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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Throwing Away Good Cultures
#7982632 - 02/05/08 01:10 PM (15 years, 11 months ago) |
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Do you guys feel bad when you throw out a prefectly good culture?? I have a few cultures that are good/great performers on agar but my stockpile is geting high and I have some great performers that will be used so i will trash these other ones. I always feel bad trashing the live mycelium without giving it a chance. I am usually the first one to bitch about shiity pics but those of you who have taken pics of the petris know my pain. I didnt want to deal with changing the lighting but I think you will get the point
these are going into the trash. You can see a quarter sized circle in each dish. This was due to temp. They were cold for a wek so i put them somewhere else and they took off

Being replaced my these

this one looks great, still sectoring a bit but very aggressive
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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Roadkill
Retired Shroomery Mod



Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Throwing Away Good Cultures [Re: tahoe]
#7982652 - 02/05/08 01:16 PM (15 years, 11 months ago) |
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yeah I feel bad when I throw out perfectly good cultures...
so some times I will cut the agar up into little wedges and put them into some large test tubes that I have...
then put them into the frig for use later.
I use a black background sometimes when taking pictures of petri dishes...

and they seem to come out clearer looking...at least to me they do
tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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shevanel
Gone til November



Registered: 01/27/08
Posts: 1,517
Last seen: 1 year, 11 months
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Re: Throwing Away Good Cultures [Re: Roadkill]
#7982669 - 02/05/08 01:21 PM (15 years, 11 months ago) |
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How long can a culture in the petri dish that's 100% colonized be stored, and how do you store it?
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: Throwing Away Good Cultures [Re: Roadkill]
#7982673 - 02/05/08 01:22 PM (15 years, 11 months ago) |
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my desk is black but it is gloss black. I will get a matte black piece of paper for the future. I like the shots with the light on the backside.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: Throwing Away Good Cultures [Re: tahoe]
#7982686 - 02/05/08 01:24 PM (15 years, 11 months ago) |
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store it in the firdge. Years, culture slants work good also. They have more moisture per surface space. I had a 2 year old culture that was left out and it dried up. I was able to cut it and still use it.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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Tomandjerry58
Stranger
Registered: 01/27/03
Posts: 5,212
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Re: Throwing Away Good Cultures [Re: tahoe]
#7982695 - 02/05/08 01:26 PM (15 years, 11 months ago) |
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thats one thing you always run into with cultivation.. you find youself having too much. spores... mycelium. too much workload if you carry out the whole process.
store them in the fridge ..always fantasized about having a dedicated fridge for my adventures
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im_on_a_boat
Stranger

Registered: 04/06/06
Posts: 3,950
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Re: Throwing Away Good Cultures [Re: tahoe]
#7982722 - 02/05/08 01:31 PM (15 years, 11 months ago) |
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throw them into an outdoor grow or compost.. 
then you wont feel so bad..
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somebody041
bud tester


Registered: 06/04/07
Posts: 476
Loc: California
Last seen: 9 years, 9 months
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Re: Throwing Away Good Cultures [Re: im_on_a_boat]
#7982976 - 02/05/08 02:58 PM (15 years, 11 months ago) |
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tahoe, in your last pic of the very aggressive petri, which section would you choose to transfer?
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: Throwing Away Good Cultures [Re: somebody041]
#7983135 - 02/05/08 03:47 PM (15 years, 11 months ago) |
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its tough. The bottom is the thickest, the bottom and left are the fastest. They all look good. That is transfered from the spore master. I would expect a little more sectoring but the master looks awesome also
here is the master. This is from a print from the offspring of a wild mushroom that I cloned and I have growing on wood chips. It was a badass specimen and its spore produce aggressive mycelium without much isolation.you can see where I cut it from. Check out the sector in green racing around and coming from nowhere. I plan on transfering part of the red section to another dish and to a lc later today.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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