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OfflineFlurpes
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Registered: 01/19/08
Posts: 20
Last seen: 15 years, 9 months
Did I just screw up badly
    #7989293 - 02/06/08 09:32 PM (15 years, 11 months ago)

Today I received my mushroom spore syringe and began using the PF-tek to grow some mushrooms. I memorized the whole process basically and only checked on it for details but I missed the part where it says that you have to use a different lighter then a cigarette lighter. Will using a cigarette lighter kill the spores or infect the cake?


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Invisibleblood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: Did I just screw up badly [Re: Flurpes]
    #7989311 - 02/06/08 09:35 PM (15 years, 11 months ago)

if your got the needle red then there should be no problem


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Offline81Renaissance
Mad Scientist
Male


Registered: 11/01/06
Posts: 457
Last seen: 10 years, 10 months
Re: Did I just screw up badly [Re: Flurpes]
    #7989316 - 02/06/08 09:36 PM (15 years, 11 months ago)

Cigarette lighter just causes the needle of the syringe to get sooty b/c the butane doesn't burn clean. It should be fine, but when I did that, I wiped the syringe with alcohol before innoculating just to be sure...still think you're okay, but only time will tell.


--------------------
"Every passing moment is another chance to turn it all around."


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OfflineEat The Rich
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Registered: 10/01/07
Posts: 23
Last seen: 15 years, 2 months
Re: Did I just screw up badly [Re: Flurpes]
    #7989323 - 02/06/08 09:37 PM (15 years, 11 months ago)

Theres no issues here, what tek told you not to use a cig. lighter?

And, btw, I love, absolutely love the title to your thread.


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Invisibletahoe
Noob Slayer
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Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: Did I just screw up badly [Re: Eat The Rich]
    #7989395 - 02/06/08 09:49 PM (15 years, 11 months ago)

you should be fine. In theory soot would have better odds of being free of contams than a alcohol wipe. I would never touch the needle after flaming it


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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OfflineFlurpes
Stranger

Registered: 01/19/08
Posts: 20
Last seen: 15 years, 9 months
Re: Did I just screw up badly [Re: Eat The Rich]
    #7989413 - 02/06/08 09:51 PM (15 years, 11 months ago)

I got the tek here, it just said its "undesirable" which I didn't know exactly what that meant. Also I put the jars in a cloths bin and up on a bed with the lid open. There is a window next to the bed but the window is lower then where the bin is on the bunk bed (top bunk). My question is should I leave the bin open all the time and should I worry about having the jars closer to the window to get more light?


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Invisibletahoe
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Registered: 11/26/03
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Re: Did I just screw up badly [Re: Flurpes]
    #7989440 - 02/06/08 09:57 PM (15 years, 11 months ago)

light is not important yet, Just put the cakes somewhere and do not fuck with them for 2 weeks. Pace yourself. You will be so stoked to see quarter sized white dots on all your cakes in two weeks and you will not have lowered their survival rate


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Offline81Renaissance
Mad Scientist
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Registered: 11/01/06
Posts: 457
Last seen: 10 years, 10 months
Re: Did I just screw up badly [Re: Flurpes]
    #7989442 - 02/06/08 09:57 PM (15 years, 11 months ago)

You want total darkness for incubation! Light shouldn't be introduced until the fruiting phase, and if possible, get it away from the window...you want it to be at or above room temp. for colonization. Warmer is better even if its just a few degrees, mycelium colonization is exothermic so they will generate a little of their own heat.


--------------------
"Every passing moment is another chance to turn it all around."


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OfflineFlurpes
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Registered: 01/19/08
Posts: 20
Last seen: 15 years, 9 months
Re: Did I just screw up badly [Re: 81Renaissance]
    #7989471 - 02/06/08 10:05 PM (15 years, 11 months ago)

So I should put the lid on? If I do there won't be any circulation of air in the container. Should I use cloth to keep it in darkness without the lid so it can get air or just keep them in there with the lid on and leave it alone?


