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OfflineSerial
Cake or Death?
Male


Folding@home Statistics
Registered: 11/10/07
Posts: 58
Loc: Middle of Nowhere
Last seen: 8 years, 11 months
Re: BRF jars myc growth [Re: blood4blood]
    #8037997 - 02/18/08 01:30 PM (15 years, 11 months ago)

Quote:

blood4blood said:

this will also give you a higher risk of contamination. room temp is fine for colonizing




I disagree, if his technique was sloppy and there's contaminants in the jars already I fail to see how a low 80's incubation temp has anything to do with increasing the risk of contamination.

His question is not about his sterile technique anyway, its colonization time, which was my problem my first time out also. I incubated at 82F and it sped the process up quite a bit from the 72F my room was at prior.


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Invisibleblood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: BRF jars myc growth [Re: Serial]
    #8038042 - 02/18/08 01:40 PM (15 years, 11 months ago)

higher temps are where mold spores love to thrive and germinate


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OfflineNinjaZX6R
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Registered: 02/15/08
Posts: 52
Last seen: 15 years, 8 months
Re: BRF jars myc growth [Re: blood4blood]
    #8038057 - 02/18/08 01:42 PM (15 years, 11 months ago)

Whatever it is, works the same. If a cake needs to come out in one solid piece it will with these. Mouth is no smaller than the jar itself. That is the purpose, right? Please correct me if Im wrong. Would suck to be thinking one thing this whole time and turn out to be wrong bout it.


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OfflineFahkface
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Registered: 12/11/06
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Re: BRF jars myc growth [Re: Serial]
    #8038078 - 02/18/08 01:44 PM (15 years, 11 months ago)

Molds and bacteria love moist and warm environments, therefore increasing the temperature, leads to better circumstances for all kinds of organisms (such as Ps. cubensis OR molds).
We introduce a whole army of cube spores to a wannabe sterile environment and it's pretty likely that the cube spores will win the fight for territory i.e. the spawn, because of their amount.
However at the same time, as our cubes spores grow, molds and bacteria grow and the only reason for the jar not be fucked is the fact that the cube spores munch through the spawn and preventing it from being conquered by bacteria or molds.
While mushroom mycelium can grow quiet well at a rather "low" temperature, bacteria and molds feel better the warmer it gets.
The higher the temps, the better the bacteria and mold spores "feel" and grow faster. So does our cube mycelium, but it doesn't speed up as much as other organisms.
Therefore it's likely to let your jars colonize at room temp.
The mushroom spores simply have a slight advantage to the rest of the fellas, stuck in a jar!


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Offlineshevanel
Gone til November
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Registered: 01/27/08
Posts: 1,517
Last seen: 1 year, 11 months
Re: BRF jars myc growth [Re: blood4blood]
    #8038083 - 02/18/08 01:45 PM (15 years, 11 months ago)

Quote:

blood4blood said:
stealing my fucing pics tahoe!




too funny... but at least tahoe takes the time to not only say what's right but show too..


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Invisibletahoe
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Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: BRF jars myc growth [Re: shevanel]
    #8038167 - 02/18/08 02:04 PM (15 years, 11 months ago)

btw, the verm layer on top of the jar is intened to work while the jar is standing up right. You guys get your jars and start rolling them around and the verm comes loose and in comes the contams. Stop fucking with your jars. Do not turn them sideways to get a pic.
If the jar is done correctly then you can do this because the verm will not roll around but if you had done them correctly you would not be posting here


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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OfflineNinjaZX6R
Noob
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Registered: 02/15/08
Posts: 52
Last seen: 15 years, 8 months
Re: BRF jars myc growth [Re: tahoe]
    #8038205 - 02/18/08 02:14 PM (15 years, 11 months ago)

Tahoe, I definately appreciate your insight and information as to what may be wrong, but man I think there might be other ways to word what you think. Dont get me wrong, I do appreciate evrything but its not necessary to talk down to me because Im new to this.


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Invisibleblood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: BRF jars myc growth [Re: NinjaZX6R]
    #8038233 - 02/18/08 02:23 PM (15 years, 11 months ago)

you'll just have to get used to tahoe brother. you'll just have to show him you know what your talking about and dont do dumb shit and prove that you belong here and he'll leave ya alone.


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