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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio Updated 2/15
#7984872 - 02/05/08 10:06 PM (15 years, 11 months ago) |
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SO i made 2 pf cakes old school style. Brf/verm, 1/2 pint, 1/2 inch verm layer, metal lid, four holes, and pc'ed for 20 mins.
I always see people telling other's not to use too much of their ss because it will screw up the water ratio. This might be true but how much is too much? I would guess 50cc's would be too much but would 12cc's do it? Would 23cc's do it?
So here goes. 1 cake gets a 12 cc spore syringe and the other gets 23cc's of lc.

Inoculation in action You can see the fluid

Wow fastest sign of life ever. There is already mycelium in the jar. See the blobs in the bottom jar!!

What do you guys think will happen?
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
Edited by tahoe (02/15/08 02:19 PM)
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UCX_911
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: tahoe]
#7984885 - 02/05/08 10:09 PM (15 years, 11 months ago) |
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I dont know when or if you're ever joking.
-------------------- "Reefer Madness."
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: UCX_911]
#7984895 - 02/05/08 10:11 PM (15 years, 11 months ago) |
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the pics do not lie. Do you think something awful is going to happen? If so, why?
Did you folks notice that there isnt a glovebox,flowhood, didnt flame the needle, didnt wear gloves?
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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enesi
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: tahoe]
#7984917 - 02/05/08 10:16 PM (15 years, 11 months ago) |
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why waste so much culture on one little brf jar?
when i use an LC to inoculate 1/2 pints, i use maybe 1/4cc of LC solution per hole.Just enough to see it come out the end of the needle, probably a drop. and it shows growth within 12-16 hours.
why waste an entire syringe on ONE jar?
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: enesi]
#7984923 - 02/05/08 10:17 PM (15 years, 11 months ago) |
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Quote:
enesi said: why waste so much culture on one little brf jar?
when i use an LC to inoculate 1/2 pints, i use maybe 1/4cc of LC solution per hole.Just enough to see it come out the end of the needle, probably a drop. and it shows growth within 12-16 hours.
why waste an entire syringe on ONE jar?
did you read the title and/or the post?
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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xile


Registered: 10/04/07
Posts: 63
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: tahoe]
#7984928 - 02/05/08 10:18 PM (15 years, 11 months ago) |
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If there are enough spores / lc being injected, couldn't they beat the contams in a time race? So theoretically, you would need to be safer with less CCs injected, as they take longer to spread.
If you did decide to do a huge push with.. 23ccs of mycelium, damn btw, i don't think there would be that much of a contam risk.
This is speculation and in no way fact, I don't know.
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enesi
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: tahoe]
#7984937 - 02/05/08 10:22 PM (15 years, 11 months ago) |
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Quote:
tahoe said:
Quote:
enesi said: why waste so much culture on one little brf jar?
when i use an LC to inoculate 1/2 pints, i use maybe 1/4cc of LC solution per hole.Just enough to see it come out the end of the needle, probably a drop. and it shows growth within 12-16 hours.
why waste an entire syringe on ONE jar?
did you read the title and/or the post?
yes, i did, and i don't see the point in such extreme amounts you are opting to use here.
You are comparing using one entire syringe of solution..to using TWO entire syringes of solution for a single 1/2 pint jar. Both of which are pointless. I realize what you are aiming to prove, and if you were comparing something like, 4cc's per jar to 12cc's per jar, then it would make a bit more sense.
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tahoe
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: enesi]
#7984946 - 02/05/08 10:24 PM (15 years, 11 months ago) |
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what am I aiming to prove?
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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im_on_a_boat
Stranger

Registered: 04/06/06
Posts: 3,950
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: enesi]
#7984948 - 02/05/08 10:25 PM (15 years, 11 months ago) |
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prob gonna colonize fuckin fast..
hope ya get some diverse fruits with that many spores 
if you think about it this way..
you could just make a spore syringe with a shitload of spores in it instead of shooting 12cc's into each jar to throw off its water content..
that way you can shoot a more concentrated amount into the jar and possibly get the same effects..
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UCX_911
VideoSlave


Registered: 01/13/08
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: tahoe]
#7984949 - 02/05/08 10:25 PM (15 years, 11 months ago) |
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beats me. come on over to my thread and rate my grow!
-------------------- "Reefer Madness."
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enesi
On the Bus



