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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Your Sterile Procedure
#7977622 - 02/04/08 01:10 PM (15 years, 11 months ago) |
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First I PC everything I'm going to use besides the, ya know manure, casings. The PCed stuff is sitting since overnight waiting to be used
I first put some socks on and wipe the bathroom clean with ammonia green scent so its not so strong, I've been knocked out by the stuff twice already so a mask is recommended by me. I then wait about an hour for it to dry. Come back and spray the hell out of the walls with 10% bleach, 90% water to kill any bacteria, contaminants that may have landed on it. Wipe it down and spray every surface including the toilet with Lysol disinfect 99%, I will be soon moving to CLOROX AEROSOL disinfect for this has quat ammonious in it....recommended by Dr.s and janitors and one of the few that can kill AIDS. I then spray the entire bathroom again with bleach solution in the air and surface and wipe down one last time with a new clean rag. Including the doorknob in and out as well as the door. I then leave the room onto my next mission.
I go to the other bathroom and shave my head and body arm hair, facial hair. Brush, Floss, Mouthwash before I enter the shower and after words...I take a shower. I like to wash my head with no hair and wash the nubs that stick out with Shampoo Selsun Blue so my hair will keep growing after words with soft dandruff free hair. I then shampoo with Ausee because I love the smell of it ...I wash my body with a Irish Spring because it makes me feel fresh and clean...Ok. Now, I get out of the shower and use a non alcoholic face creame for the reason because with alcohol dries out your face creating wrinkles in the long run making you look older faster. I then dry myself off and use deodorant and once again Brush, Floss, 99.9% Germ Killer Listerine Mouthwash, the green kind. I then spray myself down naked head included with 10% bleach, 90% water solution which is probably not healthy but I've never gotten sick so....Better Safe Then Sorry. I put my clothes on and spray myself down again with 10% bleach, 90% water and spray my head again(Before all this I got all the dirt and mud out of my shoes and made em look like new again, polishing was included.
I then wipe my hands with 99.9% purell hand sanitizer as it works better then washing your hands because actually when you wash your hands your just taking off a very small layer of skin which takes with it the bacteria and contaminants, when that's drying the air is putting those contaminants right back onto the hands again so I find no use in washing your hands. I go into the other bathroom and spray OUSTS aerosol which is quite generic and cheap if you ask me. I will be moving onto OZIUM...Ozium is recommended by Dr's for hospital sanitation, used greatly throughout laboratories, surgeries. Finer fog works better also. Although it is quite expensive a case of 12 with costs about $100 will last you a good 2 years. A spray which last about 5-6 seconds will kill 90% of the bacteria in the air. After I spray I leave the room for 10 minutes for it the air to settle. After the time has suspended I go in and put my gloves on, Dr's mask used in surgeries, Water resistant Shoe Covers and start my procedure
I first douse my gloves with 70% ISO(I like it better and most mycologists do also because it evaporates slower then 91%+. I then bring out my square beauty cotton swabs and douse them in alcohol and wipe down the syringe and jar. Flame sterilize the needle, wipe down an additional time and inoculate.
Edited by dumbfounded1600 (02/04/08 01:11 PM)
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shevanel
Gone til November



Registered: 01/27/08
Posts: 1,517
Last seen: 1 year, 11 months
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r u serious
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Day Tripper
Got a goodreason, for taking the easyway out.


Registered: 01/27/08
Posts: 198
Loc: Rocky Mountains
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Wow, now that's comprehensive, I'll bet you are at maybe one contamed jar per year huh?
That was awesome, 5 shrooms for you man!
-------------------- Death is inevitable, and therefore irrelevant, life is optional, and therefore irreplaceable.
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BEEP

Registered: 01/06/08
Posts: 1,385
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a glove box would be more efficiant IMO then soaking the air in bleech.
i also cover my arms in saran wrap and soak them in 91% alchohol
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Day Tripper
Got a goodreason, for taking the easyway out.


