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Smushroom
Avid Learner
Registered: 02/02/05
Posts: 2,806
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Fastest Method to Produce A Shroom
#8030209 - 02/16/08 05:40 AM (15 years, 11 months ago) |
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Now before you flame me let me explain what I am asking. I am starting agar work soon and I have a pretty hefty project in mind but I would really like to fruit each and every substrain I isolate in the process. I don't want to fruit them all bulk because I don't really need that many and I don't have the time to do a large PF grow with normal 1/2 pints.
I am wondering if anyone has experimented with various ways to produce fruits ASAP. I don't care about quantity, a single fruit would be enough to test various traits I would like to isolate.
I have been thinking about 2 options:
1) Fruit directly off of agar. Just have a single plate for each isolate strain. Put them all in a FC and see if they pin. Not sure how well that would work though.
2) The other option is to do a mini-PF Tek grow. I was thinking about using shot glasses. I would imagine with a LC of a single isolate strain it would colonize fully in 3 days, 3 more to pin, 4-5 to get to maturity, maybe less. That means under 2 weeks start to finish. I figure that is the fastest I could ask for.
Anyone else ever tried anything of this nature?
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HybridprX
Biodegrader of coir



Registered: 01/29/08 
Posts: 2,588
Loc: Canada
Last seen: 6 years, 4 months
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Re: Fastest Method to Produce A Shroom [Re: Smushroom]
#8030253 - 02/16/08 06:30 AM (15 years, 11 months ago) |
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Once the agar is colonized just give it 12 on 12 off of light a day, Ive accidentally had them pin on me while still in the jars from introducing a fully run plate to light to much.
You could just leave a open plate fruiting at room temp, that will work to but you need to be carful that the mycelium does not dry out, so a PmP "Poor mans pod" is advisable, just cutt a two liter pop bottle in half and slide the two sections together, place a damp paper towel in the bottom to help use that moisture to humidify the container rather then have it drawn away from the agar plate itself.
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Hawaiian
mycophile



Registered: 01/17/08
Posts: 57
Last seen: 13 years, 12 hours
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Re: Fastest Method to Produce A Shroom [Re: HybridprX]
#8031315 - 02/16/08 02:10 PM (15 years, 11 months ago) |
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I don't have much myco experience but I have a good deal of lab experience and moving agar into a non sterile environment, like an FC, for extended amounts of time will increase your contamination greatly (although I use a rich media that has all nutrients not species selective) and agar looses its water very easily. I am sure a more experienced myco will chime in, just my 2Cents.
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Peterthinks
(Caulking) gun for hire


Registered: 11/10/04
Posts: 2,379
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Re: Fastest Method to Produce A Shroom *DELETED* [Re: Hawaiian]
#8031847 - 02/16/08 05:18 PM (15 years, 11 months ago) |
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Post deleted by PeterthinksReason for deletion: waste of time
-------------------- Give a man a fire and he will be warm for the rest of the night. Set a man on fire and he will be warm for the rest of his life. NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS? Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.
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Smushroom
Avid Learner
Registered: 02/02/05
Posts: 2,806
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Re: Fastest Method to Produce A Shroom [Re: Peterthinks]
#8032124 - 02/16/08 06:24 PM (15 years, 11 months ago) |
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If I fruit off agar I'll go better than the PMP. I have an old FC I used for small casings that I plug an ultrasonic into. I may even get a heating pad to put underneath of it to speed up colonization. I would imagine the FC could fit 3x8 petris in it, so 24 diff strains at once. Let them fully colonize with closed (vented) lids, then turn off the heating pad, alcohol the entire inside of the FC, add 2-4 polyfilled holes for FAE, and wait to see pins. Then open the plates and kick the ultrasonic on a couple times a day to keep the humidity up.
If I can fruit directly off agar I may be able to get fruiting time down to ~10 days. That would be great since I could do 3 generations a month.
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: Fastest Method to Produce A Shroom [Re: Smushroom]
#8032244 - 02/16/08 06:57 PM (15 years, 11 months ago) |
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Step To Step 1. Isolates on Agar Media 2. WBS as substrate 3. Colonize one jar and G2G others. 4. Spawn to manure, case, harvest.
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: Fastest Method to Produce A Shroom [Re: Smushroom]
#8032273 - 02/16/08 07:03 PM (15 years, 11 months ago) |
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I think PF tek inoculated with a good amount of LC is probably the best way to go, especially if you have a bunch of isolates you want to grow out. I'm not sure what your ultimate goal is, but the fruits wouldn't be very big off of a shot glass-sized cake, but you could definitely fit a ton of cakes that size into a fruiting chamber.
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Quick WBS Prep
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Peterthinks
(Caulking) gun for hire


Registered: 11/10/04
Posts: 2,379
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Re: Fastest Method to Produce A Shroom *DELETED* [Re: FooMan]
#8032381 - 02/16/08 07:34 PM (15 years, 11 months ago) |
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Post deleted by PeterthinksReason for deletion: waste of time
-------------------- Give a man a fire and he will be warm for the rest of the night. Set a man on fire and he will be warm for the rest of his life. NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS? Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.
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Smushroom
Avid Learner
Registered: 02/02/05
Posts: 2,806
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Re: Fastest Method to Produce A Shroom [Re: Peterthinks]
#8034001 - 02/17/08 08:49 AM (15 years, 11 months ago) |
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dumbfounded: you are looking at atleast 3 weeks for a first flush if not more that way. I want results in under 2.
I have a secret long term goal that I want to work on but mainly I just want to figure this out so I can just test normal isolated strains. Basically if you isolate 100 strains from a single print you could use this to get rid of any non-fruiting strains, you could see which pin the quickest, which produce a certain physical trait (lets say you want to find a new albino strain), or add various nutrients to the media to see which like it best.
I'll keep checking this thread and post results when I actually try it. It will be a few weeks before I get anything isolate though.
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Peterthinks
(Caulking) gun for hire


Registered: 11/10/04
Posts: 2,379
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Re: Fastest Method to Produce A Shroom *DELETED* [Re: Smushroom]
#8034624 - 02/17/08 12:56 PM (15 years, 11 months ago) |
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Post deleted by PeterthinksReason for deletion: waste of time
-------------------- Give a man a fire and he will be warm for the rest of the night. Set a man on fire and he will be warm for the rest of his life. NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS? Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.
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Psuper
Psilocybin


Registered: 02/12/08
Posts: 2,874
Loc: PNW
Last seen: 3 years, 4 months
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Re: Fastest Method to Produce A Shroom *DELETED* [Re: Peterthinks]
#8034979 - 02/17/08 02:38 PM (15 years, 11 months ago) |
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~interesting ideas...thanx guys.~
-------------------- Clinical Management of High Dose Psilocybin Sessions: https://www.youtube.com/watch?v=FbHOTIqjZLk
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