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poof
Stranger
Registered: 08/07/07
Posts: 209
Last seen: 15 years, 4 months
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One triched grain in 5lb's of incubating hpoo: fucked?
#8028990 - 02/15/08 07:57 PM (15 years, 11 months ago) |
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I pasteurized 160F for two hours, the spawn was immaculate, used the best sterilization practices i could. Ive had to mist daily with a 10% h202 solution to keep it moist while it incubated at 75-80F (been at least a week). Its about 75% colonized when i discovered one "keyboard letter O" sized chunk of triched grain sitting on the top of the sub and carefully removed it. The rest of it looked fine and i checked meticulously, though i obviously have no idea whats happening underneath.
So does this mean its over? I thought the colonized sub was supposed to defend itself.
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HybridprX
Biodegrader of coir



Registered: 01/29/08 
Posts: 2,588
Loc: Canada
Last seen: 6 years, 4 months
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Re: One triched grain in 5lb's of incubating hpoo: fucked? [Re: poof]
#8029010 - 02/15/08 08:02 PM (15 years, 11 months ago) |
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You should still be good for a few flushes, It could have been just that one cluster of kernels. I wouldn't worry about it.
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: One triched grain in 5lb's of incubating hpoo: fucked? [Re: HybridprX]
#8029015 - 02/15/08 08:03 PM (15 years, 11 months ago) |
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we shall see if hybridproxx is correct
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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poof
Stranger
Registered: 08/07/07
Posts: 209
Last seen: 15 years, 4 months
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Re: One triched grain in 5lb's of incubating hpoo: fucked? [Re: tahoe]
#8029102 - 02/15/08 08:29 PM (15 years, 11 months ago) |
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Oh noes, a war has begun | | | V
Edited by poof (02/15/08 08:45 PM)
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auweia
mountain biking


Registered: 12/03/05
Posts: 2,725
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Re: One triched grain in 5lb's of incubating hpoo: fucked? [Re: tahoe]
#8029157 - 02/15/08 08:43 PM (15 years, 11 months ago) |
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Quote:
tahoe said: we shall see if hybridproxx is correct
PLEASE REMOVE MY PHOTOS YOU COPIED FROM MY PRIVATE FLICKR ACCOUNT AND UPLOADED INTO THE PUBLIC DOMAIN WITHOUT MY PERMISSION, TAHOE
http://allyoucanupload.webshots.com/v/2001265624297395721
http://aycu20.webshots.com/image/43659/2004325302560873485_fs.jpg
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xaxphaanes
Mycologist



Registered: 08/08/05
Posts: 2,988
Last seen: 1 year, 5 months
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Re: One triched grain in 5lb's of incubating hpoo: fucked? [Re: auweia]
#8029197 - 02/15/08 08:54 PM (15 years, 11 months ago) |
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once you see trich your project is fucked sorry to say it man but you are fighting a losing battle.
-------------------- "Anything i say is fictional" what you should look for in manure
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HybridprX
Biodegrader of coir



Registered: 01/29/08 
Posts: 2,588
Loc: Canada
Last seen: 6 years, 4 months
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Re: One triched grain in 5lb's of incubating hpoo: fucked? [Re: xaxphaanes]
#8029237 - 02/15/08 09:07 PM (15 years, 11 months ago) |
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Not true, some surface kernels will get hit by trich because the mycelium may have died off from lack of moisture during the incubation period, it happens more often then people think but usally the rest colonize over the few that dont make it.
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xaxphaanes
Mycologist



Registered: 08/08/05
Posts: 2,988
Last seen: 1 year, 5 months
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Re: One triched grain in 5lb's of incubating hpoo: fucked? [Re: HybridprX]
#8029253 - 02/15/08 09:13 PM (15 years, 11 months ago) |
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What is more likely to happen is trich spread all of it spores around his project and will soon germinate and grow.
Another scenario is that the trich is deeply embedded into his substrate since the green is only spores and the mycelium is pure white.
both scenarios means death for the project
-------------------- "Anything i say is fictional" what you should look for in manure
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poof
Stranger
Registered: 08/07/07
Posts: 209
Last seen: 15 years, 4 months
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Re: One triched grain in 5lb's of incubating hpoo: fucked? [Re: xaxphaanes]
#8030480 - 02/16/08 08:51 AM (15 years, 11 months ago) |
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Im going to have to agree with hybrid, it was only a uncolonized grain sitting on the very top that got hit. Xax, i think what you dont realize is that there are trich spores everywhere all of the time. Hell, there are probably thousands in your grow right now. Its really not so much a matter of protecting your grow from bad spores as it is preventing those spores from taking hold, which as we all know is really not a matter of if, but when.
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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Re: One triched grain in 5lb's of incubating hpoo: fucked? [Re: poof]
#8030489 - 02/16/08 08:55 AM (15 years, 11 months ago) |
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dont worry about it until it occurs.. you are just stressing yourself out.. just let them do their thing.. you should get fruits if its just a lil contam and you contained it before it got bad... or it will fuck it... either way, you will have to start over again... lol
just let them grow.. you seemed to have been clean enough, and finding one green chunk (i.e. the truffle shuffle) doesnt mean its the end... haha
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r311ik
n00b


Registered: 01/24/06
Posts: 192
Loc: Mi
Last seen: 15 years, 1 month
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Re: One triched grain in 5lb's of incubating hpoo: fucked? [Re: DJYoshaBYD]
#8030504 - 02/16/08 09:03 AM (15 years, 11 months ago) |
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about a week ago i cut a green spot off a minicake, everything is still good!
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Prisoner#1
Even Dumber ThanAdvertized!


Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
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Re: One triched grain in 5lb's of incubating hpoo: fucked? [Re: poof]
#8030506 - 02/16/08 09:03 AM (15 years, 11 months ago) |
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Quote:
poof said: Im going to have to agree with hybrid, it was only a uncolonized grain sitting on the very top that got hit. Xax, i think what you dont realize is that there are trich spores everywhere all of the time.
xaxphaanes is right, the trich mycelium can run much deeper than you believe, when you see green that simply means that's where the sporulation has begun, keep an eye on your project and you'll probably notice that it will also start in other spots as well and while you're correct in the fact that trich spores are everywhere if you have them in containment like a colonizing jar or a terrarium you have a much heaver concentration of those spore than you would in your whole house or the great outdoors
it will put your current and your future grows at greater risk
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HybridprX
Biodegrader of coir



Registered: 01/29/08 
Posts: 2,588
Loc: Canada
Last seen: 6 years, 4 months
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Re: One triched grain in 5lb's of incubating hpoo: fucked? [Re: Prisoner#1]
#8030547 - 02/16/08 09:20 AM (15 years, 11 months ago) |
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Trich isn't that bad guys, as long as the mycelium runs your bulk you'll be OK.
I spawned Lizard King's - Lizard King strain by sifting through three rye jars by hand in a glovebox, separating the colonized sections from the sections with trich, spawned and still had a successful run and fruit, the prints were not contaminated with Trich either although it can enter the mycelium network, if you case and your casing runs fine then you are good to go. If it turns green, its fubar and disposal is in place.
The only thing I'm scared of is.... insects?
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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Re: One triched grain in 5lb's of incubating hpoo: fucked? [Re: HybridprX]
#8030643 - 02/16/08 09:51 AM (15 years, 11 months ago) |
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I would recommend NEVER messing with trich in the house... its green because it sporulated... if you disturb it, it will let the spores free in your house... thats not a good thing.. you already have enough shit floating through your house.. why add more?
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poof
Stranger
Registered: 08/07/07
Posts: 209
Last seen: 15 years, 4 months
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Re: One triched grain in 5lb's of incubating hpoo: fucked? [Re: DJYoshaBYD]
#8031750 - 02/16/08 04:53 PM (15 years, 11 months ago) |
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Fuck it all.
Edited by poof (02/16/08 05:28 PM)
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Bikerfool
Your Local Edgelord



Registered: 11/21/05
Posts: 1,577
Last seen: 5 months, 13 days
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Re: One triched grain in 5lb's of incubating hpoo: fucked? [Re: poof]
#8031917 - 02/16/08 05:30 PM (15 years, 11 months ago) |
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Quote:
poof said: Fuck me, ive never had this problem before, even when i used to do things real dirty. It seems like the harder i try to keep things clean and sterile the worse off everything gets.
Isn't it funny how that works? When I first started growing I didn't have any problems and didn't know what I was doing. Now that I know exactly what I'm doing everything gets contaminated. I blame it on my new apartment.
-------------------- Just an angsty teen contributing to the pubs decline with contentless posts.
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: One triched grain in 5lb's of incubating hpoo: fucked? [Re: HybridprX]
#8033253 - 02/16/08 11:59 PM (15 years, 11 months ago) |
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Quote:
HybridprX said: Trich isn't that bad guys, as long as the mycelium runs your bulk you'll be OK.
I spawned Lizard King's - Lizard King strain by sifting through three rye jars by hand in a glovebox, separating the colonized sections from the sections with trich, spawned and still had a successful run and fruit, the prints were not contaminated with Trich either although it can enter the mycelium network, if you case and your casing runs fine then you are good to go. If it turns green, its fubar and disposal is in place.
The only thing I'm scared of is.... insects?
And you are the same guy that was telling me that you have been growing for years and now you are telling people to keep thier trich infested substrate. Wow!! You do suck
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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auweia
mountain biking


Registered: 12/03/05
Posts: 2,725
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Re: One triched grain in 5lb's of incubating hpoo: fucked? [Re: tahoe]
#8033268 - 02/17/08 12:05 AM (15 years, 11 months ago) |
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please remove my photos you copied from my Flickr account and uploaded onto the public domain and are using as you're avatar and signature, tahoe
get you're own photos
http://aycu20.webshots.com/image/43659/2004325302560873485_fs.jpg
please remove this photo from the shroomery servers
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