Registered: 01/27/08
Posts: 198
Loc: Rocky Mountains
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Re: Your Sterile Procedure [Re: BEEP]
#7977671 - 02/04/08 01:25 PM (15 years, 11 months ago) |
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Good call on the saran wrap, better than garbage bags...LOL...
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shevanel
Gone til November



Registered: 01/27/08
Posts: 1,517
Last seen: 1 year, 11 months
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Re: Your Sterile Procedure [Re: BEEP]
#7977674 - 02/04/08 01:26 PM (15 years, 11 months ago) |
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i put my glovebox on a tv tray in front of the comode so I can poop while innoculating my jars...
No seriously, some of the thingsd in that method seem standard, but shaving your head? Spraying yourself with bleach water repeatedly?
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: Your Sterile Procedure [Re: shevanel]
#7977684 - 02/04/08 01:30 PM (15 years, 11 months ago) |
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O and Glad Cling Wrap serves as a generic Parafilm
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: Your Sterile Procedure [Re: shevanel]
#7977690 - 02/04/08 01:32 PM (15 years, 11 months ago) |
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Quote:
shevanel said: i put my glovebox on a tv tray in front of the comode so I can poop while innoculating my jars...
No seriously, some of the thingsd in that method seem standard, but shaving your head? Spraying yourself with bleach water repeatedly?
I find no other method...that works...soon i'll be spraying myself with Clorox disinfectant
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shevanel
Gone til November



Registered: 01/27/08
Posts: 1,517
Last seen: 1 year, 11 months
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Ok, I believe you.
At first I thought you were being funny..
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: Your Sterile Procedure [Re: shevanel]
#7977698 - 02/04/08 01:35 PM (15 years, 11 months ago) |
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No lie there...It's actually a Routine now
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mycosis



Registered: 08/20/07
Posts: 19,727
Loc: USSA
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Re: Your Sterile Procedure [Re: shevanel]
#7977702 - 02/04/08 01:36 PM (15 years, 11 months ago) |
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i needed a laugh like that...thanks!
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Neobean
Adept Mycologist


Registered: 10/07/01
Posts: 975
Loc: Canada
Last seen: 3 years, 8 months
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Re: Your Sterile Procedure [Re: shevanel]
#7977707 - 02/04/08 01:37 PM (15 years, 11 months ago) |
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Quote:
shevanel said: i put my glovebox on a tv tray in front of the comode so I can poop while innoculating my jars...
No seriously, some of the thingsd in that method seem standard, but shaving your head? Spraying yourself with bleach water repeatedly?
The dood is dedicated...5 shrooms!
   
-------------------- If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!
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shevanel
Gone til November



Registered: 01/27/08
Posts: 1,517
Last seen: 1 year, 11 months
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Re: Your Sterile Procedure [Re: Neobean]
#7977716 - 02/04/08 01:39 PM (15 years, 11 months ago) |
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You make me feel inadequate and you give me day dreams of my freshly juiiced jars just sitting upstairs manufacturing mold of all types
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Neobean
Adept Mycologist


Registered: 10/07/01
Posts: 975
Loc: Canada
Last seen: 3 years, 8 months
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Re: Your Sterile Procedure [Re: shevanel]
#7977749 - 02/04/08 01:50 PM (15 years, 11 months ago) |
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You can create the same environment in your glove box, don't be too worried This hobby, believe it or not, is not an all or nothing hobby. There is a middle ground, and a glove box is just that.
-------------------- If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!
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Day Tripper
Got a goodreason, for taking the easyway out.


Registered: 01/27/08
Posts: 198
Loc: Rocky Mountains
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Re: Your Sterile Procedure [Re: Neobean]
#7977753 - 02/04/08 01:51 PM (15 years, 11 months ago) |
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He speaks the truth about the GB, they rock, so hard!
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: Your Sterile Procedure [Re: Day Tripper]
#7977800 - 02/04/08 02:04 PM (15 years, 11 months ago) |
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Yes they do...I'd go with a laminar flow hood any day though
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iTrip
Electronic Trip



