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NinjaZX6R
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Registered: 02/15/08
Posts: 52
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BRF jars myc growth
#8037341 - 02/18/08 10:38 AM (15 years, 11 months ago) |
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Ok, so I knocked up about 35-40 jars total, 6 on 2/4 and the rest from 2/14 up until 2/17. Im a little worried because of all of those jars only one has myc growing (inoculated 2/4). I posted about a week ago questioning if my jars might be too try but couldnt get any pics. I decided to open one of my jars from 2/4 to see if the substrate might be too dry or too wet. So I opened it and it was moist to the touch, not overly moist but just enough to tell basically. I then threw this jar out. I used the same tek for all of my jars and used syringes from two different vendors.
This is the best pic I could get with what my jars look like. This is the one with some growth.

Please let me know if I am doing something wrong!
BTW, boiled 90 mins, used 2 cups verm, 1 cup brf, and 1 cup water. This was what to do according to the video I bought.
I appreciate your input!
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
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Re: BRF jars myc growth [Re: NinjaZX6R]
#8037368 - 02/18/08 10:49 AM (15 years, 11 months ago) |
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what is on those jars for means of gas exchange?
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NinjaZX6R
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Re: BRF jars myc growth [Re: blood4blood]
#8037371 - 02/18/08 10:50 AM (15 years, 11 months ago) |
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Actually just recently took tinfoil off of the ones knocked up on 2/4. The holes in the top are now open to get some air flow.
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NinjaZX6R
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Re: BRF jars myc growth [Re: NinjaZX6R]
#8037378 - 02/18/08 10:52 AM (15 years, 11 months ago) |
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Sorry the 2 cups verm, 1 cup BRF, and 1cup H2O are for every 5 jars.
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Fahkface
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Re: BRF jars myc growth [Re: NinjaZX6R]
#8037381 - 02/18/08 10:53 AM (15 years, 11 months ago) |
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First of all: Welcome!
As you might know by now, it's a pretty bad idea to knock up 40 jars without any experience. You should have maybe knocked up 5 or 6 for the beginning.
Even the jar the myc grows in looks too moist, so might want to try it again, with less water.
I never did it the way RR described in the video, but just because I started off with another instruction and never changed.
I use to to fill five jars (that's the number that fits in my PC), to 3/4 with Verm, than put in a big bowl and add water to the point it rinses slightly when you hold it a angle of about 45 degrees. You shouldn't see too much water, just a little bit!!! The superfluous water will be soaked by the BRF that should be added AFTER you added the water to the verm, so you can see the right degree of water content better.
The rest is the same procedure.
If you've done it a few times, you should be fine. I think this version is a little more precise, because different grades of verm soak off different amounts of water (fine graded verm is too wet, when you see as much water in the bowl, as you'd see, when using middle graded verm).
This might sound complicated, but it isn't after you've done it a few times.
Anyway, for the next time, use LESS jars and try to be a little more precise! Wait a few more days for your current jars to be thrown out. Maybe something will show up after all.
As soon as they start to stink, throw them out!
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SLAG
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Re: BRF jars myc growth [Re: NinjaZX6R]
#8037390 - 02/18/08 10:56 AM (15 years, 11 months ago) |
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gas exchange is very important for incubation---LEAVE THE FOIL AND TAPE OR WHATEVER THE HELL YOU HAVE ON THERE OFF>big difference in speed of colonization or colonization period. If they go more than two weeks with no growth...something went terribly wrong.
-------------------- I post lies to look cool
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blood4blood
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Registered: 04/25/07
Posts: 6,029
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Re: BRF jars myc growth [Re: NinjaZX6R]
#8037394 - 02/18/08 10:56 AM (15 years, 11 months ago) |
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you have the top protective verm layer then? if so thats probabally why they are slowed down because you had tha foil on them. these are what my jars look like after just 4 days.
Edited by blood4blood (02/18/08 11:00 AM)
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Fahkface
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Re: BRF jars myc growth [Re: Fahkface]
#8037396 - 02/18/08 10:57 AM (15 years, 11 months ago) |
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When you use tin foil as a lid, you don't have to worry about the air exchange! Since the tin foil isn't too tight, there will be enough gas exchange!
I do it all the time (actually never used anything else) and never had any bigger problems with it.
Taking off the tin foil will probably just result in contams, but since your jars seem to be ruined anyway, I guess it doesn't matter
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NinjaZX6R
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Re: BRF jars myc growth [Re: Fahkface]
#8037479 - 02/18/08 11:18 AM (15 years, 11 months ago) |
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I do have the verm on the top layer. I know I shouldnt have done so many, but I was so damn frustrated after only one of my jars from 2/4 showed growth I went all out =). Blood, what is the tek you used for yours? I used the RR vid. Also, been keeping these at jars at about 75-80F. I am figuring with my first jars I might have inoculated them too quickly, as for th newer ones....... I think my first batch has me really paranoid. I do still have a syring and a half left to use, so I think I am going to attempt using a different tek.
When miing the verm and water I did see a little puddle at the bottom, then threw in the brf and mixed very well. I am hoping nothing is wrong and its just parnoia. Damn, this can be frustrating!
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blood4blood
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Re: BRF jars myc growth [Re: NinjaZX6R]
#8037502 - 02/18/08 11:27 AM (15 years, 11 months ago) |
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i used that same ratio. 2:1:1. thats a good ratio and it always works for me. id say it has alot to do with keeping that foil on the tops of those. on top of having the verm layer and the metal lid that will slow your growth down. i dont think your cakes are doomed. just stop peeking on them and check on them in a week with the foli off and i bet youll have some nice amounts of growth.
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Fahkface
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Re: BRF jars myc growth [Re: blood4blood]
#8037657 - 02/18/08 12:07 PM (15 years, 11 months ago) |
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Well, i can't tell if the jars are doomed or not and I can't tell if not using tin foil is better, but usually my jars colonize in about 10 to 13 days (depending on how old the spores I use are) and from I've read and hear it's average.
I don't have any lids to try it, so maybe someone should should try a little competition, using a maybe three jars with a "professional" lid and three with tin foil, also using the same spawn and the same syringe to inoculate.
Actually I'm quiet happy with my results, so I'm in no need of any faster colonization but it would be interesting to see what results it brings.
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blood4blood
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Re: BRF jars myc growth [Re: Fahkface]
#8037671 - 02/18/08 12:10 PM (15 years, 11 months ago) |
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im sure just tinfoil would colonize as fast as the metal lid with the 4 holes. but he had both. maybe ill try today, im about to make up anther batch of brf cakes.
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Fahkface
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Re: BRF jars myc growth [Re: blood4blood]
#8037680 - 02/18/08 12:12 PM (15 years, 11 months ago) |
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Ah, OK, I didn't see it. Well, I have no idea if this effects the growth. I've never done that
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tahoe
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Re: BRF jars myc growth [Re: blood4blood]
#8037690 - 02/18/08 12:15 PM (15 years, 11 months ago) |
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the foil is okay left on. It does not form a seal of any type. More impartant is the 1/2 verm layer. Also your jars suck!!! You are supposed to use 1/2pint wide mouth. These are used for a reason. Also that shitty ass criss cross design on the jar is horrible for pictures. Your jars look super wet it could just be the jars. Your pot should not have more than an 1/2 inch of water in it
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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Serial
Cake or Death?




