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Offlinedl46
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Can the mycelium win?
    #8012698 - 02/12/08 10:26 AM (15 years, 11 months ago)

A couple of my jars have become contaminated with what looks like bacterial colonies. Below are some pictures (the black lines are mark I drew on the outside of the glass to see if the mycelium was still growing).

Jar #1:





Jar #2:



Now, even though the bacterial colonies are clearly visible (especially in the close-up of jar #1 above) the jars don't smell bad and the mycelium seems to be slowly but surely encroaching upon the infected areas - it seems to be winning the battle, particularly in jar #2. Obviously I've separated these two jars from the others, but my question is, should I chuck them or let this play out? Can the mycelium possibly win this battle against the bacteria? I assume that sometimes shrooms must win, or else how would they survive in the wild...


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OfflineGroomies
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Re: Can the mycelium win? [Re: dl46]
    #8012707 - 02/12/08 10:29 AM (15 years, 11 months ago)

well if you don't mind starting over. just chuck it outside somewhere and see if you could get a nice outdoor grow.


--------------------
:hotidea::hotidea::hotidea:


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Offlinedl46
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Re: Can the mycelium win? [Re: Groomies]
    #8012746 - 02/12/08 10:38 AM (15 years, 11 months ago)

Hmmmm, given that it's several degrees below zero outside, I don't rate my chances of success at outdoor growing.

Has anyone ever known shrooms to recover from any sort of contamination? Do they ever win?


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Offlineudok
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Re: Can the mycelium win? [Re: dl46]
    #8013856 - 02/12/08 04:42 PM (15 years, 11 months ago)

Why do you think that it is a bacterial contamination in an BRF cake?
For me it looks like
a) to much water
b) temperature fluctuations (from to high?) Should be 75F to 81F


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Offlinedl46
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Re: Can the mycelium win? [Re: udok]
    #8017298 - 02/13/08 12:03 PM (15 years, 11 months ago)

I am certain that it is bacterial contamination for four reasons:

1) The mycelium has rapidly covered the whole rest of the cakes, leaving a single section on each cake bizarrely uncolonised.

2) I can clearly see bacterial colonies on the exposed substrate. They are milky-white to pale-brown translucent blobs surrounding the vermiculite particles.

3) I have shown the jars to a biologist friend of mine, who agrees that they are definitely bacterial colonies.

4) I have already trashed another jar which had an identical bacterial infection, but was much less colonised with mycelium. When I took the lid off it, the smell was extremely unpleasant.

I am aware that there is a significant amount of condensation in the jars, but there is a visible difference between clear condensation on the colonised portions of the cake and the translucent blobs of bacteria growing on the uncolonised sections. The cakes are being incubated at 80F constant temp.


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Offlineudok
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Re: Can the mycelium win? [Re: dl46]
    #8017799 - 02/13/08 01:53 PM (15 years, 11 months ago)

Quote:

dl46 said:
2) I can clearly see bacterial colonies on the exposed substrate. They are milky-white to pale-brown translucent blobs surrounding the vermiculite particles.




So the whitish spots are not partly grounded rice as i thought.
The only 'pale-brown' liquid i see is a fungal metabolic product.


--------------------
And on the 7. day the creator designed the psychedelic drugs. Holy shit. Thats intelligent design far beyond my scope. :wink:
Namaste


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OfflineThe shroomy 1
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Re: Can the mycelium win? [Re: udok]
    #8019935 - 02/13/08 10:02 PM (15 years, 11 months ago)

I tend to side with udok. Those are very nice close up pics of your jars and the only thing I see is over condensation. The places where I could possibly say that looks like "blobs of bacteria" look like very wet BRF. I don't see any slimy look to it at all! I would think that if there was that much bacteria to prevent that much space to be uncolonized due to it, you would definitely smell it! I'm thinking there's no contamination. Give it a week.


--------------------


AMU Q&A thread.


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Invisibleim_on_a_boat
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Re: Can the mycelium win? [Re: The shroomy 1]
    #8020040 - 02/13/08 10:19 PM (15 years, 11 months ago)

yup that's what too-wet brf grains look like from my experience..

but my jar stalled at about the same spot then that whole part turned green/black so there's no telling..

the OP might be right.. we can only tell so much from pictures.


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InvisibleSLASHpro
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Re: Can the mycelium win? [Re: im_on_a_boat]
    #8022970 - 02/14/08 03:56 PM (15 years, 11 months ago)

yeah it looks like its just too wet. i don't see any kind of mold/bacteria, but its hard to tell even with pictures. maybe you should try loosening the lid to allow some air flow and/or flipping the jar over and see what happens in a week. good luck!


--------------------
"All that you touch, see, taste, feel, love, hate, distrust, save, give, deal, buy, beg, borrow, or steal,
all you create, destroy, do, say, all that you eat, and everyone you meet,
all that you slight, and everyone you fight, all that is now, all that is gone, all that's to come,
and everything under the sun is in tune, but the sun is eclipsed by the moooooooooooooon!"
- Roger Waters :darkside:


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Offlinedl46
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Re: Can the mycelium win? [Re: SLASHpro]
    #8027135 - 02/15/08 12:38 PM (15 years, 11 months ago)

I loosened the top of the jars and removed the foil, and now I can smell the infection. In the jar which was severely infected, the globules of bacteria are getting out of hand and the mycelium has made no progress whatsoever, so I'm going to chuck it. The other jar, however, has made some impressive progress since I took it out of the incubator - I think the lower temperature has given the mycelium the advantage:



If you compare that pic to the one at the top, you can see that the mycelium is definitely taking control of the contaminated area, bit by bit. I'm gonna leave it and see what happens as this unfolds. Looks to be winning though!


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Offlineudok
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Re: Can the mycelium win? [Re: dl46]
    #8028123 - 02/15/08 04:47 PM (15 years, 11 months ago)

Sorry for you:(
The disadvantage of higher than room temperture 'incubation'
is that one can get much more condensationn water.And that is nice for
bacteria, but not for the wanted mycelium (i have read,and proven)


Edited by udok (02/15/08 10:27 PM)


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