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shymanta
Mad Scientist


Registered: 01/27/05
Posts: 907
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How important is FAE in LC?
#8020498 - 02/13/08 11:48 PM (15 years, 11 months ago) |
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How important is FAE in LC?
When incubating mycelium, it should be in total darkness and it according to The Mushroom Cultivator, Paul Stamets suggests no air exchange is needed.
So, my ultimate question, do you need a filter on an Liquid Culture jar? Is a lid with a simple self-sealing injection point sufficient?
I guess I know the answer, but I'm just looking for consensus.
Thanks all.
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large_dose
Melonhead



Registered: 05/05/06
Posts: 1,346
Loc: Right in the Middle
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Re: How important is FAE in LC? [Re: shymanta]
#8020542 - 02/14/08 12:04 AM (15 years, 11 months ago) |
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No. Dont worry about it.
i just put a hole in the lid of my jar and inoculate. cover with tape or something afterwards.
no filter, non of that shit.
refer to this thread: http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5238137/page/0/fpart/1/vc/1
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Hotsauce72
ShapeShifter



Registered: 07/29/06
Posts: 58
Last seen: 15 years, 10 months
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Re: How important is FAE in LC? [Re: large_dose]
#8020568 - 02/14/08 12:12 AM (15 years, 11 months ago) |
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I could be mistaken, but i think that lc's need air exchange which is different from FAE. I've had success with just a baby bottle tek style lc which included just a self healing silicone injection port.
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shymanta
Mad Scientist


Registered: 01/27/05
Posts: 907
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Re: How important is FAE in LC? [Re: large_dose]
#8020569 - 02/14/08 12:12 AM (15 years, 11 months ago) |
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Nice, thanks.
I remember reading a tek for making lids that had a very elaborate mechanism for injection (silicone) and air flow (polyfill). Since I couldn't find it, I figured the majority discarded it.
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large_dose
Melonhead



Registered: 05/05/06
Posts: 1,346
Loc: Right in the Middle
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Re: How important is FAE in LC? [Re: shymanta]
#8020579 - 02/14/08 12:13 AM (15 years, 11 months ago) |
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Gas Exchange & FAE are two different things.
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shymanta
Mad Scientist


Registered: 01/27/05
Posts: 907
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Re: How important is FAE in LC? [Re: large_dose]
#8020603 - 02/14/08 12:18 AM (15 years, 11 months ago) |
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That's what I get for my lazy use of abbreviations. In any case, is oxygen (more than what exists in the water and air before sealing) needed?
I like this tek. Its simple and does not include gas exchange. http://www.shroomery.org/9666/Cybers-Self-Healing-Injection-point-lids
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P.Menace
Contradicting the Status Quo




Registered: 11/17/07
Posts: 4,797
Loc: Let Me See Potato Salad!!...
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Re: How important is FAE in LC? [Re: shymanta]
#8021004 - 02/14/08 03:09 AM (15 years, 11 months ago) |
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gas exchange isnt as important in an lc as it is in a PF or WBS jar, the most exchange needed can be achieved by punching a 1/8" hole in the center of the lid (for inoculation) and after inoculation cover the hole with the sticky part of a BRAND NEW band-aid as it will be sterile when its new and fresh out of the package. Personally if i didnt need a hole to inoculate thru (you really dont, but its more contaminate risky) I would use a solid lid to allow for shaking to break up the mycelium
--------------------
http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: How important is FAE in LC? [Re: P.Menace]
#8021392 - 02/14/08 08:32 AM (15 years, 11 months ago) |
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the filter offers 2 things. the most important thing is that it filters the air that is sucked in while you pull out the lc. The empty space has to be replaced with something. If you have negative pressure in there eventually you would not be able to suck the lc out.
prox or hydridprox says that lc's must have fresh air exchange or they will die. He says that you have to inject the lc with o2 via the syringe
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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doc34
Fungitarian



