Home | Community | Message Board

MushroomMan Mycology
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Substrate Mix   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleSmushroom
Avid Learner
Registered: 02/02/05
Posts: 2,806
Fastest Method to Produce A Shroom
    #8030209 - 02/16/08 05:40 AM (15 years, 11 months ago)

Now before you flame me let me explain what I am asking. I am starting agar work soon and I have a pretty hefty project in mind but I would really like to fruit each and every substrain I isolate in the process. I don't want to fruit them all bulk because I don't really need that many and I don't have the time to do a large PF grow with normal 1/2 pints.

I am wondering if anyone has experimented with various ways to produce fruits ASAP. I don't care about quantity, a single fruit would be enough to test various traits I would like to isolate.

I have been thinking about 2 options:

1) Fruit directly off of agar. Just have a single plate for each isolate strain. Put them all in a FC and see if they pin. Not sure how well that would work though.

2) The other option is to do a mini-PF Tek grow. I was thinking about using shot glasses. I would imagine with a LC of a single isolate strain it would colonize fully in 3 days, 3 more to pin, 4-5 to get to maturity, maybe less. That means under 2 weeks start to finish. I figure that is the fastest I could ask for.

Anyone else ever tried anything of this nature?


Extras: Filter Print Post Top
OfflineHybridprX
Biodegrader of coir
Male User Gallery


Registered: 01/29/08 Happy 16th Shroomiversary!
Posts: 2,588
Loc: Canada Flag
Last seen: 6 years, 4 months
Re: Fastest Method to Produce A Shroom [Re: Smushroom]
    #8030253 - 02/16/08 06:30 AM (15 years, 11 months ago)

Once the agar is colonized just give it 12 on 12 off of light a day, Ive accidentally had them pin on me while still in the jars from introducing a fully run plate to light to much.

You could just leave a open plate fruiting at room temp, that will work to but you need to be carful that the mycelium does not dry out, so a PmP "Poor mans pod" is advisable, just cutt a two liter pop bottle in half and slide the two sections together, place a damp paper towel in the bottom to help use that moisture to humidify the container rather then have it drawn away from the agar plate itself.


--------------------


Extras: Filter Print Post Top
OfflineHawaiian
mycophile
 User Gallery


Registered: 01/17/08
Posts: 57
Last seen: 13 years, 12 hours
Re: Fastest Method to Produce A Shroom [Re: HybridprX]
    #8031315 - 02/16/08 02:10 PM (15 years, 11 months ago)

I don't have much myco experience but I have a good deal of lab experience and moving agar into a non sterile environment, like an FC, for extended amounts of time will increase your contamination greatly (although I use a rich media that has all nutrients not species selective) and agar looses its water very easily. I am sure a more experienced myco will chime in, just my 2Cents.


Extras: Filter Print Post Top
InvisiblePeterthinks
(Caulking) gun for hire


Registered: 11/10/04
Posts: 2,379
Re: Fastest Method to Produce A Shroom *DELETED* [Re: Hawaiian]
    #8031847 - 02/16/08 05:18 PM (15 years, 11 months ago)

Post deleted by Peterthinks

Reason for deletion: waste of time



--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.


Extras: Filter Print Post Top
InvisibleSmushroom
Avid Learner
Registered: 02/02/05
Posts: 2,806
Re: Fastest Method to Produce A Shroom [Re: Peterthinks]
    #8032124 - 02/16/08 06:24 PM (15 years, 11 months ago)

If I fruit off agar I'll go better than the PMP. I have an old FC I used for small casings that I plug an ultrasonic into. I may even get a heating pad to put underneath of it to speed up colonization. I would imagine the FC could fit 3x8 petris in it, so 24 diff strains at once. Let them fully colonize with closed (vented) lids, then turn off the heating pad, alcohol the entire inside of the FC, add 2-4 polyfilled holes for FAE, and wait to see pins. Then open the plates and kick the ultrasonic on a couple times a day to keep the humidity up.

If I can fruit directly off agar I may be able to get fruiting time down to ~10 days. That would be great since I could do 3 generations a month.


Extras: Filter Print Post Top
Invisibledumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
Re: Fastest Method to Produce A Shroom [Re: Smushroom]
    #8032244 - 02/16/08 06:57 PM (15 years, 11 months ago)

Step To Step
1. Isolates on Agar Media
2. WBS as substrate
3. Colonize one jar and G2G others.
4. Spawn to manure, case, harvest.


