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tahoe
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2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio Updated 2/15
#7984872 - 02/05/08 10:06 PM (15 years, 11 months ago) |
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SO i made 2 pf cakes old school style. Brf/verm, 1/2 pint, 1/2 inch verm layer, metal lid, four holes, and pc'ed for 20 mins.
I always see people telling other's not to use too much of their ss because it will screw up the water ratio. This might be true but how much is too much? I would guess 50cc's would be too much but would 12cc's do it? Would 23cc's do it?
So here goes. 1 cake gets a 12 cc spore syringe and the other gets 23cc's of lc.

Inoculation in action You can see the fluid

Wow fastest sign of life ever. There is already mycelium in the jar. See the blobs in the bottom jar!!

What do you guys think will happen?
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
Edited by tahoe (02/15/08 02:19 PM)
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UCX_911
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: tahoe]
#7984885 - 02/05/08 10:09 PM (15 years, 11 months ago) |
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I dont know when or if you're ever joking.
-------------------- "Reefer Madness."
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tahoe
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: UCX_911]
#7984895 - 02/05/08 10:11 PM (15 years, 11 months ago) |
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the pics do not lie. Do you think something awful is going to happen? If so, why?
Did you folks notice that there isnt a glovebox,flowhood, didnt flame the needle, didnt wear gloves?
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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enesi
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: tahoe]
#7984917 - 02/05/08 10:16 PM (15 years, 11 months ago) |
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why waste so much culture on one little brf jar?
when i use an LC to inoculate 1/2 pints, i use maybe 1/4cc of LC solution per hole.Just enough to see it come out the end of the needle, probably a drop. and it shows growth within 12-16 hours.
why waste an entire syringe on ONE jar?
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tahoe
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: enesi]
#7984923 - 02/05/08 10:17 PM (15 years, 11 months ago) |
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Quote:
enesi said: why waste so much culture on one little brf jar?
when i use an LC to inoculate 1/2 pints, i use maybe 1/4cc of LC solution per hole.Just enough to see it come out the end of the needle, probably a drop. and it shows growth within 12-16 hours.
why waste an entire syringe on ONE jar?
did you read the title and/or the post?
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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xile


Registered: 10/04/07
Posts: 63
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: tahoe]
#7984928 - 02/05/08 10:18 PM (15 years, 11 months ago) |
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If there are enough spores / lc being injected, couldn't they beat the contams in a time race? So theoretically, you would need to be safer with less CCs injected, as they take longer to spread.
If you did decide to do a huge push with.. 23ccs of mycelium, damn btw, i don't think there would be that much of a contam risk.
This is speculation and in no way fact, I don't know.
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enesi
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: tahoe]
#7984937 - 02/05/08 10:22 PM (15 years, 11 months ago) |
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Quote:
tahoe said:
Quote:
enesi said: why waste so much culture on one little brf jar?
when i use an LC to inoculate 1/2 pints, i use maybe 1/4cc of LC solution per hole.Just enough to see it come out the end of the needle, probably a drop. and it shows growth within 12-16 hours.
why waste an entire syringe on ONE jar?
did you read the title and/or the post?
yes, i did, and i don't see the point in such extreme amounts you are opting to use here.
You are comparing using one entire syringe of solution..to using TWO entire syringes of solution for a single 1/2 pint jar. Both of which are pointless. I realize what you are aiming to prove, and if you were comparing something like, 4cc's per jar to 12cc's per jar, then it would make a bit more sense.
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tahoe
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: enesi]
#7984946 - 02/05/08 10:24 PM (15 years, 11 months ago) |
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what am I aiming to prove?
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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im_on_a_boat
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: enesi]
#7984948 - 02/05/08 10:25 PM (15 years, 11 months ago) |
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prob gonna colonize fuckin fast..
hope ya get some diverse fruits with that many spores 
if you think about it this way..
you could just make a spore syringe with a shitload of spores in it instead of shooting 12cc's into each jar to throw off its water content..
that way you can shoot a more concentrated amount into the jar and possibly get the same effects..
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UCX_911
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: tahoe]
#7984949 - 02/05/08 10:25 PM (15 years, 11 months ago) |
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beats me. come on over to my thread and rate my grow!
-------------------- "Reefer Madness."
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enesi
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: tahoe]
#7984953 - 02/05/08 10:26 PM (15 years, 11 months ago) |
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Quote:
tahoe said: what am I aiming to prove?
did you even read the first post?? 
Quote:
I always see people telling other's not to use too much of their ss because it will screw up the water ratio. This might be true but how much is too much?
but whatever, continue on...
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tahoe
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: enesi]
#7984990 - 02/05/08 10:36 PM (15 years, 11 months ago) |
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Quote:
enesi said: I realize what you are aiming to prove, and if you were comparing something like, 4cc's per jar to 12cc's per jar, then it would make a bit more sense.
If 12 and 23 cc's do not screw it up then I do not think 4 cc's will screw it up, you wouldnt even have to think twice about 4 cc's
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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JewelessCaesar
Shroomery's Legal Department