Registered: 11/10/07
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Re: BRF jars myc growth [Re: tahoe]
#8037754 - 02/18/08 12:35 PM (15 years, 11 months ago) |
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I wouldn't worry, it took me 20 days to start seeing mycelium using that same ratio. This was due to low gas exchange because I left the foil on too long. You'll be fine. Incubate to speed up the process.
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blood4blood
Calmer Than You Are


Registered: 04/25/07
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Re: BRF jars myc growth [Re: Serial]
#8037820 - 02/18/08 12:49 PM (15 years, 11 months ago) |
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Quote:
Serial said: Incubate to speed up the process.
this will also give you a higher risk of contamination. room temp is fine for colonizing
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tahoe
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Re: BRF jars myc growth [Re: blood4blood]
#8037837 - 02/18/08 12:53 PM (15 years, 11 months ago) |
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yeah and a higher risk of doing something stupid. Just grow at room temp until you have the faintest idea of what you are doing
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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NinjaZX6R
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Re: BRF jars myc growth [Re: tahoe]
#8037885 - 02/18/08 01:04 PM (15 years, 11 months ago) |
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These are 1/2 pint wide mouth, and I know the reason they're used. As for the diamond shaped pattern, sorry, didnt have a choice man, only this was available. Ill try to get pics of my other jars that dont have the pattern, I was worried they were too dry, not too wet. I can see very little moisture on the side of the glass. Trust me I followed the tek to a T! Watched the vid like a hundred times before and while I was making them.
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tahoe
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Re: BRF jars myc growth [Re: NinjaZX6R]
#8037908 - 02/18/08 01:10 PM (15 years, 11 months ago) |
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I do not think so. A half pint wide mouth jar could only be so tall. You either have(which I believe) 1/2 pint regular mouth or 1 pint wide mouth. Most likely the 1/2 pint small mouth.

