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Offlinejonogt
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Registered: 12/27/07
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LC - contams or leftover clumps of spores?
    #8016012 - 02/13/08 12:43 AM (16 years, 7 days ago)

pint jars, ~3% honey solution in distilled water, started from spores, polyfil filters, magnetic stirrer agitation.

I'm indecisive on what I see and its because of the spores I used. The syringe was REALLLY CHUNKY with em... jar 1 of 2 i nocced I watched several pretty big clumps pass into the needle, and jar 2 of 2 I had to give the plunger a bit of real effort before it pretty much launched a huge ball of em in. So my jars had big clumps of spores floating around in them from the start. I also had some honey sediment that I didn't filter based on good things people said about it giving the myc something to grab onto.

I'm at day 3 currently, and the jars look to be colonizing pretty well with a light milky suspension that's nearly homogenous through the whole jar. I usually just put it on for a minute or 2 stir on real low speed now to throw everything around.

However, I just realized a few minutes ago that I'm still seeing black pieces in the jars, but they are now looking more like 2D flakes now instead of 3D chunks. They are almost completely solid pieces... not translucent or networky like mycellium at all, and they sit almost exclusively at the bottom unless the jar is stirred up. They don't seem to be affecting the mycellial growth in any way.

You guys think these are still spores just being stubborn, or can someone take a guess at what other fungus (if any) this might be? can the spores gunk up with the honey sediment and proteins and stuff to do this?

currently I think more can be had out of what i just explained than the pictures attached... the biggest ones in there are just over 1/16" wide, and I just can't get a very good photo.

but heres the album for what you can see.
http://smg.photobucket.com/albums/v171/Jonogt/

under 10x handheld mag I see quite a few of them with pieces of white stuck to them and near them and branching away from them etc... aren't those desirables just getting going on their myc production?

if an LC is kinda overfilled with spores (these would probably be considered that if there is such a thing), can a given volume hit a working load limit where there just can't be anymore myc growth sites in suspension, and as a result leave the spores that theres no room for at the bottom?



I'm sure I sound like I'm trying to justify why I don't have contams, which is fairly true, but do tell me what you guys think.

thanks
-Jon


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Invisibletahoe
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Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: LC - contams or leftover clumps of spores? [Re: jonogt]
    #8016061 - 02/13/08 12:55 AM (16 years, 7 days ago)

probably clumps of spores. spores will clump together on the bottom.
The pics suck though. The lc is hazey. has it always been this hazey?
If it gets milky or hazey i would trash it


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Invisibletahoe
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Re: LC - contams or leftover clumps of spores? [Re: tahoe]
    #8016064 - 02/13/08 12:56 AM (16 years, 7 days ago)

see this lc is clear minus the mycelium. tehre is no haze to it
it is just over grown



--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Offlinejonogt
Jon
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Registered: 12/27/07
Posts: 104
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Re: LC - contams or leftover clumps of spores? [Re: tahoe]
    #8016101 - 02/13/08 01:04 AM (16 years, 7 days ago)

the lighting and jar makes it look much more like that than it is. the honey is a little cloudy, but most of what you actually see if you hold it up to light I'm assuming is the early mycellium: tons of finer strands more evenly suspended in the liquid that just havn't formed cloud-like pieces yet. It's still pretty translucent.

can't do any better on the photos with my currently available camera unfortunately. it's over 6 years old, 1.3 mp, and the jar were giving a pretty rough glare.

thanks for the input on the clumps
-Jon


Edited by jonogt (02/13/08 01:15 AM)


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OfflineMayorMcCheese
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Registered: 11/10/07
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Last seen: 8 years, 3 months
Re: LC - contams or leftover clumps of spores? [Re: tahoe]
    #8016374 - 02/13/08 05:29 AM (16 years, 6 days ago)

Quote:

tahoe said:

If it gets milky or hazey i would trash it



Any idea what kind of contam the milky stuff might be?


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Invisibleblood4blood
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Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: LC - contams or leftover clumps of spores? [Re: MayorMcCheese]
    #8016400 - 02/13/08 06:28 AM (16 years, 6 days ago)

could be anything. like tahoe says when you make an lc with a spore syringe there is a huge amount of area for the risk of contamination. sometimes they work, sometimes they dont. i just finially got one to work via spore syringe. it was my third try.


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