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OfflineCiscoTM
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Registered: 02/15/08
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Last seen: 15 years, 11 months
Help! Conolization gone bad (Lots of Pics Please Help)
    #8027413 - 02/15/08 01:39 PM (15 years, 11 months ago)

First off, long time reader, first time poster. I have tried to go about making the best conolization chamber possible. i have a dark double container with heat controlled growing room. temperatures are around 75.F My growing system showed below is shown below.






The problem is that after 14 days i am seeing little to nothing in conolization. what am i doing wrong? Any ideas? I read to remove the foil on the lids after about 10 days. So i did so on half my jars. as pictured below.






now after 14 days i got about 4 jars that have a little conolization to them, look like they just started. and the rest are nothing, as meaning nothing is going on with them. and 6 jars that are bad allready. pictures show below.









What am i doing wrong. i see very little conolization, the best was with contanimated jars. I was very sterile with my jars. I followed the BRF Tek very accurately.

Please give any advice, info, comments, anything that will help me fix this issue! Thanks ahead of time!


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Offlineitsallnines
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Re: Help! Conolization gone bad (Lots of Pics Please Help) [Re: CiscoTM]
    #8027432 - 02/15/08 01:44 PM (15 years, 11 months ago)

i've had this same thing happen two times in a row. very annoying. i changed vendors.


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by their fruits ye shall know them.


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OfflineCiscoTM
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Registered: 02/15/08
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Re: Help! Conolization gone bad (Lots of Pics Please Help) [Re: itsallnines]
    #8027444 - 02/15/08 01:45 PM (15 years, 11 months ago)

you changed vendors and everything went ok, my vender that i used is not listed on the approved vender's, so i wont say, but i have purchased 3 syringes from spores101 and plan on inulacating soon


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InvisiblePeterthinks
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Registered: 11/10/04
Posts: 2,379
Re: Help! Conolization gone bad (Lots of Pics Please Help) [Re: CiscoTM]
    #8027475 - 02/15/08 01:53 PM (15 years, 11 months ago)

Are those holes still open or taped over? (the injection sites)
also did you use sterile micro pore tape or just plain masking tape?
get the infected jars out of there.boil them for an hour before trying to open them if you want to save the glass.
also was it steam sterilized or PC'd?
the slow ones I wouldn't worry about, they may catch up.
If you have agar I'd test a drop or two from my syringe before I tried again....you could have saved a bit of pain.
if your spore syringe was made from a dirty print there is nothing you could have done to avoid it other than test on agar first.
Better luck next time.
:jester:


--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.


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OfflineCiscoTM
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Re: Help! Conolization gone bad (Lots of Pics Please Help) [Re: Peterthinks]
    #8027497 - 02/15/08 02:00 PM (15 years, 11 months ago)

to answer some of your questions. the holes are tapd with 3" micropore tape. Infected jars are now gone. they were PC'ed for 30 min.

I do not know what an ager is. would like more info.

also a bit more info.

it is very wet in my container. abou t 100% humidy after 10 days i put a lid on the in'er-container to keep the water out....


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OfflineFahkface
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Re: Help! Conolization gone bad (Lots of Pics Please Help) [Re: Peterthinks]
    #8027522 - 02/15/08 02:06 PM (15 years, 11 months ago)

Well, either you have a little too many mold spores flying around your house, or had bad prints / syringes (the second guess would be the one to be preferred!)
In my whole "career" of mushroom cultivation I had only ONE contaminated print and this one came (I know it's mean, but I have to say it) from Spores101 :crazy:

Agar is probably the way to go, to check weather it's your syringes or another source of contams.

I don't know how you're equipped, but you need at least a glove box to work with agar, but you probably know that.

Oh, and BTW: you don't have to wear rubber gloves, when you deal with your jars, or your incubator!
Normally the foil / lid and the dry verm is enough to keep contams away.


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InvisiblePeterthinks
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Registered: 11/10/04
Posts: 2,379
Re: Help! Conolization gone bad (Lots of Pics Please Help) [Re: CiscoTM]
    #8027569 - 02/15/08 02:21 PM (15 years, 11 months ago)

wet is bad.
a damp filter can let stuff thru, especially if you shook the jars and some food got on the filter.
A tub in tub incubator is usually two containers the same size with water a heater and spacers (like small jars) between them. not a smaller tub totally enclosed in a larger one.
The design is at fault probably...sorry.
Agar is the stuff you use to grow clones or spores of mushrooms.
you can get it at Asian markets





Search "Tub in tub" for the right way to do it.
you got most of the stuff already, looks like you just need another big plastic tote and some zip ties.
:jester:


--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.


