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Offlineatomicblue
Stranger
Female


Registered: 11/09/07
Posts: 206
Loc: UP
Last seen: 14 years, 2 months
Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: atomicblue]
    #7987623 - 02/06/08 04:54 PM (15 years, 11 months ago)

You can probably drown your jars out to the point of some kind of contamination but mine have worked out great. I have lost one jar out of 50 or so. Sterility is not needed during inoculation in the PF Tek. Inoculation can be done out in the open as long as your syringes we're made in a glove box and you flame em between each jar. Thanks Tahoe,cool test!


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Invisibletahoe
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Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
Trusted Cultivator
Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: enesi]
    #7988302 - 02/06/08 07:04 PM (15 years, 11 months ago)

Quote:

enesi said:
so if your jar stalls or contams, how are you going to know what caused it?? You used no sterile procedures, .




That is the second part of this project. Sterile techniuqe isnt needed for the pf tek. Inoculation of the Pf tek is meant to be done just the way i did.


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Invisibletahoe
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Registered: 11/26/03
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: tahoe]
    #7991038 - 02/07/08 10:23 AM (15 years, 11 months ago)

2 days and another pic.
the lc is showing real signs of life as expected


Although I am worried about this one


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Invisibleblood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: tahoe]
    #7991095 - 02/07/08 10:42 AM (15 years, 11 months ago)

the ss one is looking terrible! i would def throw that against a wall of some abandoned building late at night.


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Invisibletahoe
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: blood4blood]
    #7991185 - 02/07/08 11:02 AM (15 years, 11 months ago)

hah, no the syringe one is in the top pic but on the bottom.
The bottom pic is a differnt strain/cake. it is oyster on black wood chips. If it were cubes it would be trashed but it is fine


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Offlinedbt123
Stranger

Registered: 12/10/07
Posts: 54
Last seen: 14 years, 5 days
Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio Updated 2/7 [Re: tahoe]
    #7991546 - 02/07/08 12:42 PM (15 years, 11 months ago)

i did this with WBS jars...i injected one jar which the seed had been boiled with an about 10cc's of LC and one with wbs that hadnt been boiled with the same amount.....it took the one which hadnt been boiled about twice as long to colonize.....

in the case of WBS i beleive it is the prep work that really determines the colonizing speed....in the case of BRF cakes i think you could say the same thing....though you cant over look the fact that the same cake with more LC will probably colonize faster just because there are more points for the myc to start from....take two BRF cake one with very fine verm an one with very corse verm an see which one colonizes faster...prep work is key

great experiment though, i really dont think too much LC will screw it up as long as ur steril....the same amount of contams will go in with 2cc or 10cc(hopefully none)....though i dont think creating a BRF float will do you any good:)

-D


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Anything said by DBT123 is a complete act of fiction and is in no way linked to any person....:)


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Invisibletahoe
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio Updated 2/7 [Re: dbt123]
    #8006184 - 02/10/08 07:44 PM (15 years, 11 months ago)

the lc jar is almost fully colonized. The ss jar is showing signs of germination and growth. Water content does not seem to screwed up on either.

The ss was the syringe that I made from the thinning tech that I posted a week or so ago


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Offlineatomicblue
Stranger
Female


Registered: 11/09/07
Posts: 206
Loc: UP
Last seen: 14 years, 2 months
Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio Updated 2/7 [Re: tahoe]
    #8007241 - 02/11/08 12:19 AM (15 years, 11 months ago)

Same here, my Mazzies are cruising fast on the thinned out ss as are my Hammonds passing up some Ossips started a month ago. This took two weeks out of the equation.:) A little extra even thinned out seems to make jars cultivate much quicker.


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Invisibletahoe
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio Updated 2/7 [Re: atomicblue]
    #8008283 - 02/11/08 11:06 AM (15 years, 11 months ago)

Here they are today.

By the way, the syringe used to inoculate the spore syringe jars is the same one that I made in this tech. Notice that it was made without a glovebox and was used without a glove box..

http://www.shroomery.org/forums/showflat.php/Number/7962205#7962205


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Invisibletahoe
Noob Slayer
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Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
Trusted Cultivator
Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio Updated 2/7 [Re: tahoe]
    #8027559 - 02/15/08 02:18 PM (15 years, 11 months ago)

here they are today. The lc is almost fully colonized
No sign of a screwed up water content. Sorry about the sideways pic


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Invisibleblood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio Updated 2/7 [Re: tahoe]
    #8027719 - 02/15/08 03:05 PM (15 years, 11 months ago)

impressive man....


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Invisiblesmokedout420
The EnlightenedOne
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Registered: 12/16/07
Posts: 484
Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio Updated 2/7 [Re: blood4blood]
    #8028052 - 02/15/08 04:29 PM (15 years, 11 months ago)

:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:
thanks for the info:thumbup:


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Offlinesmily
lookin 4 my ass wit both handz
I'm a teapot User Gallery


Registered: 07/13/06
Posts: 2,592
Loc: Lee HO FooKs
Last seen: 9 months, 3 days
Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio Updated 2/7 [Re: smokedout420]
    #8028161 - 02/15/08 04:59 PM (15 years, 11 months ago)

cant wait to see the jar look like those you got shown it wont be long


:smile:


--------------------


    CrAnKy PiLlOwS YeAh PiLlOwS


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Offlinerobanero
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio Updated 2/7 [Re: smily]
    #8314232 - 04/22/08 12:23 PM (15 years, 9 months ago)

I don't think Tahoe can screw ANY grow up.

How long have you been growing Tahoe? I know the answer is here somewhere but I can't find it.


--------------------

310 open cap Hawaiians grown a 1 pint WBSF cake.

SPAWN RATIO CALCULATOR


My Little Hawaiians

Spawn Bag Tek


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Invisibleshroober
Myco Junkie


Registered: 01/02/08
Posts: 879
Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio Updated 2/7 [Re: robanero]
    #8314297 - 04/22/08 12:40 PM (15 years, 9 months ago)

Quote:

robanero said:
I don't think Tahoe can screw ANY grow up.

How long have you been growing Tahoe? I know the answer is here somewhere but I can't find it.




i think i just threw up a little in my mouth


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Offlinerobanero
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Posts: 874
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio Updated 2/7 [Re: shroober]
    #8314434 - 04/22/08 01:26 PM (15 years, 9 months ago)

Quote:

shroober said:
Quote:

robanero said:
I don't think Tahoe can screw ANY grow up.

How long have you been growing Tahoe? I know the answer is here somewhere but I can't find it.




i think i just threw up a little in my mouth





That's some funny Shit!!


--------------------

310 open cap Hawaiians grown a 1 pint WBSF cake.

SPAWN RATIO CALCULATOR


My Little Hawaiians

Spawn Bag Tek


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Offlineprototypical_man
ass model


Registered: 02/01/05
Posts: 396
Loc: Doral Florida
Last seen: 7 years, 11 months
Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio Updated 2/7 [Re: robanero]
    #8314969 - 04/22/08 03:51 PM (15 years, 9 months ago)

What was the total colonization time for the LC?


--------------------
I have 29 Strains as of current.. I LOVE TO TRADE. See my bio for the strains I currently have.

I seek: Strains I don't have, exotic species, Edibles, Vacutainers, A accurate scale, chicken manure, worm castings, worms for making my own worm castings, plastic lids for mason jars, light malt extract, Kelp Meal, A Ph Tester, A microscope.


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