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hottamali
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Registered: 02/05/08
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Growing Sclerotia In-vitro
#8034614 - 02/17/08 12:53 PM (15 years, 11 months ago) |
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Does this sound right.
My friend is using pint jars, when he is mixing the rye grass seed he will use a 2:1 recipe of 2 parts rye grass seed with 1 part half drinking strength coffee in place of the water (coffee to keep seeds from germinating). He will add a pinch of gypsum which he will crush from a piece of Sheetrock from Home Depot (not sure if this is the same as horticulture gypsum or if he needs to get horticulture gypsum )
Fill the jars 1/2 to 2/3 full and then let them sit for a few hours and then pressure cook.
Inoculate with about 2 cc's of either P. tampanensis or Atlantis and then DO NOT shake jars at this time it will slow things down since using spores(as stated by Roger Rabbit). Store in dark area with a Temp in the lower 70's. However my friend says that his indoor temp is stable year round at around 78 - 80. (Could this be a problem?)
He can Shake the jars once or twice after mycelium is at 20% and maybe 75% but not after sclerotia starts to form. (not real sure about when to shake at this point)
What do you think? Is there anything you would change or suggest be done differently?
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Rose
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Re: Growing Sclerotia In-vitro [Re: hottamali]
#8034667 - 02/17/08 01:06 PM (15 years, 11 months ago) |
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Wow... you are missing a HUGE step.
Pressure cooking.
Unless you cook the jars, you will fail.
I don't know that you NEED gypsum or coffee... coffee will NOT replace STERILIZATION.
You CAN shake your jars, if they are properly filtered. 2 cc is enough spores to shake the jar and spread 'em around. Be sure to inject along the glass and not straight into the substrate.
High 70's should be fine.
-------------------- Fiddlesticks.
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hottamali
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Re: Growing Sclerotia In-vitro [Re: Rose]
#8034710 - 02/17/08 01:20 PM (15 years, 11 months ago) |
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Cervantes, thanks I've been reading a lot of your posts and got some good info on sclerotia for my friend. I know he has to PC I stated that in my post. How does the rest look. I got the coffee and gypsum input from RR post's
Edited by hottamali (02/17/08 01:21 PM)
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Rose
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Re: Growing Sclerotia In-vitro [Re: hottamali]
#8034736 - 02/17/08 01:29 PM (15 years, 11 months ago) |
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Hahaha... I missed when you said pressure cook... and I read that post TWICE! Sorry.
Gypsum will keep it from clumping... assuming you do it correctly. Without it, the grass seed WILL stick... it is a sticky, STICKY grain. But mexicanae is fine on clumpy substrate (since it takes a couple months before you harvest, it WILL be fully colonized by the time you open it)... it is just harder to shake effectively.
I think mexicanae myc is SO pretty, I'd have trouble shaking the jar after I saw growth. My process would be slower... but the jars would look great!
Coffee will speed growth... again assuming you do it correctly.
But both steps COULD complicate things. They shouldn't but they might. I just say this because, when you're new to this hobby, it is the extra steps that cause mistakes.
Do 'em if you want... but know you don't need gypsum and coffee. Water and rye grass seed will work fine.
I have heard good things about coffee and Mexicanae.
Good luck.
-------------------- Fiddlesticks.
Edited by Rose (02/17/08 01:38 PM)
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freetree77
king louie



Registered: 12/09/06
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Re: Growing Sclerotia In-vitro [Re: Rose]
#8034739 - 02/17/08 01:29 PM (15 years, 11 months ago) |
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no, he did mention pressure cooking, right after filling them 2/3 full.
i dont know about the sheetrock, but everything else sounds ok. 78-80 degrees is fine. not optimum, but it will work fine. ive been looking into growing in vitro my self since me ma and i are about to move back in together, i might even try to find a good mixture with some hpoo. let me know how well it turns out for you.
peace
whoops. guess i came in a little late
-------------------- Sinister Minister
Edited by freetree77 (02/17/08 01:39 PM)
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hottamali
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Re: Growing Sclerotia In-vitro [Re: Rose]
#8034785 - 02/17/08 01:43 PM (15 years, 11 months ago) |
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He will try them with and without the gypsum and coffee. He is going to try Atlantis and P. Tampanensis. The supplier did not have the mexicanae. He is pretty anal retentive so I think he will be extra careful with the extra steps. He has some cakes that are looking good. So far on his first try 10 for 10 but they ars still only 15 days old and a lot of bad can still happen. I think he is more excited about growing stones. Everything you read is real positive on them.
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Cheesekiller
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Re: Growing Sclerotia In-vitro [Re: hottamali]
#8034807 - 02/17/08 01:48 PM (15 years, 11 months ago) |
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Just make sure to shake your jars as soon as you can after sterilizing. This mistake has F'd me before, you cant shake them at all if you miss this step. But if you do it, then when it comes time to shake your myc up it will be easy, and it will ultimately be easy to get the spawn out of your jars when you are transfering it to your bulk.
-CK
-------------------- Bulk grower with "some" success. Cloning Machine Nice Lids A few pics of my DT setup
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Rose
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Re: Growing Sclerotia In-vitro [Re: hottamali]
#8034809 - 02/17/08 01:50 PM (15 years, 11 months ago) |
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If you can grow cakes, and have a PC... you can do Mexicanae. Good luck.
-------------------- Fiddlesticks.
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hottamali
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Re: Growing Sclerotia In-vitro [Re: Cheesekiller]
#8034817 - 02/17/08 01:53 PM (15 years, 11 months ago) |
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Not planning to spawn or transport. Just want stones. I beleive that the gypsum prevents the clumping so that you can shake later. But again I've read a few posts that do not recommend shaking right after inoculation if using spores.
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freetree77
king louie



Registered: 12/09/06
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Re: Growing Sclerotia In-vitro [Re: hottamali]
#8034837 - 02/17/08 02:01 PM (15 years, 11 months ago) |
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cheesekiller was saying you want to shake them immediatley after pcing before they even cool to prevent the grain from sticking together. as for shaking the jars after inoculation thats your decision. personally i would just wait til i see some good colonization before shaking.
-------------------- Sinister Minister
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hottamali
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Re: Growing Sclerotia In-vitro [Re: freetree77]
#8034840 - 02/17/08 02:02 PM (15 years, 11 months ago) |
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I missed that step, thanks
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hottamali
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Re: Growing Sclerotia In-vitro [Re: freetree77]
#8034850 - 02/17/08 02:05 PM (15 years, 11 months ago) |
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1
Edited by hottamali (02/17/08 02:06 PM)
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