Re: brown rice flour mycelium culture [Re: Rickster]
    #8045376 - 02/19/08 10:50 PM (15 years, 11 months ago)

Here is somthing to consider, the pics have been deleted but it still has some great info.
https://www.shroomery.org/forums/showflat.php/Number/3462220#3462220


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Offlineresptodd
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Re: brown rice flour mycelium culture [Re: Rickster]
    #8045387 - 02/19/08 10:52 PM (15 years, 11 months ago)

I've tried some grain flour culture medias. It's very difficult to get right. Out of 20 jars, in sets of 4, using BRF. The 3rd set made it, for about 3 weeks. I used LC from a very strong master culture to 'noc all trials up. I don't know what happens, maybe an inability to break down the complex sugars in that type of environment, I'm not sure. But good luck, when it works (so I hear), it works well. I had laboratory grade filters on my lids, ran out of filters, so I gave up.


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Damn! I'm having fun! Just keep the GD monkeys away.


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Offlineresptodd
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Re: brown rice flour mycelium culture [Re: resptodd]
    #8045490 - 02/19/08 11:10 PM (15 years, 11 months ago)

Sorry, I hope haven't dissuaded you from trying. If you get it right, your jar will grow so thick with myc', it'll look like milk. That's why I tried--5 times. Again, Good Luck.


--------------------
Damn! I'm having fun! Just keep the GD monkeys away.


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Invisibleflavoraid
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Re: brown rice flour mycelium culture [Re: resptodd]
    #8046227 - 02/20/08 06:34 AM (15 years, 11 months ago)

Quote:

resptodd said:
I've tried some grain flour culture medias. It's very difficult to get right. Out of 20 jars, in sets of 4, using BRF. The 3rd set made it, for about 3 weeks. I used LC from a very strong master culture to 'noc all trials up. I don't know what happens, maybe an inability to break down the complex sugars in that type of environment, I'm not sure. But good luck, when it works (so I hear), it works well. I had laboratory grade filters on my lids, ran out of filters, so I gave up.




it should have been fully colonized by 3weeks no?

I just need healthy mycelium growth doesn't even have to be fully colonized as I just wanna make some inoculation syringes

besides this will cost me like a whole 30cents per experiment so I'm down. :laugh:


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coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.


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InvisiblePeterthinks
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Re: brown rice flour mycelium culture [Re: flavoraid]
    #8047323 - 02/20/08 01:30 PM (15 years, 11 months ago)

Myc just aren't picky.
you can even just shoot some sterile water into a half grown petri dish give it a swish with the needle and suck it back up.(should work with a cake too)
shoot into a new cake or into 4% water sugar LC solution...or both it doesn't take much at all to start LC just 2 drops will do it.
I've done the same thing with slants too.
experiment a bit with the brf water ratio...too much and it's runny too little and it dries into a little cake.
If you really want to do it in a jar I suggest you throw in half a marble or beer bottle shard so you can break up the mat easier.
I wouldn't bother waiting for it to fully grow over though.
:jester:


--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.


Edited by Peterthinks (02/20/08 01:33 PM)


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Invisibleflavoraid
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Re: brown rice flour mycelium culture [Re: Peterthinks]
    #8047472 - 02/20/08 02:07 PM (15 years, 11 months ago)

I have petri dishes but no pressure cooker atm.

agar is too hard to work with without a pressure cooker.

I was considering trying a malt/dextrose lc with boiling but I think this brf and water tek would be easier to achieve healthy mycelium?


--------------------
coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.


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Offlinemisos
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Re: brown rice flour mycelium culture [Re: flavoraid]
    #8047605 - 02/20/08 02:42 PM (15 years, 11 months ago)

How long do the BRF cultures last? Also, can you jsut start an LC with spores only? I'm new to this entire Mycology idea, and even newer to the LC stuff. Sorry, for jacking your thread BTW. :frown:


--------------------
"If I had a single wish, I would have every single human on this planet see this natural world the way I see it; the beauty in such simple things such as a fallen tree that is covered in moss and that has new trees growing from it. To some, fallen trees are ugly. But in reality, it is the circle of life at its finest. This is a beautiful world, its time that we recognize that before its all gone."


