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OfflinePitcherCrab
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Question about LCs
    #8010188 - 02/11/08 07:02 PM (15 years, 11 months ago)

I was wondering if it is necessary to use distilled water when making LCs with the intent of using them for culture storage. I have been making all my LCs with tap water and haven't had any problems so far, but I just got a bunch of vacutainers and am planning to store my cultures in them using the distilled water technique. I am going to fill them up with LC, then once the mycelium has sunk to the bottom of the tube suck the honey water out and replace it with distilled water. Will it matter that the last little bit of LC was made with tap water?


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“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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Offlinewoefuljungle
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Re: Question about LCs [Re: PitcherCrab]
    #8010407 - 02/11/08 07:35 PM (15 years, 11 months ago)

when the next person posts would u mind telling me what a lc is? not sure i know


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OfflinePitcherCrab
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Registered: 12/02/06
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Re: Question about LCs [Re: woefuljungle]
    #8010455 - 02/11/08 07:43 PM (15 years, 11 months ago)

Quote:

woefuljungle said:
when the next person posts would u mind telling me what a lc is? not sure i know




LC= liquid culture


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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Invisibleblood4blood
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Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: Question about LCs [Re: PitcherCrab]
    #8010726 - 02/11/08 08:22 PM (15 years, 11 months ago)

if you boil/sterilize tap water using it would be just fine.

i wouldnt replace water in your vactionaters with just plain distilled water. i would boil it let it cool and then use it


Edited by blood4blood (02/11/08 08:23 PM)


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