Registered: 11/21/07
Posts: 68
Last seen: 14 years, 6 months
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Re: Your Sterile Procedure [Re: shevanel]
#7977883 - 02/04/08 02:29 PM (15 years, 11 months ago) |
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DAMN! thats way too much work for my lazy ass...
i actually use my oven.. turn it to the lowest setting possible
when it starts to get hot enough i place my jars on the bottom shelf and procced with normal INNOC>. and since ive been using this method i havent gotten 1 single contam jar!
Also a lit candle can provide 1-2 sq feet of sterile air space
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: Your Sterile Procedure [Re: iTrip]
#7977901 - 02/04/08 02:33 PM (15 years, 11 months ago) |
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Quote:
iTrip said: DAMN! thats way too much work for my lazy ass...
i actually use my oven.. turn it to the lowest setting possible
when it starts to get hot enough i place my jars on the bottom shelf and procced with normal INNOC>. and since ive been using this method i havent gotten 1 single contam jar!
Also a lit candle can provide 1-2 sq feet of sterile air space
Ovens suck unless you can crawl in there yourself to inoculate...and a lit candle? *COUGH* sorry....I was coughing on the bullshit...they'll actually try to surface more because there attracted to heat.
Edited by dumbfounded1600 (02/04/08 02:34 PM)
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iTrip
Electronic Trip



Registered: 11/21/07
Posts: 68
Last seen: 14 years, 6 months
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Quote:
dumbfounded1600 said:
Quote:
iTrip said: DAMN! thats way too much work for my lazy ass...
i actually use my oven.. turn it to the lowest setting possible
when it starts to get hot enough i place my jars on the bottom shelf and procced with normal INNOC>. and since ive been using this method i havent gotten 1 single contam jar!
Also a lit candle can provide 1-2 sq feet of sterile air space
Ovens suck unless you can crawl in there yourself to inoculate...and a lit candle? *COUGH* sorry....I was coughing on the bullshit...they'll actually try to surface more because there attracted to heat.
well its worked for me.! ive had no contamd jars since i started using this method.!
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P.Menace
Contradicting the Status Quo




Registered: 11/17/07
Posts: 4,797
Loc: Let Me See Potato Salad!!...
Last seen: 9 years, 10 months
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Re: Your Sterile Procedure [Re: Day Tripper]
#7977937 - 02/04/08 02:46 PM (15 years, 11 months ago) |
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Get a flow hood or glove box! I just use a cardboard box with a window cut in the top and covered with a picture frame glass. and a hole big enough to slip in a dozen Qt jars in the box the jars came in. I throw in a light when Im going to use it, Nuke the entire thing with Lysol, Oust, Clorox, whatever I have, I use hand sanitizer always, latex gloves when I have them. 100% success. well unless you count the 1 jar that may have been missed or there was no mycelium in the syringe when inoculated, But for 181 jars Thats great. Plus it proves my practices by showing capability to produce many many many clean viable LC's from clone , SS, SP and previous LC's.
--------------------
http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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shroober
Myco Junkie

Registered: 01/02/08
Posts: 879
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Re: Your Sterile Procedure [Re: iTrip]
#7977973 - 02/04/08 02:58 PM (15 years, 11 months ago) |
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you guys are crazy...
lots of lysol, lots of rubbing alcohol, and one glove box, rarely get contams
i cant believe you shave your body and then spray yourself down with bleach. seems like it would be easier to just get contams every once in a while compared to doing all that
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: Your Sterile Procedure [Re: iTrip]
#7978151 - 02/04/08 03:40 PM (15 years, 11 months ago) |
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Quote:
iTrip said: DAMN! thats way too much work for my lazy ass...
i actually use my oven.. turn it to the lowest setting possible
when it starts to get hot enough i place my jars on the bottom shelf and procced with normal INNOC>. and since ive been using this method i havent gotten 1 single contam jar!
Also a lit candle can provide 1-2 sq feet of sterile air space
YOU HEAR THAT? Its the silent sound of...bullshit
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thedefone
deus ex machina

