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OfflineIzord
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Registered: 02/05/08
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Last seen: 14 years, 4 months
Problems with rye grain moisture content or Contaminants?
    #7983397 - 02/05/08 05:01 PM (15 years, 11 months ago)

Problems with rye grain moisture content or Contaminants?

Try this simple tek. For quart jars: add one cup (250 ml) rye, 230-240 ml water, seal with a ring and a filter disk. PC for one hour. Then when the PC is cool, shake up the jars but put them back in the PC with some water in the PC for one more PC. Next day, after the endospores have germinated (like 12-48 hours later), PC again for one hour. Then when cool shake the jars again.

inoculate with a 25 gauge needle right through the filter disk. Don't worry about the needle hole, it will self seal.

You will have perfectly hydrated rye with all endospores sterilized.

No soaking, no simmering, no rinsing.

Izord


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OfflineCiv
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Registered: 10/14/04
Posts: 2,537
Loc: California
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Re: Problems with rye grain moisture content or Contaminants? [Re: Izord]
    #7983547 - 02/05/08 05:32 PM (15 years, 11 months ago)

Quote:

Izord said:

rye with all endospores sterilized.

Izord




If that were only true! :smile:  Even after you PC your never gonna kill all the spores in grain.  Your just createing a window for what you want to grow.

No reason not to rinse either- you can rid yourself of lots of bacteria that way.  Nothing wrong with washing your grains.  You will get better results.  Especially when your skipping a simmer. (simmering can be a bit much, I agree.  I prefer to steep at the least though)

Everything else you got down this is pretty groovy.  Keep it up.


--------------------
"...Gal's seem to hate the thought of blending chicken shit in a blender.
So, wash it well afterwards & DON'T tell them..."  -Agar


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OfflineIzord
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Re: Problems with rye grain moisture content or Contaminants? [Re: Civ]
    #7983620 - 02/05/08 05:47 PM (15 years, 11 months ago)

Thanks, I just can't do too many steps, Must keep it simple. Due to poor attention span.

And I tried the soak and simmer last month and got the water content all wrong. So this time I went back to basics. Water+grain+PC. I just added the second PC when I learned about endospores, which I had never heard of before. But I read about them here, and looked them up on wikipedia. Evidently they can survive one PC. But then they germinate and lose their resistant coating and can be killed with the second PC. It takes about 8 hours in a non-reassuring environment for a bacteria to create an endospore. So I just keep the jars in my grow room at about 80-85,and humid, and the bacterial endospores think everything is ok to germinate, then, bam, I hit them with another hour of 15psi and heat. Buh-bye bacteria.

The typical recipe is 250 ml rye and 220 water, I just add a little water to compensate for what evaporates during the second PC cycle. T

It used to work for me back in the 80's with just grain, water and one PC! Back then spore syringes weren't readily available and I had to order prints and grow on agar, and inoculate from agar to grain. I didn't rinse then either and I had a few contaminants, but less than say 5-10%.

Also filter disks weren't available then, and they are great for some gas exchange, and shooting the innoculant through the disk with a fine needle is something I thought of this time. So I never have to expose the sterile moist grain to the air. I use a 25 gauge, 5/8 inch needle.


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