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Invisibletahoe
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Re: Did I just screw up badly [Re: Flurpes]
    #7989481 - 02/06/08 10:07 PM (15 years, 11 months ago)

are their dirty clothes going on top of them??
They will have enough fresh air with the lid on.
Put them back in the box that they come in and leave them alone.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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OfflineFlurpes
Stranger

Registered: 01/19/08
Posts: 20
Last seen: 15 years, 9 months
Re: Did I just screw up badly [Re: tahoe]
    #7989501 - 02/06/08 10:10 PM (15 years, 11 months ago)

Its just a regular clothes bin that I had, they didn't come in a container. Dirty clothes won't be going on top or anything. The jars are just sitting in there. You think they will have enough air with the lid on? It just snaps on not sure how air tight it is.


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Invisibletahoe
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Re: Did I just screw up badly [Re: Flurpes]
    #7989506 - 02/06/08 10:11 PM (15 years, 11 months ago)

absolutely. The jars do not need much o2.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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OfflineFlurpes
Stranger

Registered: 01/19/08
Posts: 20
Last seen: 15 years, 9 months
Re: Did I just screw up badly [Re: tahoe]
    #7989569 - 02/06/08 10:28 PM (15 years, 11 months ago)

Awesome, thanks for the replies guys. Like always it helped me a lot.


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Offlinebeatyou
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Registered: 02/21/06
Posts: 618
Loc: austin, tx
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Re: Did I just screw up badly [Re: Flurpes]
    #7990265 - 02/07/08 02:06 AM (15 years, 11 months ago)

lighter is fine, just get red hot, let cool for about 5 seconds so you don't destroy spores as they come out of the syringe.

alcohol lamp is ideal, if you can get one.


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Offlinegaladar


Registered: 05/09/07
Posts: 981
Loc: in a monotub!
Last seen: 2 years, 2 months
Re: Did I just screw up badly [Re: tahoe]
    #7990583 - 02/07/08 05:54 AM (15 years, 11 months ago)

Quote:

tahoe said:
you should be fine. In theory soot would have better odds of being free of contams than a alcohol wipe. I would never touch the needle after flaming it




tru, a friend of a friend just sticks the flaming hot needle into the jar(pf tek) where it is sterile and shoots after a second or two.

foolproof. and safer in theory.

he even did it with polyfil, it burns the polyfil a little but worked to our amazement.


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OfflineNeobean
Adept Mycologist
Male

Registered: 10/07/01
Posts: 975
Loc: Canada
Last seen: 3 years, 8 months
Re: Did I just screw up badly [Re: 81Renaissance]
    #7990733 - 02/07/08 08:04 AM (15 years, 11 months ago)

Quote:

81Renaissance said:
You want total darkness for incubation!




LIES!

Light has no impact, negative or positive on the colonization of jars.


--------------------
If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!


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Offlineshevanel
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Registered: 01/27/08
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Re: Did I just screw up badly [Re: galadar]
    #7990755 - 02/07/08 08:18 AM (15 years, 11 months ago)

Quote:

galadar said:
Quote:

tahoe said:
you should be fine. In theory soot would have better odds of being free of contams than a alcohol wipe. I would never touch the needle after flaming it




tru, a friend of a friend just sticks the flaming hot needle into the jar(pf tek) where it is sterile and shoots after a second or two.

foolproof. and safer in theory.

he even did it with polyfil, it burns the polyfil a little but worked to our amazement.




the needle was on fire?