Registered: 10/06/07
Posts: 1,883
Loc: Gondwana
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Quote:
After I spray I leave the room for 10 minutes for it the air to settle. After the time has suspended I go in and put my gloves on... and start my procedure
And, Tada!! Contaminated work space. Surgeons don't do that much before open-heart surgery.
Quote:
I first douse my gloves with 70% ISO(I like it better and most mycologists do also because it evaporates slower then 91%+. I then bring out my square beauty cotton swabs and douse them in alcohol and wipe down the syringe and jar. Flame sterilize the needle, wipe down an additional time and inoculate.
Add a glovebox, and that's all you really need. Done.
--------------------
I am become death, the destroyer of worlds.
Edited by thedefone (02/04/08 05:22 PM)
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iTrip
Electronic Trip



Registered: 11/21/07
Posts: 68
Last seen: 14 years, 6 months
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Quote:
dumbfounded1600 said:
Quote:
iTrip said: DAMN! thats way too much work for my lazy ass...
i actually use my oven.. turn it to the lowest setting possible
when it starts to get hot enough i place my jars on the bottom shelf and procced with normal INNOC>. and since ive been using this method i havent gotten 1 single contam jar!
Also a lit candle can provide 1-2 sq feet of sterile air space
YOU HEAR THAT? Its the silent sound of...bullshit
have you ever done it?? because like i said...this WORKED for me so i dont care what u say
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: Your Sterile Procedure [Re: iTrip]
#7978461 - 02/04/08 04:41 PM (15 years, 11 months ago) |
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Anyone Else Would Like To Share There Sterile Procedure?
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galadar


Registered: 05/09/07
Posts: 981
Loc: in a monotub!
Last seen: 2 years, 2 months
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why dont i just hook up my showerhead to a tank of alcohol and work in a clean shower of alcohol.
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doitagain
He-Bro



Registered: 02/22/07
Posts: 1,947
Loc: Land of Milk and Honey
Last seen: 13 years, 7 months
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Re: Your Sterile Procedure [Re: iTrip]
#7981428 - 02/05/08 03:27 AM (15 years, 11 months ago) |
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really, your oven? so like how hot do you make it?
-------------------- now i hear the police comin after me
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Serial
Cake or Death?




Registered: 11/10/07
Posts: 58
Loc: Middle of Nowhere
Last seen: 8 years, 11 months
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Re: Your Sterile Procedure [Re: P.Menace]
#7982113 - 02/05/08 10:41 AM (15 years, 11 months ago) |
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I thought I was overkill... I shower 2hrs before innoc to get the bathroom good and steamy, 10% bleach the air, and stick the hepa in there. 1 hour before I hit the air again and then move my gear in. Innoc time I glove/mask up light up the alco lamp, get rid of the hepa, lysol down the surfaces and wash off with isopropyl.. I flame the needle after every 2 innoc's
so far no contaminated jars *knock on wood*
--------------------
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sandman420
Saint PP


Registered: 06/17/04
Posts: 5,384
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Re: Your Sterile Procedure [Re: Serial]
#7982271 - 02/05/08 11:22 AM (15 years, 11 months ago) |
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1) Remove your carpet and replace with vinyl flooring (Cost you about $130 for a 10x10 room)
2) Make a glovebox or a flowhood or both!
3) Put the pipe/bottle down when your doing sterile work
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: Your Sterile Procedure [Re: sandman420]
#7982311 - 02/05/08 11:32 AM (15 years, 11 months ago) |
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Also a lit candle can provide 1-2 sq feet of sterile air space
HAHAHA...Everyone let's point and laugh.
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shroober
Myco Junkie

Registered: 01/02/08
Posts: 879
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i still say the best method is to use sandpaper to scratch all of your skin off, then scrub your glowing red body with bleach and alcohol, then flame sterilize the tips of your fingers and while still naked crawl into the oven and proceed with inoculation, this method has always worked well for me, individual results may vary
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shevanel
Gone til November



Registered: 01/27/08
Posts: 1,517
Last seen: 1 year, 11 months
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Re: Your Sterile Procedure [Re: shroober]
#7982580 - 02/05/08 12:54 PM (15 years, 11 months ago) |
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Quote:
shroober said: i still say the best method is to use sandpaper to scratch all of your skin off, then scrub your glowing red body with bleach and alcohol, then flame sterilize the tips of your fingers and while still naked crawl into the oven and proceed with inoculation, this method has always worked well for me, individual results may vary
lol that and teh shower of ackahall is hilarious!
Whatchoo smokin?
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P.Menace
Contradicting the Status Quo




