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Offlinegigs
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cold shock ?
    #8042289 - 02/19/08 10:43 AM (15 years, 11 months ago)

Hey guys,
I 'm growing ecuadorian shrooms with a spore print, in jars of rice ( pf tek ), and i have inoculated 3 weeks ago, i had 2 jars contamined with green mold ( with good mycelium in 3 days, anyway i tossed them ), so i have just one jar.
This jar is a little white-grey,and somewhere a little more white, maybe the beginning of colonisation, i don't know, but it doesn't seem to evoluate.
The T°C are ok ( 28°C ).
I ve wonder if a cold shock can help to activate colonisation ? if yes, how do this ? fridge or freezer ? 12-24 hr ? or others ?
Thank you.


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InvisibleRickster
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Re: cold shock ? [Re: gigs]
    #8042312 - 02/19/08 10:50 AM (15 years, 11 months ago)

I have heard cold shock doesn't work for tropical mushrooms, usually it is done to fruit your cakes, not for colonization.

Go to search "cold Shock". But for now I did a search for you here is a link! https://www.shroomery.org/forums/search.php


Edited by Rickster (02/19/08 10:57 AM)


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InvisibleSlimz
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Re: cold shock ? [Re: Rickster]
    #8042345 - 02/19/08 10:57 AM (15 years, 11 months ago)

cold shock is for cold weather shrooms like shitake, not cubes..


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InvisibleRickster
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Re: cold shock ? [Re: Rickster]
    #8042362 - 02/19/08 11:00 AM (15 years, 11 months ago)



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Invisibledumbfounded1600
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Re: cold shock ? [Re: Rickster]
    #8042391 - 02/19/08 11:07 AM (15 years, 11 months ago)

You dunk in the refrigerator to prevent bacteria buildup that would otherwise contaminate your brf if you dunked at room temperature. Don't confuse that with cold shocking, although it does cold shock them...lol
As for cased bulk substrates, I ran several experiments a few years ago where half the trays were placed in the refrigerator for 24 hours, while the second half were left at room temperature. Both sets of trays went to the fruiting chamber at the same time. Every single tray that had been 'cold shocked' pinned two or more days later than trays that had not been cold shocked.
That is not to say a temperature change can't be a pinning trigger. However, along with light, a fully colonized substrate, and an increase in air exchange, it is only one of the triggers. However, cold shock being beneficial or not, you need to dunk your cakes in the refrigerator.
RR


Cold shocking doesn't benefit from cubes because cubes are a tropical species, not cold weather.


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OfflineRogerRabbitM
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Re: cold shock ? [Re: dumbfounded1600]
    #8042515 - 02/19/08 11:44 AM (15 years, 11 months ago)

If it's not colonized by now, it's contaminated. Toss it and start over. 28 is near the upper end for performance, so I'd drop it to the 25C-26C range for the next batch.

Cold shock is a term that needs to be removed from the vocabulary of those growing tropical species. Cold shocking is for fall fruiting edible and medicinal mushrooms such as shiitake.
RR


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Offlinethashaman
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Re: cold shock ? [Re: gigs]
    #8042600 - 02/19/08 12:02 PM (15 years, 11 months ago)

Yea, RR said it right..."Cold Shocking" can also be used albeit incorrectly, to describe the process whereby PF style cakes are dunked for no less than 12 hours and preferably 24 hours in the refrigerator to induce fruiting. It has worked for me in the past for PESA strain and Hawaiian quite well.

As mushrooms get ready to fruit, there are three things that signal this phase of life. Light, about a 10-15 degree temperature drop, and air exchange. The mycelial growth phase i.e. colonization tends to occur optimally at slightly higher temperatures (76-80 F.) and higher humidity. For fruiting you will want to drop the temperature about 6-8 degrees, and decrease humidity from 100% to around 90%...or even less if you are using a casing because casings provide their own endogenous humidification. Just curious though, are you utilizing an incubator-Tek during colonization?


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Offlinegigs
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Re: cold shock ? [Re: thashaman]
    #8042781 - 02/19/08 12:59 PM (15 years, 11 months ago)

ok, i m gonna wait a little and toss it if always nothing...
thanks
hope the next will ba alright


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