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OfflineTheFish
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Agar to Liquid Culture. How much tissue to transfer?
    #8012577 - 02/12/08 09:41 AM (15 years, 11 months ago)

I'm ready to transfer a wedge of colonized agar to a culture jar.

My question is, how much should I transfer? With the viscosity of agar at room temperatures, I figure just a small wedge so it won't clog the needle when I aspirate it.

Thanks my blueberry muffins.


--------------------
"Comes a time when the blind man takes your hand,
Says, 'Don't you see?'
Gotta make it somehow on the dreams you still believe.
Don't give it up.
You got an empty cup only love can fill."

Jerry Garcia


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OfflineGroomies
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Re: Agar to Liquid Culture. How much tissue to transfer? [Re: TheFish]
    #8012596 - 02/12/08 09:45 AM (15 years, 11 months ago)

just a small amount is enough. it will grow inside the LC.


--------------------
:hotidea::hotidea::hotidea:


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Invisibletahoe
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Registered: 11/26/03
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Re: Agar to Liquid Culture. How much tissue to transfer? [Re: TheFish]
    #8012598 - 02/12/08 09:46 AM (15 years, 11 months ago)

I useually transfer a 1cm square to lc. But this is what i do. I cut the square but I do not cut the agar, i just cut the surface. i also cut this peice into 10+ little slivers. Then I scrape the mycelium off the agar. its just a ball of mycelium and I dump it in. once it hit the liquid I swirl it and it break up and now I have a shit load of little pieces in there.

Ther is no need for anymore than that


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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OfflineTheFish
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Registered: 10/07/03
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Re: Agar to Liquid Culture. How much tissue to transfer? [Re: tahoe]
    #8012660 - 02/12/08 10:10 AM (15 years, 11 months ago)

:thumbup:


--------------------
"Comes a time when the blind man takes your hand,
Says, 'Don't you see?'
Gotta make it somehow on the dreams you still believe.
Don't give it up.
You got an empty cup only love can fill."

Jerry Garcia


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