Registered: 11/17/07
Posts: 4,797
Loc: Let Me See Potato Salad!!...
Last seen: 9 years, 10 months
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Re: Your Sterile Procedure [Re: shevanel]
#7983224 - 02/05/08 04:14 PM (15 years, 11 months ago) |
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My pipe/bong has no effect on sterile work, In a positive flow Glovebox. Sandpaper/Oven TEK pics? Lol
--------------------
http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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Captain Cubensis
Bleeding HeartLiberal


Registered: 09/18/07
Posts: 648
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Re: Your Sterile Procedure [Re: P.Menace]
#7983237 - 02/05/08 04:16 PM (15 years, 11 months ago) |
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All you need is 91% alcohol, and a glove box, that's it, nothing else is needed to be sterile.
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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91% sucks...70% is better because it takes longer to evaporate...every experienced mycologists would say the same.
O yeah after I go in the bathroom I spray ousts/Ozium again .... Ozium knocks ousts socks off any day though...Oust is just for soccer moms coming home from work not having to smell the bad odor.
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Izord
Stranger


Registered: 02/05/08
Posts: 44
Last seen: 14 years, 4 months
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Re: Your Sterile Procedure [Re: P.Menace]
#7983462 - 02/05/08 05:13 PM (15 years, 11 months ago) |
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I used to do all that, now I just order some spore syringes and shoot the noc through a filter disk into sterile quart jars. Contams have no chance to contact the grain till its fully colonized and the mycelium is strong.
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: Your Sterile Procedure [Re: Izord]
#7983485 - 02/05/08 05:17 PM (15 years, 11 months ago) |
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Quote:
Izord said: I used to do all that, now I just order some spore syringes and shoot the noc through a filter disk into sterile quart jars. Contams have no chance to contact the grain till its fully colonized and the mycelium is strong.
Nice man
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Serial
Cake or Death?




Registered: 11/10/07
Posts: 58
Loc: Middle of Nowhere
Last seen: 8 years, 11 months
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I can top all that, I go through full Level 5 decontamination. See the andromeda strain. I tell ya that xenon flash gets real old when your just knocking up a couple of jars.
--------------------
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: Your Sterile Procedure [Re: P.Menace]
#8040803 - 02/18/08 11:07 PM (15 years, 11 months ago) |
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Would anyone else like to share?
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xile


Registered: 10/04/07
Posts: 63
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Oust the air, PC jars w/ grain. Oust the air, soak needle in alcohol, burn needle, cool in alcohol for a second, inject. Wipe injection point with alcohol. Oh, and wash hands for both steps. Bout the only sterile things I do and it works.
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: Your Sterile Procedure [Re: xile]
#8040881 - 02/18/08 11:22 PM (15 years, 11 months ago) |
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IMHO, There's no need to soak the needle with alcohol after it's already bean burnt. You are giving it time to access contaminants.
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Jadis
..Just waituntil you seethe bats!

Registered: 08/19/04
Posts: 227
Last seen: 13 years, 8 months
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I work with bacteria on a pretty daily basis and a hot flame WILL provide some cubic ft of air space. Learned that in like freshman biology lab.
A lit candle? It will provide some, but probably not that much. I'm thinking more along the lines of a bunsen burner.
Quote:
dumbfounded1600 said: Also a lit candle can provide 1-2 sq feet of sterile air space
HAHAHA...Everyone let's point and laugh.
-------------------- Do you realize that everyone you know someday will die? And instead of saying all of your goodbyes, Let them know you realize that life goes fast. It's hard to make the good things last. You realize the sun don't go down; It's just an illusion caused by the world spinnin' round.
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xile


Registered: 10/04/07
Posts: 63
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I thought the purpose of alcohol was to you know.. kill contams? Plus, wouldn't the needle be too hot that it'd burn the spores coming through it?
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: Your Sterile Procedure [Re: Jadis]
#8040910 - 02/18/08 11:26 PM (15 years, 11 months ago) |
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Not even close...I challenge anyone to this.
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Jadis
..Just waituntil you seethe bats!