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OfflineFlurpes
Stranger

Registered: 01/19/08
Posts: 20
Last seen: 15 years, 9 months
Re: Did I just screw up badly [Re: shevanel]
    #7992405 - 02/07/08 04:00 PM (15 years, 11 months ago)

Damn, the more I think about it the more I think I might have messed up. A bunch of little things like the cover of the clothes bin touching the floor(but I just used water to clean it off after). Me not lighting enough of the syringe( I just lit the tip and a little beyond that but not very far past that.) And me not giving the tip enough time to cool off (although I think by the time I squirted it was cooled off enough. Another thing was that I put the syringe up to the side of the glass and squirted but I didn't look at what was going on just at the top of the lid. And the last little thing I can think of was me not waiting long enough to inoculate the jars after they where steam sterilized. I waited long enough that the jars weren't hot anymore and just a little bit warm. Shit, waiting sucks so bad....I guess only time will tell if I did it right.


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OfflineNibin
Getting there
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Registered: 11/29/05
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Re: Did I just screw up badly [Re: Flurpes]
    #7992421 - 02/07/08 04:04 PM (15 years, 11 months ago)

Put them in a closet (or your incubator) and forget about them for a week.

Then look and see if you got results.


--------------------
Newcomers guide-----> For all things shroomy


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Invisibleflavoraid
now with twicethe ketamine andopiates!
Male
Registered: 12/05/07
Posts: 1,678
Re: Did I just screw up badly [Re: Nibin]
    #7992529 - 02/07/08 04:29 PM (15 years, 11 months ago)

I used a cigarette lighter, no problems. (for the most part)


--------------------
coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.


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OfflineFlurpes
Stranger

Registered: 01/19/08
Posts: 20
Last seen: 15 years, 9 months
Re: Did I just screw up badly [Re: flavoraid]
    #7992708 - 02/07/08 05:08 PM (15 years, 11 months ago)

For the most part?


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OfflineiTrip
Electronic Trip
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Registered: 11/21/07
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Last seen: 14 years, 6 months
Re: Did I just screw up badly [Re: flavoraid]
    #7992815 - 02/07/08 05:42 PM (15 years, 11 months ago)

Quote:

flavoraid said:
I used a cigarette lighter, no problems. (for the most part)




:thumbup: so have i and had no probs at all.
so dont worry about it


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OfflineNibin
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Re: Did I just screw up badly [Re: iTrip]
    #7992840 - 02/07/08 05:46 PM (15 years, 11 months ago)

Only problem I see with using a cig lighter (btw where the fuck is mine) is that it leaves a sooty residue on the needle.


--------------------
Newcomers guide-----> For all things shroomy


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Offlinebeatyou
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Registered: 02/21/06
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Loc: austin, tx
Last seen: 2 years, 5 months
Re: Did I just screw up badly [Re: Nibin]
    #7992859 - 02/07/08 05:50 PM (15 years, 11 months ago)

Quote:

Nibin said:
Only problem I see with using a cig lighter (btw where the fuck is mine) is that it leaves a sooty residue on the needle.




the needle tip is still sterile, not really a problem.


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OfflineNibin
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Re: Did I just screw up badly [Re: beatyou]
    #7992965 - 02/07/08 06:17 PM (15 years, 11 months ago)

Well, after recycling a needle 20 times it tends to build up.

But that happens to me coz I'm a cheap bugger :rotfl:


--------------------
Newcomers guide-----> For all things shroomy


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Offlinegaladar


Registered: 05/09/07
Posts: 981
Loc: in a monotub!
Last seen: 2 years, 2 months
Re: Did I just screw up badly [Re: shevanel]
    #7993724 - 02/07/08 08:24 PM (15 years, 11 months ago)

Quote:

shevanel said:
Quote:

galadar said:
Quote:

tahoe said:
you should be fine. In theory soot would have better odds of being free of contams than a alcohol wipe. I would never touch the needle after flaming it




tru, a friend of a friend just sticks the flaming hot needle into the jar(pf tek) where it is sterile and shoots after a second or two.

foolproof. and safer in theory.

he even did it with polyfil, it burns the polyfil a little but worked to our amazement.




the needle was on fire?




ummm. no..... flaming hot(flamed), not flaming.


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