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Invisibleflavoraid
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Re: brown rice flour mycelium culture [Re: misos]
    #8047631 - 02/20/08 02:50 PM (15 years, 11 months ago)

you can start a liquid culture from spores.

spores to a source of water and nutrients such as 4% sugar (karo, honey, malt, dextrose) and water.

This is proven to work doc34 made a tek on it.


--------------------
coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.


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Offlinemisos
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Re: brown rice flour mycelium culture [Re: flavoraid]
    #8047682 - 02/20/08 03:01 PM (15 years, 11 months ago)

Know where I can find it? :confused: I suck at the search function.


--------------------
"If I had a single wish, I would have every single human on this planet see this natural world the way I see it; the beauty in such simple things such as a fallen tree that is covered in moss and that has new trees growing from it. To some, fallen trees are ugly. But in reality, it is the circle of life at its finest. This is a beautiful world, its time that we recognize that before its all gone."


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Invisibleflavoraid
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Re: brown rice flour mycelium culture [Re: misos]
    #8047711 - 02/20/08 03:07 PM (15 years, 11 months ago)

liquid culture teks... theres many

fahster does a master culture with grain that I really like but haven't tried.

http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6886717/an/0/page/0

http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6590986/an/0/page/1

fool proof-ish. but im lazy and dont wanna hunt down a cheap pressure cooker, want to try this brf tek instead.


--------------------
coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.


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Offlineresptodd
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Re: brown rice flour mycelium culture [Re: flavoraid]
    #8047758 - 02/20/08 03:16 PM (15 years, 11 months ago)

I would think a pressure cooker a must when working with grain flours. You have no idea of what endospores lurk within the flour.


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Damn! I'm having fun! Just keep the GD monkeys away.


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Offlinemisos
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Re: brown rice flour mycelium culture [Re: resptodd]
    #8047793 - 02/20/08 03:23 PM (15 years, 11 months ago)

Actually, just from my small amount of experience, I've done about 25 jars, little experience anyone, and so far none of them have contaminated. I have never pressure cooked them, up until today. I had contam and bacteria problems with corn and rye. I must just suck.:smirk:
Eh. I get my product.


--------------------
"If I had a single wish, I would have every single human on this planet see this natural world the way I see it; the beauty in such simple things such as a fallen tree that is covered in moss and that has new trees growing from it. To some, fallen trees are ugly. But in reality, it is the circle of life at its finest. This is a beautiful world, its time that we recognize that before its all gone."


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Invisibleflavoraid
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Re: brown rice flour mycelium culture [Re: resptodd]
    #8047813 - 02/20/08 03:29 PM (15 years, 11 months ago)

rice doesn't have endospores from what I've read, I just dont know how long to cook for.

I steam my cakes for 2hours and they come out fine.

I could even nuke it in the microwave for a while or something.


--------------------
coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.


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InvisiblePeterthinks
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Re: brown rice flour mycelium culture [Re: flavoraid]
    #8048741 - 02/20/08 07:18 PM (15 years, 11 months ago)

You can't go very far without a PC.

I got 3 :wink:
You gotta soak corn and rye so the contams crawl out of their little cysts and you can kill em.
:jester:


--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.


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Invisibleflavoraid
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Re: brown rice flour mycelium culture [Re: Peterthinks]
    #8048817 - 02/20/08 07:34 PM (15 years, 11 months ago)

man what the fuck.

I've been asking about BROWN RICE as in the shit you make PF cakes with as in easily without a pressure cooker.

All I get is questions or replies that aren't related to brown rice at all.


--------------------
coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.


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InvisiblePeterthinks
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Registered: 11/10/04
Posts: 2,379
Re: brown rice flour mycelium culture [Re: flavoraid]
    #8048891 - 02/20/08 07:47 PM (15 years, 11 months ago)

Quote:

flavoraid said:
man what the fuck.

I've been asking about BROWN RICE as in the shit you make PF cakes with as in easily without a pressure cooker.

All I get is questions or replies that aren't related to brown rice at all.



Why yes I do think professional wrestling is fixed...But why would you bring that up in a thread about Jessica Alba?
:jester:


--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.


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Offlineresptodd
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Re: brown rice flour mycelium culture [Re: Peterthinks]
    #8049128 - 02/20/08 08:42 PM (15 years, 11 months ago)

What do you want to hear?


--------------------
Damn! I'm having fun! Just keep the GD monkeys away.


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