Registered: 08/19/04
Posts: 227
Last seen: 13 years, 8 months
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If you're using a spray bottle, like you should be, then what are you worried about evaporation for? In fact, I'd probably want it to evaporate faster just to hurry up. It's my belief that 70% is the norm since anything above 70% is really just a waste of ethanol (since 70% will have the same toxic effects as 100%).
Quote:
dumbfounded1600 said: 91% sucks...70% is better because it takes longer to evaporate...every experienced mycologists would say the same.
O yeah after I go in the bathroom I spray ousts/Ozium again .... Ozium knocks ousts socks off any day though...Oust is just for soccer moms coming home from work not having to smell the bad odor.
-------------------- Do you realize that everyone you know someday will die? And instead of saying all of your goodbyes, Let them know you realize that life goes fast. It's hard to make the good things last. You realize the sun don't go down; It's just an illusion caused by the world spinnin' round.
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: Your Sterile Procedure [Re: Jadis]
#8040952 - 02/18/08 11:34 PM (15 years, 11 months ago) |
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Alcohol is not an air sanitizer, it's a surface disinfectant. Big Difference! Using alcohol as an air sanitizer will just make your room a waiting fireball.
Sanitize means to reduce the number of contaminants to a safe or relatively safe level as may be judged by public health requirements. Disinfect means elimination of all recognized pathogenic microorganism but not necessarily all microbial forms. Sterilize means the destruction of all microbical life by use of chemical or physical procedures.
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Jadis
..Just waituntil you seethe bats!

Registered: 08/19/04
Posts: 227
Last seen: 13 years, 8 months
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Was your post directed at me? Because I wasn't trying to say alcohol is an air sanitizer. I just meant when I use alcohol I like to use a spray bottle to apply it to surfaces.
-------------------- Do you realize that everyone you know someday will die? And instead of saying all of your goodbyes, Let them know you realize that life goes fast. It's hard to make the good things last. You realize the sun don't go down; It's just an illusion caused by the world spinnin' round.
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: Your Sterile Procedure [Re: Jadis]
#8040986 - 02/18/08 11:40 PM (15 years, 11 months ago) |
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O...usually people just wash there hands with it, It wasn't directly at you no..I thought you were talking about something else.
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Jadis
..Just waituntil you seethe bats!

Registered: 08/19/04
Posts: 227
Last seen: 13 years, 8 months
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yeah i thought we mightve had a mixup! haha. umm i like this thread though it helps me prepare to try my first time!
-------------------- Do you realize that everyone you know someday will die? And instead of saying all of your goodbyes, Let them know you realize that life goes fast. It's hard to make the good things last. You realize the sun don't go down; It's just an illusion caused by the world spinnin' round.
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: Your Sterile Procedure [Re: Jadis]
#8041007 - 02/18/08 11:46 PM (15 years, 11 months ago) |
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Read my sterile technique on the first page...OCD..
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Jadis
..Just waituntil you seethe bats!

Registered: 08/19/04
Posts: 227
Last seen: 13 years, 8 months
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yeah im not gonna do all that lol
-------------------- Do you realize that everyone you know someday will die? And instead of saying all of your goodbyes, Let them know you realize that life goes fast. It's hard to make the good things last. You realize the sun don't go down; It's just an illusion caused by the world spinnin' round.
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playwithguns
Sporophore

Registered: 10/20/04
Posts: 223
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Re: Your Sterile Procedure [Re: Jadis]
#8041536 - 02/19/08 03:40 AM (15 years, 11 months ago) |
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What sterile procedures? Just keep yer grubby fingers outta there.
-------------------- My Garden
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Peterthinks
(Caulking) gun for hire