Registered: 02/14/04
Posts: 2,667
Loc: Myceliaville !!!
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Re: How important is FAE in LC? [Re: tahoe]
#8021419 - 02/14/08 08:41 AM (15 years, 11 months ago) |
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Quote:
the filter offers 2 things. the most important thing is that it filters the air that is sucked in while you pull out the lc. The empty space has to be replaced with something. If you have negative pressure in there eventually you would not be able to suck the lc out.
I agree!
You don't need air exchange so the mycelia can breathe or so that gasses can excape, but, you do need it for the reason Tahoe stated. This only applies to an LC though, not your spawn jars(you know that of course).
Doc
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doc34
Fungitarian



Registered: 02/14/04
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Re: How important is FAE in LC? [Re: tahoe]
#8021476 - 02/14/08 08:55 AM (15 years, 11 months ago) |
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Quote:
the filter offers 2 things. the most important thing is that it filters the air that is sucked in while you pull out the lc. The empty space has to be replaced with something. If you have negative pressure in there eventually you would not be able to suck the lc out.
I agree!
You don't need air exchange so the mycelia can breathe or so that gasses can excape, but, you do need it for the reason Tahoe stated. This only applies to an LC though, not your spawn jars(you know that of course).
Doc
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Neobean
Adept Mycologist


Registered: 10/07/01
Posts: 975
Loc: Canada
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Re: How important is FAE in LC? [Re: doc34]
#8021478 - 02/14/08 08:56 AM (15 years, 11 months ago) |
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Quote:
doc34 said:
Quote:
the filter offers 2 things. the most important thing is that it filters the air that is sucked in while you pull out the lc. The empty space has to be replaced with something. If you have negative pressure in there eventually you would not be able to suck the lc out.
I agree!
You don't need air exchange so the mycelia can breathe or so that gasses can excape, but, you do need it for the reason Tahoe stated. This only applies to an LC though, not your spawn jars(you know that of course).
Doc
Yup Theoretically you have enough air in the remainder of the jar (where the LC isn't) for the LC to live. But a gas exchange hole never hurt any LC...
-------------------- If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!
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Her0
Stranger



Registered: 11/06/07
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Re: How important is FAE in LC? [Re: Neobean]
#8021798 - 02/14/08 10:27 AM (15 years, 11 months ago) |
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i just had success with my first try at a honey lc. the lid of the jar has been on tight for weeks now and there is a nice cloud of mycelium floatin round in there
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zyxwvutsr
rstuvwxyz


Registered: 12/30/07
Posts: 141
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Re: How important is FAE in LC? [Re: tahoe]
#8022000 - 02/14/08 11:22 AM (15 years, 11 months ago) |
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Quote:
tahoe said: prox or hydridprox says that lc's must have fresh air exchange or they will die.
prox or hydridprox is wrong. If this were true My 2 year old vacutainer LC's wouldn't be good, and they are.
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: How important is FAE in LC? [Re: zyxwvutsr]
#8022150 - 02/14/08 12:05 PM (15 years, 11 months ago) |
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believe me I know this. Proxx/hybridprox is an idiot. Too bad he has me on ignore so he will never see this. He also suggest pc'ing grain for 2 hours but this is after you soak for 36 hours, pc for 2 hours, soak again for 12 hours and pc it for a second time for 90 mins
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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RoYaL_fLuSh
Stranger


Registered: 01/04/08
Posts: 499
Loc: UK
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Re: How important is FAE in LC? [Re: tahoe]
#8022173 - 02/14/08 12:11 PM (15 years, 11 months ago) |
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hmm u mean the same hybridx that gave me a 0 rating talking chit when i dont even know wot the fuk his issue is ? that 1 ? lol yah hes an idiot and talks out his azz ! sorry .. off topic .. please continue ..
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obscured
Bloodletting


Registered: 02/01/08
Posts: 206
Last seen: 3 years, 8 months
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Re: How important is FAE in LC? [Re: RoYaL_fLuSh]
#8022358 - 02/14/08 01:03 PM (15 years, 11 months ago) |
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On topic, I'm trying something new with my LC
Silicone injector site. Silicone site on other side with a polyfil syringe. PC it all together, with foil covering the top of the syringe.
I'm just gonna leave that syringe in there indefinably. Should allow me to innoc and withdraw solution without too much of an issue.
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