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OfflineHybridprX
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Re: Help! Conolization gone bad (Lots of Pics Please Help) [Re: Peterthinks]
    #8028061 - 02/15/08 04:31 PM (15 years, 11 months ago)

If you're storing laundry like shirts, sweaters, jackets in with your incubator in a closet even when washed that's when most shit will stick, opening and closing the closet door to check on things sounds like one of your biggest enemys since its causing air currents to pass all those mold spores around and we all know they like their rich, moiste enviorments.

Incubator on a higher shelf is my suggestion or remove all the coats that go into a breeze on a daily basis.


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Offlineitalian1x
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Registered: 06/26/06
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Re: Help! Conolization gone bad (Lots of Pics Please Help) [Re: HybridprX]
    #8028184 - 02/15/08 05:04 PM (15 years, 11 months ago)

I also noticed you said you were incubating at 75F...For incubating in the future, I would probably bump that up 10 degrees. Not only will it colonize much faster, but it will also show contamination (if present) alot faster.


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Offlineismokeweed
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Re: Help! Conolization gone bad (Lots of Pics Please Help) [Re: italian1x]
    #8028303 - 02/15/08 05:28 PM (15 years, 11 months ago)

in response to italians: RogerRabbit did a great test and the perfect temps for incubation are between 75 and 82. it might be 78 to 82. any lower and it will be the same speed as higher, but with higher temps you're more likely to get contams. It was with linear growth in a petri dish. Now so you know most of what he does goes way over my head, but his word to me is like the mushroom bible.

But to you, Cisco, Bumpin it up a few degrees would be good, but I wouldn't say quite ten.


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InvisibleSmushroom
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Registered: 02/02/05
Posts: 2,806
Re: Help! Conolization gone bad (Lots of Pics Please Help) [Re: ismokeweed]
    #8028359 - 02/15/08 05:41 PM (15 years, 11 months ago)

Is the temp in the room or the incubator 75? If it is in the room and you still have the heater going in the TiT then it is too hot.

To me it looks like your temps are too high and are stalling growth. Myc will stall at higher temps more than it will at lower temps. I would suggest not using the heater at all and let it get to room temp. May also be a good idea to leave the lids off to help with gas exchange, lack of oxygen can stall growth as well.


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InvisiblePeterthinks
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Registered: 11/10/04
Posts: 2,379
Re: Help! Conolization gone bad (Lots of Pics Please Help) [Re: ismokeweed]
    #8028418 - 02/15/08 05:53 PM (15 years, 11 months ago)

But check out the pics...there's so much condensation those filters gotta be soggy.Any that aren't crapped yet should be taken out and the filters allowed to dry.
a day at room temp won't kill them.
Hell it takes longer but colonization can all be done at room temperature....just be patient.
Clothes and contams don't mix by the way, I am actually fighting off a lovely skin rash cause I forgot a jar in my dresser.
I am a walking petri dish...go ahead and laugh but just don't do it to yourself.
:jester:


--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.


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OfflineFahkface
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Re: Help! Conolization gone bad (Lots of Pics Please Help) [Re: Peterthinks]
    #8029846 - 02/16/08 12:51 AM (15 years, 11 months ago)

Quote:

I am actually fighting off a lovely skin rash cause I forgot a jar in my dresser.




Haha, classic! :smile:

Anyway, do as the others told you and try another location for your incubation.
Personally I don't think that's the reason why you fail with your jars, because my incubator and FC are stalled in dirtiest room in my house, which is the laundry room. The big benefit of this room, where, although it's pretty damn warm, the humidity is stuck on 18 percent, so bacteria etc. shouldn't feel too well in there.
Best place for drying mushrooms, you could imagine. Cracker dry after a day :tongue2:

Anyway, me don't having any contam problems in there is probably just luck, but since you seem to have an all around contam problem you should consider moving to another room with your incubator.

In the end I think your problem isn't the incubation but in inoculation process. Try other areas for it.
Once I did it in the bathroom, because the kitchen, where I normally do it, was occupied, and all the shitty bacteria etc. have ruined the whole adventure.
Just try all the rooms you have (though i think the best one would be one without a carpet) and give it a try.
Maybe you should, knock up all the jars of one run in all the different rooms to see where it works best. Do that about three times and you should be able to tell, where the chances to succeed are best.


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Offlineatomicblue
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Registered: 11/09/07
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Last seen: 14 years, 2 months
Re: Help! Conolization gone bad (Lots of Pics Please Help) [Re: Fahkface]
    #8029909 - 02/16/08 01:19 AM (15 years, 11 months ago)

1/2 pint jars will colonize faster with out so much moisture that is in the larger pint sized jars. Big cakes!


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