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Invisibletahoe
Noob Slayer
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Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
Trusted Cultivator
Throwing Away Good Cultures
    #7982632 - 02/05/08 01:10 PM (15 years, 11 months ago)

Do you guys feel bad when you throw out a prefectly good culture??
I have a few cultures that are good/great performers on agar but my stockpile is geting high and I have some great performers that will be used so i will trash these other ones. I always feel bad trashing the live mycelium without giving it a chance.
I am usually the first one to bitch about shiity pics but those of you who have taken pics of the petris know my pain. I didnt want to deal with changing the lighting but I think you will get the point

these are going into the trash. You can see a quarter sized circle in each dish. This was due to temp. They were cold for a wek so i put them somewhere else and they took off



Being replaced my these


this one looks great, still sectoring a bit but very aggressive


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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InvisibleRoadkillM
Retired Shroomery Mod
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Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
Re: Throwing Away Good Cultures [Re: tahoe]
    #7982652 - 02/05/08 01:16 PM (15 years, 11 months ago)

yeah I feel bad when I throw out perfectly good cultures...

so some times I will cut the agar up into little wedges and put them into some large test tubes that I have...

then put them into the frig for use later.


I use a black background sometimes when taking pictures of petri dishes...



and they seem to come out clearer looking...at least to me they do


tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlineshevanel
Gone til November
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Registered: 01/27/08
Posts: 1,517
Last seen: 1 year, 11 months
Re: Throwing Away Good Cultures [Re: Roadkill]
    #7982669 - 02/05/08 01:21 PM (15 years, 11 months ago)

How long can a culture in the petri dish that's 100% colonized be stored, and how do you store it?


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Invisibletahoe
Noob Slayer
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Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: Throwing Away Good Cultures [Re: Roadkill]
    #7982673 - 02/05/08 01:22 PM (15 years, 11 months ago)

my desk is black but it is gloss black. I will get a matte black piece of paper for the future. I like the shots with the light on the backside.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Invisibletahoe
Noob Slayer
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Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: Throwing Away Good Cultures [Re: tahoe]
    #7982686 - 02/05/08 01:24 PM (15 years, 11 months ago)

store it in the firdge. Years, culture slants work good also. They have more moisture per surface space. I had a 2 year old culture that was left out and it dried up. I was able to cut it and still use it.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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InvisibleTomandjerry58
Stranger
Registered: 01/27/03
Posts: 5,212
Re: Throwing Away Good Cultures [Re: tahoe]
    #7982695 - 02/05/08 01:26 PM (15 years, 11 months ago)

thats one thing you always run into with cultivation.. you find youself having too much. spores... mycelium. too much workload if you carry out the whole process.


store them in the fridge ..always fantasized about having a dedicated fridge for my adventures


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Invisibleim_on_a_boat
Stranger

Registered: 04/06/06
Posts: 3,950
Re: Throwing Away Good Cultures [Re: tahoe]
    #7982722 - 02/05/08 01:31 PM (15 years, 11 months ago)

throw them into an outdoor grow or compost.. :laugh:

then you wont feel so bad..


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Offlinesomebody041
bud tester
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Registered: 06/04/07
Posts: 476
Loc: California
Last seen: 9 years, 9 months
Re: Throwing Away Good Cultures [Re: im_on_a_boat]
    #7982976 - 02/05/08 02:58 PM (15 years, 11 months ago)

tahoe, in your last pic of the very aggressive petri, which section would you choose to transfer?


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Invisibletahoe
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Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: Throwing Away Good Cultures [Re: somebody041]
    #7983135 - 02/05/08 03:47 PM (15 years, 11 months ago)

its tough. The bottom is the thickest, the bottom and left are the fastest. They all look good. That is transfered from the spore master.
I would expect a little more sectoring but the master looks awesome also

here is the master. This is from a print from the offspring of a wild mushroom that I cloned and I have growing on wood chips. It was a badass specimen and its spore produce aggressive mycelium without much isolation.you can see where I cut it from.
Check out the sector in green racing around and coming from nowhere.
I plan on transfering part of the red section to another dish and to a lc later today.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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