Registered: 05/26/06
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: tahoe]
#7985087 - 02/05/08 11:00 PM (15 years, 11 months ago) |
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Quote:
tahoe said:
Quote:
enesi said: I realize what you are aiming to prove, and if you were comparing something like, 4cc's per jar to 12cc's per jar, then it would make a bit more sense.
If 12 and 23 cc's do not screw it up then I do not think 4 cc's will screw it up, you wouldnt even have to think twice about 4 cc's
I have also wondered about this.. continue on with the experiment my friend... I will look for updates.
Plus, I believe the jars will be fine..the only one that might have a problem is the 23ccs but if it looks good so far..hell yeah I think you've got a mythbuster..
-------------------- Failure is not an option, it's an adventure! - JewelessCaesar
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tahoe
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: JewelessCaesar]
#7985939 - 02/06/08 09:20 AM (15 years, 11 months ago) |
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update: both jars have completely absorb the excess water. The both look a little wet but not anything to worry about. They are not even remotely looking like a jar that has gotten water in it during boiling.
The lc is changing to mycelium. The ss is still just looking the same.
Please remember that in general it is a waste to use more than a 1/4-1/2cc of a ss per hole on a brf/verm jar. with lc's you can pretty much use as much as you want but there will be a point at which it is too much and water content will be fucked.
EDIT: Damn My spelling sucks in the morning
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
Edited by tahoe (02/06/08 09:31 AM)
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large_dose
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: tahoe]
#7985973 - 02/06/08 09:27 AM (15 years, 11 months ago) |
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interesting.
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shroober
Myco Junkie

Registered: 01/02/08
Posts: 879
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: tahoe]
#7985975 - 02/06/08 09:28 AM (15 years, 11 months ago) |
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nice experiment, its good to see these ridiculous, overly cautious myths proven to be absolutely untrue, i think i read somewhere here that anything over 2cc would fuck up a jar's water content, its good to see someone prove this shit wrong
im planning on doing an "everything wrong" grow pretty soon. im not going to be sterile, im going to pretty much fuck up everything i can using the PF tek and see what type of results i get, should be fun
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tahoe
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: shroober]
#7985992 - 02/06/08 09:33 AM (15 years, 11 months ago) |
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just remember that what I am doing isn't exactly wrong but it isn't good. This is not the right way to do it. But hopefully some noob will see this when he thinks he fucked up because he used 1 cc per hole and will not post a repetitive post because of it.
Okay I am out. I am going skiing!
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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enesi
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: tahoe]
#7986954 - 02/06/08 02:19 PM (15 years, 11 months ago) |
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so if your jar stalls or contams, how are you going to know what caused it?? You used no sterile procedures, and you have no control for the experiment either. So if it doesn't work, you wont be able to say why. Not using sterile procedures kind of flawed the entire thing from the get go.
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primal21
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: enesi]
#7987514 - 02/06/08 04:27 PM (15 years, 11 months ago) |
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Yeah but if it does work
MYTH BUSTED
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atomicblue
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: tahoe]
#7987604 - 02/06/08 04:49 PM (15 years, 11 months ago) |
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I just did the same with some Hammond Azurescens syringes . I started some Ossip Azures about a month ago and wanted the Hammonds to catch up so I can be ready for spring. The Ossip jars we're inoculated at four points with just a drop or two of solution and these took about a month to completely colonize. The Hammonds we're inoculated about a week ago with eight inoculation points and extra spore water added at each injection site. They are 50% colonized already! I made a syringe off of a print then injected the syringe into a half pint jar of sterile water to extend my syringe out further.This gave me 10 syringes out of the original one. I used one of those to do the heavily inoculated Hammond jars and they still grew way faster and stronger with the extra water.:)
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atomicblue
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: atomicblue]
#7987623 - 02/06/08 04:54 PM (15 years, 11 months ago) |
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You can probably drown your jars out to the point of some kind of contamination but mine have worked out great. I have lost one jar out of 50 or so. Sterility is not needed during inoculation in the PF Tek. Inoculation can be done out in the open as long as your syringes we're made in a glove box and you flame em between each jar. Thanks Tahoe,cool test!
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tahoe
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: enesi]
#7988302 - 02/06/08 07:04 PM (15 years, 11 months ago) |
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Quote:
enesi said: so if your jar stalls or contams, how are you going to know what caused it?? You used no sterile procedures, .
That is the second part of this project. Sterile techniuqe isnt needed for the pf tek. Inoculation of the Pf tek is meant to be done just the way i did.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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tahoe
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: tahoe]
#7991038 - 02/07/08 10:23 AM (15 years, 11 months ago) |
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2 days and another pic. the lc is showing real signs of life as expected