Registered: 11/10/04
Posts: 2,379
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Re: Your Sterile Procedure [Re: P.Menace]
#8042009 - 02/19/08 09:23 AM (15 years, 11 months ago) |
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sometimes I just hold my breath. If I just wanna grab a quick clone and spike it into a dish or jar I seriously just crank on the oven element, stick the blade on it and when it's red I just do it as smooth and fast as I can. Split the shroom, cut the sample drop it in, shut it in....Breathe... Right in the middle of my filthy kitchen.
you'd be suprised how often I've gotten away with it.
If I'm doing a lot of stuff I shut off the furnace around midnight and do it REAL early before anyone has had a chance to kick up any dust. One time my wife turned on the vacuum while I was working....totally fucked everything I had open and every dish I tried to do after that. I am working on another glove box even though I haven't had many problems (except the vacuum thing)
-------------------- Give a man a fire and he will be warm for the rest of the night. Set a man on fire and he will be warm for the rest of his life. NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS? Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: Your Sterile Procedure [Re: Peterthinks]
#8042044 - 02/19/08 09:37 AM (15 years, 11 months ago) |
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Even when I'm in the most sterile room which lab's don't even have but I don't even breathe...If I do..I'll either breathe out the window or into the fan of the laminar flow hood.
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Peterthinks
(Caulking) gun for hire


Registered: 11/10/04
Posts: 2,379
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You have the window open in your "most sterile room"?
-------------------- Give a man a fire and he will be warm for the rest of the night. Set a man on fire and he will be warm for the rest of his life. NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS? Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: Your Sterile Procedure [Re: Peterthinks]
#8042070 - 02/19/08 09:49 AM (15 years, 11 months ago) |
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Glovebox with a window open so I can blow out. I sterilize it from cm to cm then I hold my breathe even after I brush/Mouthwash, Shave Head, Face, Shower, all the above in my sterile technique just 'better safe than sorry.
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meatman
Goner



Registered: 04/22/01
Posts: 439
Loc: Underwater Lake
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Know what I do? I pressure cook jars and whatever i put in them. i flame sterilize my needle, then wipe it with alcohol. inoculate. done. no bleach, no oust, no bullshit. Contamination has never been a problem for me. I don't even have a glovebox. I could give a rat's ass if I lose 1 or 2 jars to contam. Or even a bag, since wbs is cheap and having lc means I can do lots of innoculations. I simply cannot believe the things some people do.
-------------------- "I don't do drugs. I am drugs." -Dali Everything I post is a complete fabrication made for your entertainment.
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Jadis
..Just waituntil you seethe bats!

Registered: 08/19/04
Posts: 227
Last seen: 13 years, 8 months
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good luck doing tissue cultures in a sterile room without breathing i practically lived in a sterile room + working in a positive pressure hood and would spend hours in there over the summer. youre definitely overkill dumbfounded, but hey. if it works and you dont mind the extra caution then more props for ya
-------------------- Do you realize that everyone you know someday will die? And instead of saying all of your goodbyes, Let them know you realize that life goes fast. It's hard to make the good things last. You realize the sun don't go down; It's just an illusion caused by the world spinnin' round.
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: Your Sterile Procedure [Re: meatman]
#8042130 - 02/19/08 10:05 AM (15 years, 11 months ago) |
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Quote:
meatman said: Know what I do? I pressure cook jars and whatever i put in them. i flame sterilize my needle, then wipe it with alcohol. inoculate. done. no bleach, no oust, no bullshit. Contamination has never been a problem for me. I don't even have a glovebox. I could give a rat's ass if I lose 1 or 2 jars to contam. Or even a bag, since wbs is cheap and having lc means I can do lots of innoculations. I simply cannot believe the things some people do.
You have a low % of bacterial spores in your house
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: Your Sterile Procedure [Re: Jadis]
#8042140 - 02/19/08 10:08 AM (15 years, 11 months ago) |
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Quote:
Jadis said: good luck doing tissue cultures in a sterile room without breathing i practically lived in a sterile room + working in a positive pressure hood and would spend hours in there over the summer. youre definitely overkill dumbfounded, but hey. if it works and you dont mind the extra caution then more props for ya
I recommend Ozium ... they pick that as the best Air Sanitizer out there....they say Ousts kills 99.9% .. BULL SHIT...Ousts is for Soccor Moms who wanna come home to a non smelly home.
Ozium is recommended for Hospital Sanitiation. Lab Work, Surgeries and has a finer mist! You use about 4-5 sprays and be as effective as using a half to a whole can of OUST! A 14.5 oz can will go for months! Trust me...try it out...I've never had a complaint with it and they recommend it now way better then OUST.
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Please read through 1-3 to understand the meaning of this thread...
Who would like to share there sterile procedure?
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dumbfounded1600
Stranger
Registered: 07/29/07
Posts: 2,624
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Re: Your Sterile Procedure [Re: P.Menace]
#8173113 - 03/20/08 06:52 PM (15 years, 10 months ago) |
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Anyone else like to share..?
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cyberthreat
Stranger


