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Offlineikkes
very sad

Registered: 02/07/08
Posts: 98
Last seen: 14 years, 6 months
Not Fully grown myc..... Dunk?
    #8017143 - 02/13/08 11:13 AM (15 years, 11 months ago)

Seems like two cakes (little overheated and bruised) are not growing anymore.

Rinse of the remaining BRF + vermiculite and dunk, or not?

If not any tips and tricks


--------------------
..........3 Tage wach..........
:crazy2: :crazy2: :crazy2: :crazy2: :crazy2:
jetzt wirst du langsam schwach


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OfflineiTrip
Electronic Trip
Male


Registered: 11/21/07
Posts: 68
Last seen: 14 years, 6 months
Re: Not Fully grown myc..... Dunk? [Re: ikkes]
    #8017164 - 02/13/08 11:19 AM (15 years, 11 months ago)

how long ago did u inoc?

and u say bruised.? how bruised is it blue or greyish?

pics would really help u know/.


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Offlineikkes
very sad

Registered: 02/07/08
Posts: 98
Last seen: 14 years, 6 months
Re: Not Fully grown myc..... Dunk? [Re: iTrip]
    #8017451 - 02/13/08 12:43 PM (15 years, 11 months ago)

inoculated

5days after growth start
2 weeks after 80% covered
4 weeks after not much more happend and I figured out that in the afternoon temperature was above the recommenden temperature (probably that stopped growth)
I think we are at 4.5weeks after first growth started

problem is that it are semi transparent flexible plastic jars..... came with the kit
So a pic doesn't say much, it will be A blurred pic

when I noticed growth stopped, at first I was concerned maybe it is contaminated, so i dented the jar somewhat and the spots where I dented it now you see some blue/greyish spots (don't seem to grow worse..)
plus it starts to look a little dryer in the jar as time goes


--------------------
..........3 Tage wach..........
:crazy2: :crazy2: :crazy2: :crazy2: :crazy2:
jetzt wirst du langsam schwach


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Offlineasknoquestions
Stranger

Registered: 01/16/08
Posts: 97
Last seen: 14 years, 4 months
Re: Not Fully grown myc..... Dunk? [Re: ikkes]
    #8017579 - 02/13/08 01:13 PM (15 years, 11 months ago)

It is likely that there is contamination, not in the colonized portion, but in the uncolonized part.

Are the lids sealed? It is also possible that the substrate was mixed too wet.


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Offlineikkes
very sad

Registered: 02/07/08
Posts: 98
Last seen: 14 years, 6 months
Re: Not Fully grown myc..... Dunk? [Re: asknoquestions]
    #8017672 - 02/13/08 01:28 PM (15 years, 11 months ago)

Lids are sealed

to wet.... it is possible, just followed instructions that came with the order, being a first timer.

My cousin ordered at same shop, same as me he has a background in microbiology so sterility was no problem..
But he disregarded every instruction, mixed the amounts and weighed on a crappy scale, not fully dark incubation temperature fluctuating like hell etc etc
He got 60g dry of a few cakes, no contams.. within 5 weeks

I tried to create ideal environment ........ and look what happens


I'll just open the two jars (it's only two.. ) swab them if the blue stuff is really bruising, smell it and
then rinse and dunk..... worst case I loose the two cakes
still have more than enough spores.


--------------------
..........3 Tage wach..........
:crazy2: :crazy2: :crazy2: :crazy2: :crazy2:
jetzt wirst du langsam schwach


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