Although I am worried about this one
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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blood4blood
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: tahoe]
#7991095 - 02/07/08 10:42 AM (15 years, 11 months ago) |
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the ss one is looking terrible! i would def throw that against a wall of some abandoned building late at night.
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tahoe
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: blood4blood]
#7991185 - 02/07/08 11:02 AM (15 years, 11 months ago) |
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hah, no the syringe one is in the top pic but on the bottom. The bottom pic is a differnt strain/cake. it is oyster on black wood chips. If it were cubes it would be trashed but it is fine
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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dbt123
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio Updated 2/7 [Re: tahoe]
#7991546 - 02/07/08 12:42 PM (15 years, 11 months ago) |
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i did this with WBS jars...i injected one jar which the seed had been boiled with an about 10cc's of LC and one with wbs that hadnt been boiled with the same amount.....it took the one which hadnt been boiled about twice as long to colonize.....
in the case of WBS i beleive it is the prep work that really determines the colonizing speed....in the case of BRF cakes i think you could say the same thing....though you cant over look the fact that the same cake with more LC will probably colonize faster just because there are more points for the myc to start from....take two BRF cake one with very fine verm an one with very corse verm an see which one colonizes faster...prep work is key
great experiment though, i really dont think too much LC will screw it up as long as ur steril....the same amount of contams will go in with 2cc or 10cc(hopefully none)....though i dont think creating a BRF float will do you any good:)
-D
-------------------- Anything said by DBT123 is a complete act of fiction and is in no way linked to any person....:)
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tahoe
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio Updated 2/7 [Re: dbt123]
#8006184 - 02/10/08 07:44 PM (15 years, 11 months ago) |
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the lc jar is almost fully colonized. The ss jar is showing signs of germination and growth. Water content does not seem to screwed up on either.
The ss was the syringe that I made from the thinning tech that I posted a week or so ago
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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atomicblue
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio Updated 2/7 [Re: tahoe]
#8007241 - 02/11/08 12:19 AM (15 years, 11 months ago) |
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Same here, my Mazzies are cruising fast on the thinned out ss as are my Hammonds passing up some Ossips started a month ago. This took two weeks out of the equation.:) A little extra even thinned out seems to make jars cultivate much quicker.
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tahoe
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio Updated 2/7 [Re: atomicblue]
#8008283 - 02/11/08 11:06 AM (15 years, 11 months ago) |
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Here they are today.
 By the way, the syringe used to inoculate the spore syringe jars is the same one that I made in this tech. Notice that it was made without a glovebox and was used without a glove box..
http://www.shroomery.org/forums/showflat.php/Number/7962205#7962205
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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tahoe
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio Updated 2/7 [Re: tahoe]
#8027559 - 02/15/08 02:18 PM (15 years, 11 months ago) |
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here they are today. The lc is almost fully colonized No sign of a screwed up water content. Sorry about the sideways pic
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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blood4blood
Calmer Than You Are


Registered: 04/25/07
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio Updated 2/7 [Re: tahoe]
#8027719 - 02/15/08 03:05 PM (15 years, 11 months ago) |
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impressive man....
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smokedout420
The EnlightenedOne



Registered: 12/16/07
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio Updated 2/7 [Re: blood4blood]
#8028052 - 02/15/08 04:29 PM (15 years, 11 months ago) |
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smily
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio Updated 2/7 [Re: smokedout420]
#8028161 - 02/15/08 04:59 PM (15 years, 11 months ago) |
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cant wait to see the jar look like those you got shown it wont be long
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robanero
β Lib


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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio Updated 2/7 [Re: smily]
#8314232 - 04/22/08 12:23 PM (15 years, 9 months ago) |
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I don't think Tahoe can screw ANY grow up.
How long have you been growing Tahoe? I know the answer is here somewhere but I can't find it.
--------------------
  310 open cap Hawaiians grown a 1 pint WBSF cake. SPAWN RATIO CALCULATOR My Little Hawaiians Spawn Bag Tek
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shroober
Myco Junkie


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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio Updated 2/7 [Re: robanero]
#8314297 - 04/22/08 12:40 PM (15 years, 9 months ago) |
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Quote:
robanero said: I don't think Tahoe can screw ANY grow up.
How long have you been growing Tahoe? I know the answer is here somewhere but I can't find it.
i think i just threw up a little in my mouth
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robanero
β Lib


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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio Updated 2/7 [Re: shroober]
#8314434 - 04/22/08 01:26 PM (15 years, 9 months ago) |
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Quote:
shroober said:
Quote:
robanero said: I don't think Tahoe can screw ANY grow up.
How long have you been growing Tahoe? I know the answer is here somewhere but I can't find it.
i think i just threw up a little in my mouth
That's some funny Shit!!
--------------------
  310 open cap Hawaiians grown a 1 pint WBSF cake. SPAWN RATIO CALCULATOR My Little Hawaiians Spawn Bag Tek
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prototypical_man
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio Updated 2/7 [Re: robanero]
#8314969 - 04/22/08 03:51 PM (15 years, 9 months ago) |
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What was the total colonization time for the LC?
-------------------- I have 29 Strains as of current.. I LOVE TO TRADE. See my bio for the strains I currently have. I seek: Strains I don't have, exotic species, Edibles, Vacutainers, A accurate scale, chicken manure, worm castings, worms for making my own worm castings, plastic lids for mason jars, light malt extract, Kelp Meal, A Ph Tester, A microscope.
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