Registered: 03/06/05
Posts: 676
Last seen: 7 years, 7 months
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Re: Your Sterile Procedure [Re: P.Menace]
#8173149 - 03/20/08 07:00 PM (15 years, 10 months ago) |
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full tyvek hooded suit gloves just cant get enough bought three box the other day a mask tyvek booties 3 box of alcohol preps alcohol lamp 2 cans of Lysol and a punch of bottles of alcohol I coat my gloves in alcohol
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Dude, WTF are you inoculating? An open salad bowl full of grains? Any kind of jar or bag with a decent injection site and filter will negate the need for all that B.S.
Man, I hope that was all a joke.
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Quick WBS Prep
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dumbfounded1600
Stranger
Registered: 07/29/07
Posts: 2,624
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Re: Your Sterile Procedure [Re: FooMan]
#8173206 - 03/20/08 07:14 PM (15 years, 10 months ago) |
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Trust me dude, I would not write all that if it was a joke. It'd be a simple sentence. IIII CAN'T LIIIE!!
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large_dose
Melonhead



Registered: 05/05/06
Posts: 1,346
Loc: Right in the Middle
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Re: Your Sterile Procedure [Re: P.Menace]
#8176817 - 03/21/08 05:47 PM (15 years, 10 months ago) |
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dude like, i stopped after the first sentance second paragraph. 
Quote:
I first put some socks on and wipe the bathroom clean with ammonia green scent so its not so strong, I've been knocked out by the stuff twice already so a mask is recommended by me.
all this isnt necessary man... close the room off from air currents your working in, get yourself a bottle of oust, butane lighter and some alcohol wipes.
glovebox or trashbag tek works great too.
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ReverseOsmosis
Stranger


Registered: 11/11/08
Posts: 370
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Re: Your Sterile Procedure [Re: large_dose]
#9334946 - 11/29/08 11:46 PM (15 years, 2 months ago) |
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WoW! lol!! Talk about overkill.
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P.Menace
Contradicting the Status Quo




Registered: 11/17/07
Posts: 4,797
Loc: Let Me See Potato Salad!!...
Last seen: 9 years, 10 months
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Wow! talk about  bumping an already pointless 8 month old thread with a pointless response
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http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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seven
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Registered: 06/09/08
Posts: 1,478
Loc: north carolina
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Re: Your Sterile Procedure [Re: P.Menace]
#9335809 - 11/30/08 04:12 AM (15 years, 2 months ago) |
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to anyone who reads this whole thread: the op goes stupidly overboard! i am almost positive he has lung damage from breathing those fumes! the adivice about using the oven is crap. all the advice about using a glovebox is good advice. i was amazed at the overkill in this thread! the fact is: its not needed, a waste, and a hazard to your health!
-------------------- grind
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backintheriver



Registered: 08/21/08
Posts: 634
Last seen: 1 year, 6 months
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Re: Your Sterile Procedure [Re: P.Menace]
#9336229 - 11/30/08 08:49 AM (15 years, 2 months ago) |
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do you have any experience with OCD? EDIT: sorry i didnt realize how old this thread is. at least i wasn't the first to bump it. =]
Edited by backintheriver (11/30/08 09:09 AM)
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