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Left Coast Kratom Shop: Kratom Powder For Sale

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Offlineusagolden
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Registered: 02/04/08
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premix culture powder--need help!
    #7978535 - 02/04/08 04:54 PM (16 years, 15 days ago)

i bought a big bottle of premix dextrose/potato/agar mix off ebay. i followed the directions on the bottle and made the agar, which was 39g in one liter of water, boil to dissolve and autoclave at 15psi for 20 min. when they came out of the pressure cooker the agar solution was hard and jelly-like; i can't mix it up no matter how much i shake. so here is my question: how many grams should i use per liter of water?


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OfflineRogerRabbitM
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Re: premix culture powder--need help! [Re: usagolden]
    #7978613 - 02/04/08 05:04 PM (16 years, 15 days ago)

20 grams of agar agar per liter. You're supposed to pour your petri dishes before the agar cools enough to solidify. You can often re-heat it to get it liquid again.
RR


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OfflineMYSTIQUE
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Re: premix culture powder--need help! [Re: usagolden]
    #7978618 - 02/04/08 05:05 PM (16 years, 15 days ago)

I'm not pro with agar but is it still hot? You have to double boil the agar I believe to get it to go liquid then you can pour it.


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Offlineusagolden
A Tin Can Banana
Registered: 02/04/08
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Re: premix culture powder--need help! [Re: RogerRabbit]
    #7978664 - 02/04/08 05:12 PM (16 years, 15 days ago)

i was planning on doing some jars, not dishes. is this still possible with the potato/dextrose/agar?


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OfflineMYSTIQUE
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Re: premix culture powder--need help! [Re: usagolden]
    #7978675 - 02/04/08 05:14 PM (16 years, 15 days ago)

Are you trying to make agar or a l.c.? Tell us what you are planing. If you warm it up ya You can pour it into a jar and let it harden again.


--------------------
Dont know what the fuck I just said? READ THIS
http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary
I ain't a hippy but I'm covered in dirt                         
Sippin lots of mushroom tea in a tye-dye shirt
Chasin' the Grateful Dead, no shoes on my feet
Beggin' in the parking lot for something to eat,

:onfire:DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!:onfire:


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Left Coast Kratom Shop: Kratom Powder For Sale


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