These are 1/2 pint wide mouth.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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blood4blood
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Registered: 04/25/07
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Re: BRF jars myc growth [Re: tahoe]
#8037923 - 02/18/08 01:13 PM (15 years, 11 months ago) |
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stealing my fucing pics tahoe!
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Serial
Cake or Death?




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Re: BRF jars myc growth [Re: blood4blood]
#8037997 - 02/18/08 01:30 PM (15 years, 11 months ago) |
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Quote:
blood4blood said:
this will also give you a higher risk of contamination. room temp is fine for colonizing
I disagree, if his technique was sloppy and there's contaminants in the jars already I fail to see how a low 80's incubation temp has anything to do with increasing the risk of contamination.
His question is not about his sterile technique anyway, its colonization time, which was my problem my first time out also. I incubated at 82F and it sped the process up quite a bit from the 72F my room was at prior.
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blood4blood
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Re: BRF jars myc growth [Re: Serial]
#8038042 - 02/18/08 01:40 PM (15 years, 11 months ago) |
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higher temps are where mold spores love to thrive and germinate
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NinjaZX6R
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Re: BRF jars myc growth [Re: blood4blood]
#8038057 - 02/18/08 01:42 PM (15 years, 11 months ago) |
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Whatever it is, works the same. If a cake needs to come out in one solid piece it will with these. Mouth is no smaller than the jar itself. That is the purpose, right? Please correct me if Im wrong. Would suck to be thinking one thing this whole time and turn out to be wrong bout it.
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Fahkface
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Re: BRF jars myc growth [Re: Serial]
#8038078 - 02/18/08 01:44 PM (15 years, 11 months ago) |
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Molds and bacteria love moist and warm environments, therefore increasing the temperature, leads to better circumstances for all kinds of organisms (such as Ps. cubensis OR molds). We introduce a whole army of cube spores to a wannabe sterile environment and it's pretty likely that the cube spores will win the fight for territory i.e. the spawn, because of their amount. However at the same time, as our cubes spores grow, molds and bacteria grow and the only reason for the jar not be fucked is the fact that the cube spores munch through the spawn and preventing it from being conquered by bacteria or molds. While mushroom mycelium can grow quiet well at a rather "low" temperature, bacteria and molds feel better the warmer it gets. The higher the temps, the better the bacteria and mold spores "feel" and grow faster. So does our cube mycelium, but it doesn't speed up as much as other organisms. Therefore it's likely to let your jars colonize at room temp. The mushroom spores simply have a slight advantage to the rest of the fellas, stuck in a jar!
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shevanel
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Re: BRF jars myc growth [Re: blood4blood]
#8038083 - 02/18/08 01:45 PM (15 years, 11 months ago) |
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Quote:
blood4blood said: stealing my fucing pics tahoe!
too funny... but at least tahoe takes the time to not only say what's right but show too..
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tahoe
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Re: BRF jars myc growth [Re: shevanel]
#8038167 - 02/18/08 02:04 PM (15 years, 11 months ago) |
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btw, the verm layer on top of the jar is intened to work while the jar is standing up right. You guys get your jars and start rolling them around and the verm comes loose and in comes the contams. Stop fucking with your jars. Do not turn them sideways to get a pic. If the jar is done correctly then you can do this because the verm will not roll around but if you had done them correctly you would not be posting here
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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NinjaZX6R
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Re: BRF jars myc growth [Re: tahoe]
#8038205 - 02/18/08 02:14 PM (15 years, 11 months ago) |
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Tahoe, I definately appreciate your insight and information as to what may be wrong, but man I think there might be other ways to word what you think. Dont get me wrong, I do appreciate evrything but its not necessary to talk down to me because Im new to this.
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blood4blood
Calmer Than You Are


Registered: 04/25/07
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Re: BRF jars myc growth [Re: NinjaZX6R]
#8038233 - 02/18/08 02:23 PM (15 years, 11 months ago) |
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you'll just have to get used to tahoe brother. you'll just have to show him you know what your talking about and dont do dumb shit and prove that you belong here and he'll leave ya alone.
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