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gigs
Stranger
Registered: 02/06/08
Posts: 6
Last seen: 15 years, 11 months
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cold shock ?
#8042289 - 02/19/08 10:43 AM (16 years, 1 month ago) |
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Hey guys, I 'm growing ecuadorian shrooms with a spore print, in jars of rice ( pf tek ), and i have inoculated 3 weeks ago, i had 2 jars contamined with green mold ( with good mycelium in 3 days, anyway i tossed them ), so i have just one jar. This jar is a little white-grey,and somewhere a little more white, maybe the beginning of colonisation, i don't know, but it doesn't seem to evoluate. The T°C are ok ( 28°C ). I ve wonder if a cold shock can help to activate colonisation ? if yes, how do this ? fridge or freezer ? 12-24 hr ? or others ? Thank you.
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Rickster
El Conquistador
Registered: 02/06/06
Posts: 659
Loc: Babylon
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Re: cold shock ? [Re: gigs]
#8042312 - 02/19/08 10:50 AM (16 years, 1 month ago) |
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I have heard cold shock doesn't work for tropical mushrooms, usually it is done to fruit your cakes, not for colonization.
Go to search "cold Shock". But for now I did a search for you here is a link! https://www.shroomery.org/forums/search.php
Edited by Rickster (02/19/08 10:57 AM)
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Slimz
.-~*´`*·~-experience-~*´`*·~-.
Registered: 10/03/07
Posts: 3,588
Loc: Maryland
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Re: cold shock ? [Re: Rickster]
#8042345 - 02/19/08 10:57 AM (16 years, 1 month ago) |
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cold shock is for cold weather shrooms like shitake, not cubes..
-------------------- Lazy Drywall Tek (no powdery mess) This series will blow your mind and confirm what you already know to be true. The Pharmacratic Inquisition Best Thread Ever ! ! ! me if you have questions about lasers Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles. FeelFamily resident tech guru
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Rickster
El Conquistador
Registered: 02/06/06
Posts: 659
Loc: Babylon
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Re: cold shock ? [Re: Rickster]
#8042362 - 02/19/08 11:00 AM (16 years, 1 month ago) |
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dumbfounded1600
Stranger
Registered: 07/29/07
Posts: 2,624
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Re: cold shock ? [Re: Rickster]
#8042391 - 02/19/08 11:07 AM (16 years, 1 month ago) |
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You dunk in the refrigerator to prevent bacteria buildup that would otherwise contaminate your brf if you dunked at room temperature. Don't confuse that with cold shocking, although it does cold shock them...lol As for cased bulk substrates, I ran several experiments a few years ago where half the trays were placed in the refrigerator for 24 hours, while the second half were left at room temperature. Both sets of trays went to the fruiting chamber at the same time. Every single tray that had been 'cold shocked' pinned two or more days later than trays that had not been cold shocked. That is not to say a temperature change can't be a pinning trigger. However, along with light, a fully colonized substrate, and an increase in air exchange, it is only one of the triggers. However, cold shock being beneficial or not, you need to dunk your cakes in the refrigerator. RR
Cold shocking doesn't benefit from cubes because cubes are a tropical species, not cold weather.
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 29 days
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If it's not colonized by now, it's contaminated. Toss it and start over. 28 is near the upper end for performance, so I'd drop it to the 25C-26C range for the next batch.
Cold shock is a term that needs to be removed from the vocabulary of those growing tropical species. Cold shocking is for fall fruiting edible and medicinal mushrooms such as shiitake. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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thashaman
Biophilosopher
Registered: 11/09/04
Posts: 161
Last seen: 14 years, 15 days
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Re: cold shock ? [Re: gigs]
#8042600 - 02/19/08 12:02 PM (16 years, 1 month ago) |
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Yea, RR said it right..."Cold Shocking" can also be used albeit incorrectly, to describe the process whereby PF style cakes are dunked for no less than 12 hours and preferably 24 hours in the refrigerator to induce fruiting. It has worked for me in the past for PESA strain and Hawaiian quite well.
As mushrooms get ready to fruit, there are three things that signal this phase of life. Light, about a 10-15 degree temperature drop, and air exchange. The mycelial growth phase i.e. colonization tends to occur optimally at slightly higher temperatures (76-80 F.) and higher humidity. For fruiting you will want to drop the temperature about 6-8 degrees, and decrease humidity from 100% to around 90%...or even less if you are using a casing because casings provide their own endogenous humidification. Just curious though, are you utilizing an incubator-Tek during colonization?
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gigs
Stranger
Registered: 02/06/08
Posts: 6
Last seen: 15 years, 11 months
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Re: cold shock ? [Re: thashaman]
#8042781 - 02/19/08 12:59 PM (16 years, 1 month ago) |
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ok, i m gonna wait a little and toss it if always nothing... thanks hope the next will ba alright
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