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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: tahoe]
#7984953 - 02/05/08 10:26 PM (15 years, 11 months ago) |
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Quote:
tahoe said: what am I aiming to prove?
did you even read the first post?? 
Quote:
I always see people telling other's not to use too much of their ss because it will screw up the water ratio. This might be true but how much is too much?
but whatever, continue on...
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: enesi]
#7984990 - 02/05/08 10:36 PM (15 years, 11 months ago) |
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Quote:
enesi said: I realize what you are aiming to prove, and if you were comparing something like, 4cc's per jar to 12cc's per jar, then it would make a bit more sense.
If 12 and 23 cc's do not screw it up then I do not think 4 cc's will screw it up, you wouldnt even have to think twice about 4 cc's
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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JewelessCaesar
Shroomery's Legal Department




Registered: 05/26/06
Posts: 599
Loc: Petros-Joyner, TN, USA
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: tahoe]
#7985087 - 02/05/08 11:00 PM (15 years, 11 months ago) |
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Quote:
tahoe said:
Quote:
enesi said: I realize what you are aiming to prove, and if you were comparing something like, 4cc's per jar to 12cc's per jar, then it would make a bit more sense.
If 12 and 23 cc's do not screw it up then I do not think 4 cc's will screw it up, you wouldnt even have to think twice about 4 cc's
I have also wondered about this.. continue on with the experiment my friend... I will look for updates.
Plus, I believe the jars will be fine..the only one that might have a problem is the 23ccs but if it looks good so far..hell yeah I think you've got a mythbuster..
-------------------- Failure is not an option, it's an adventure! - JewelessCaesar
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tahoe
Noob Slayer



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Posts: 6,274
Loc: N38.93829W119.98108
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: JewelessCaesar]
#7985939 - 02/06/08 09:20 AM (15 years, 11 months ago) |
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update: both jars have completely absorb the excess water. The both look a little wet but not anything to worry about. They are not even remotely looking like a jar that has gotten water in it during boiling.
The lc is changing to mycelium. The ss is still just looking the same.
Please remember that in general it is a waste to use more than a 1/4-1/2cc of a ss per hole on a brf/verm jar. with lc's you can pretty much use as much as you want but there will be a point at which it is too much and water content will be fucked.
EDIT: Damn My spelling sucks in the morning
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
Edited by tahoe (02/06/08 09:31 AM)
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large_dose
Melonhead



Registered: 05/05/06
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Loc: Right in the Middle
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: tahoe]
#7985973 - 02/06/08 09:27 AM (15 years, 11 months ago) |
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interesting.
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shroober
Myco Junkie

Registered: 01/02/08
Posts: 879
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: tahoe]
#7985975 - 02/06/08 09:28 AM (15 years, 11 months ago) |
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nice experiment, its good to see these ridiculous, overly cautious myths proven to be absolutely untrue, i think i read somewhere here that anything over 2cc would fuck up a jar's water content, its good to see someone prove this shit wrong
im planning on doing an "everything wrong" grow pretty soon. im not going to be sterile, im going to pretty much fuck up everything i can using the PF tek and see what type of results i get, should be fun
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: shroober]
#7985992 - 02/06/08 09:33 AM (15 years, 11 months ago) |
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just remember that what I am doing isn't exactly wrong but it isn't good. This is not the right way to do it. But hopefully some noob will see this when he thinks he fucked up because he used 1 cc per hole and will not post a repetitive post because of it.
Okay I am out. I am going skiing!
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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enesi
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: tahoe]
#7986954 - 02/06/08 02:19 PM (15 years, 11 months ago) |
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so if your jar stalls or contams, how are you going to know what caused it?? You used no sterile procedures, and you have no control for the experiment either. So if it doesn't work, you wont be able to say why. Not using sterile procedures kind of flawed the entire thing from the get go.
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primal21
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: enesi]
#7987514 - 02/06/08 04:27 PM (15 years, 11 months ago) |
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Yeah but if it does work
MYTH BUSTED
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atomicblue
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Re: 2 Syringes and 2 Cakes!!! I am Trying to Screw Up the Water Ratio [Re: tahoe]
#7987604 - 02/06/08 04:49 PM (15 years, 11 months ago) |
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I just did the same with some Hammond Azurescens syringes . I started some Ossip Azures about a month ago and wanted the Hammonds to catch up so I can be ready for spring. The Ossip jars we're inoculated at four points with just a drop or two of solution and these took about a month to completely colonize. The Hammonds we're inoculated about a week ago with eight inoculation points and extra spore water added at each injection site. They are 50% colonized already! I made a syringe off of a print then injected the syringe into a half pint jar of sterile water to extend my syringe out further.This gave me 10 syringes out of the original one. I used one of those to do the heavily inoculated Hammond jars and they still grew way faster and stronger with the extra water.:)
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