|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
usagolden
A Tin Can Banana
Registered: 02/04/08
Posts: 78
Last seen: 6 years, 1 month
|
premix culture powder--need help!
#7978535 - 02/04/08 04:54 PM (16 years, 15 days ago) |
|
|
i bought a big bottle of premix dextrose/potato/agar mix off ebay. i followed the directions on the bottle and made the agar, which was 39g in one liter of water, boil to dissolve and autoclave at 15psi for 20 min. when they came out of the pressure cooker the agar solution was hard and jelly-like; i can't mix it up no matter how much i shake. so here is my question: how many grams should i use per liter of water?
|
RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 21 days
|
Re: premix culture powder--need help! [Re: usagolden]
#7978613 - 02/04/08 05:04 PM (16 years, 15 days ago) |
|
|
20 grams of agar agar per liter. You're supposed to pour your petri dishes before the agar cools enough to solidify. You can often re-heat it to get it liquid again. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
|
MYSTIQUE
Say Hi to the elves for me.




Registered: 04/28/07
Posts: 1,764
Loc: Canada
Last seen: 5 years, 4 months
|
Re: premix culture powder--need help! [Re: usagolden]
#7978618 - 02/04/08 05:05 PM (16 years, 15 days ago) |
|
|
I'm not pro with agar but is it still hot? You have to double boil the agar I believe to get it to go liquid then you can pour it.
-------------------- Dont know what the fuck I just said? READ THIS http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary I ain't a hippy but I'm covered in dirt Sippin lots of mushroom tea in a tye-dye shirt Chasin' the Grateful Dead, no shoes on my feet Beggin' in the parking lot for something to eat, DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!
|
usagolden
A Tin Can Banana
Registered: 02/04/08
Posts: 78
Last seen: 6 years, 1 month
|
Re: premix culture powder--need help! [Re: RogerRabbit]
#7978664 - 02/04/08 05:12 PM (16 years, 15 days ago) |
|
|
i was planning on doing some jars, not dishes. is this still possible with the potato/dextrose/agar?
|
MYSTIQUE
Say Hi to the elves for me.




Registered: 04/28/07
Posts: 1,764
Loc: Canada
Last seen: 5 years, 4 months
|
Re: premix culture powder--need help! [Re: usagolden]
#7978675 - 02/04/08 05:14 PM (16 years, 15 days ago) |
|
|
Are you trying to make agar or a l.c.? Tell us what you are planing. If you warm it up ya You can pour it into a jar and let it harden again.
-------------------- Dont know what the fuck I just said? READ THIS http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary I ain't a hippy but I'm covered in dirt Sippin lots of mushroom tea in a tye-dye shirt Chasin' the Grateful Dead, no shoes on my feet Beggin' in the parking lot for something to eat, DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 726 topic views. 10 members, 98 guests and 44 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|