Extras: Filter Print Post Top
InvisibleFooMan
 User Gallery

Folding@home Statistics
Registered: 02/02/05
Posts: 8,957
Loc: Earth Flag
Trusted Cultivator
Re: Fastest Method to Produce A Shroom [Re: Smushroom]
    #8032273 - 02/16/08 07:03 PM (15 years, 11 months ago)

I think PF tek inoculated with a good amount of LC is probably the best way to go, especially if you have a bunch of isolates you want to grow out. I'm not sure what your ultimate goal is, but the fruits wouldn't be very big off of a shot glass-sized cake, but you could definitely fit a ton of cakes that size into a fruiting chamber.


--------------------

Quick WBS Prep


Extras: Filter Print Post Top
InvisiblePeterthinks
(Caulking) gun for hire


Registered: 11/10/04
Posts: 2,379
Re: Fastest Method to Produce A Shroom *DELETED* [Re: FooMan]
    #8032381 - 02/16/08 07:34 PM (15 years, 11 months ago)

Post deleted by Peterthinks

Reason for deletion: waste of time



--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.


Extras: Filter Print Post Top
InvisibleSmushroom
Avid Learner
Registered: 02/02/05
Posts: 2,806
Re: Fastest Method to Produce A Shroom [Re: Peterthinks]
    #8034001 - 02/17/08 08:49 AM (15 years, 11 months ago)

dumbfounded: you are looking at atleast 3 weeks for a first flush if not more that way. I want results in under 2.

I have a secret long term goal that I want to work on but mainly I just want to figure this out so I can just test normal isolated strains. Basically if you isolate 100 strains from a single print you could use this to get rid of any non-fruiting strains, you could see which pin the quickest, which produce a certain physical trait (lets say you want to find a new albino strain), or add various nutrients to the media to see which like it best.

I'll keep checking this thread and post results when I actually try it. It will be a few weeks before I get anything isolate though.


Extras: Filter Print Post Top
InvisiblePeterthinks
(Caulking) gun for hire


Registered: 11/10/04
Posts: 2,379
Re: Fastest Method to Produce A Shroom *DELETED* [Re: Smushroom]
    #8034624 - 02/17/08 12:56 PM (15 years, 11 months ago)

Post deleted by Peterthinks

Reason for deletion: waste of time



--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.


Extras: Filter Print Post Top
OfflinePsuper
Psilocybin


Registered: 02/12/08
Posts: 2,874
Loc: PNW
Last seen: 3 years, 4 months
Re: Fastest Method to Produce A Shroom *DELETED* [Re: Peterthinks]
    #8034979 - 02/17/08 02:38 PM (15 years, 11 months ago)

~interesting ideas...thanx guys.~


--------------------
Clinical Management of High Dose Psilocybin Sessions:
https://www.youtube.com/watch?v=FbHOTIqjZLk


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Substrate Mix   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate


Similar ThreadsPosterViewsRepliesLast post
* Anyone know anything about P.C. P.E.S.A. shrooms?
( 1 2 all )
straightbangin 2,592 35 12/03/01 05:56 PM
by Zen Peddler
* Myth about shroom harvest.......
( 1 2 3 all )
badjessejames 27,052 58 07/20/02 07:08 PM
by Zen Peddler
* Should shrooms touch dessicant shroom_assassin 4,115 10 07/16/02 04:51 PM
by straightbangin
* Cutting / breaking shrooms to dry? shroom_assassin 3,808 5 07/20/02 07:05 AM
by ralphster44
* Tales of eating contamed shrooms????????????? abraham 7,185 10 11/28/02 12:50 AM
by Shaw
* Shrooms Growing On Side of MycoBag FredMeyer420 3,379 10 07/20/02 05:57 PM
by Geordi_La_Forge
* drying shrooms Bob_J 6,641 19 11/12/02 04:33 PM
by MOE.shrooms
* Could leaving shrooms w/dessicant 3+ days hurt? shroom_assassin 2,442 5 07/23/02 09:59 PM
by cdlove69

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,428 topic views. 19 members, 171 guests and 56 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.01 seconds on 14 queries.