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OfflineBrainSalad
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Registered: 02/04/08
Posts: 9
Last seen: 15 years, 2 months
About to start: Small questions/clarifications and do I have everything?
    #8000321 - 02/09/08 02:09 PM (15 years, 11 months ago)

I'm about to start sometime in the next day or two here and I have some questions.

First, let me start off: I HAVE read the FAQs/guides and such, as well as a lot of forum posts (been lurking here for a bit lately). Now, there are some things I am a bit unclear about a few things:

Preliminaries:

Starting off with two 5lb Manure/nutrient spawn bags (presterilized)
B+ Spore Syringe (10CC)
PF Classic Syringe (10cc)
Tub-type tote kept in closet for incubating.

---

1. What temperature should I aim for?! I keep hearing things from "normal room temperature" to 80 degrees (optimal being 82-84, but the mycelium should supposedly add a few degrees to the temperature?)

So what should I aim for? at the moment I have no way to heat up the incubation area easily, but could macguyver something up here if I need to.


----
2. How many CCs should I add per bag? I purchased some syringes from a reputable vendor who has a reputation for very nice, dark syringes. And indeed, it is quite dark and there are clumps of spores everywhere.
I read that too dark of a solution can be detrimental (too many different spores competing for the same substrate or something?).

Anyway, taking these exceedingly dark prints into account and my somewhat larger than normal 5 lb bags, how much solution should I use per bag? I don't mind emptying a syringe if I need to or anything, but I'm trying to go for optimum mushroom growth/best colonization.

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3. I've got my bags, syringes, lysol (to clean out the box before injecting the bags and incubating them), gloves, iso, a mini-torch, and a lot of patience. Do I need anything else at the moment? I'm going to decide if I'm going to case or not (leaning toward doing so because I want some decent yields, hopefully), so I still have to get coco and such if I want to do that, but I have at least 2-3 weeks before colonization anyway, I imagine.
---
4. I stored the bag mixture in the fridge overnight, how long should I wait until inoculating them? Or does the slightly cooler temperature of the manure not matter as long as it eventually stabilizes to room temp?

That's really actually all I had wanted to ask about at the moment. I know these questions have been asked before, but I couldn't come to a straight answer/conclusion.

Sorry if I missed a definitive answer to one of these questions in my searching!


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OfflineYossarian22
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Registered: 09/12/07
Posts: 415
Last seen: 9 years, 1 month
Re: About to start: Small questions/clarifications and do I have everything? [Re: BrainSalad]
    #8000408 - 02/09/08 02:31 PM (15 years, 11 months ago)

Assuming you live in a comfortably warm house/dwelling, room temperature should be fine. If you can live comfortably in a room with a T-shirt on, the mycelium will be just fine. Higher temperatures speed up colonization to a degree, but they also increase your contamination risk.

You might want to get some micropore tape- you can find it in most pharmacies, usually marketed as "Gentle Paper Tape". If your spawnbag has a self-healing injection port, you don't need to worry about it. If it doesn't, swab some rubbing alcohol on the micropore tape(don't soak it, though) and put the micropore tape over the injection site as soon as you pull the needle out. Other than that, it seems like you're all set. I'd recommend casing with peat instead of coir since coir is very nutritious and can cause problems when the mycelium tries to use it like substrate. But everyone has their own opinion on the best casing materials.

I wouldn't worry about the fridge- mycelia and spores can both survive cool temperatures. At worst, it'll slow down growth for a day or so.


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OfflineSeventy
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Registered: 05/24/07
Posts: 912
Loc: Canada (PNW)
Last seen: 6 months, 28 days
Re: About to start: Small questions/clarifications and do I have everything? [Re: BrainSalad]
    #8000421 - 02/09/08 02:36 PM (15 years, 11 months ago)

1. You can incubate anywhere from 74F higher, but I wouldn't go above 80F because you're asking for more contams that way. I let everything I grow colonize at around 74-77F and all works fine.

2. A clear syringe will work just as good as a dark syringe. Spores are smaller than you can see with the naked eye anyways. I don't have any experience with bags, but I'd say anywhere from 1.5-2.5cc's per bag.

4. You can inoculate them while they're cool or you can let them adjust to room temperature, I don't think it really matters, either way they'll end up at the temperature you're incubating at. The spores won't care.


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OfflineBrainSalad
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Registered: 02/04/08
Posts: 9
Last seen: 15 years, 2 months
Re: About to start: Small questions/clarifications and do I have everything? [Re: Seventy]
    #8000504 - 02/09/08 02:52 PM (15 years, 11 months ago)

Okay, thanks for the help guys!

I'm going to inoculate them in a bit, hopefully. Just cleaning up the area where they'll be spending the next few weeks (hopefully).


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