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Invisibleshroober
Myco Junkie

Registered: 01/02/08
Posts: 879
Re: Your Sterile Procedure [Re: iTrip]
    #7977973 - 02/04/08 02:58 PM (15 years, 11 months ago)

you guys are crazy...

lots of lysol, lots of rubbing alcohol, and one glove box, rarely get contams

i cant believe you shave your body and then spray yourself down with bleach. seems like it would be easier to just get contams every once in a while compared to doing all that :smile:


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Invisibledumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
Re: Your Sterile Procedure [Re: iTrip]
    #7978151 - 02/04/08 03:40 PM (15 years, 11 months ago)

Quote:

iTrip said:
DAMN! thats way too much work for my lazy ass... :cool:

i actually use my oven.. turn it to the lowest setting possible

when it starts to get hot enough i place my jars on the bottom  shelf and procced with normal INNOC>. and since ive been using this method i havent gotten 1 single contam jar!

Also a lit candle can provide 1-2 sq feet of sterile air space :eek:





YOU HEAR THAT?
Its the silent sound of...bullshit


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Invisiblethedefone
deus ex machina

Registered: 10/06/07
Posts: 1,883
Loc: Gondwana
Re: Your Sterile Procedure [Re: dumbfounded1600]
    #7978200 - 02/04/08 03:54 PM (15 years, 11 months ago)

Quote:

After I spray I leave the room for 10 minutes for it the air to settle. After the time has suspended I go in and put my gloves on... and start my procedure



And, Tada!! Contaminated work space. Surgeons don't do that much before open-heart surgery.

Quote:

I first douse my gloves with 70% ISO(I like it better and most mycologists do also because it evaporates slower then 91%+. I then bring out my square beauty cotton swabs and douse them in alcohol and wipe down the syringe and jar. Flame sterilize the needle, wipe down an additional time and inoculate.



Add a glovebox, and that's all you really need. Done.


--------------------


I am become death, the destroyer of worlds.


Edited by thedefone (02/04/08 05:22 PM)


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OfflineiTrip
Electronic Trip
Male


Registered: 11/21/07
Posts: 68
Last seen: 14 years, 6 months
Re: Your Sterile Procedure [Re: dumbfounded1600]
    #7978385 - 02/04/08 04:30 PM (15 years, 11 months ago)

Quote:

dumbfounded1600 said:
Quote:

iTrip said:
DAMN! thats way too much work for my lazy ass... :cool:

i actually use my oven.. turn it to the lowest setting possible

when it starts to get hot enough i place my jars on the bottom  shelf and procced with normal INNOC>. and since ive been using this method i havent gotten 1 single contam jar!

Also a lit candle can provide 1-2 sq feet of sterile air space :eek:





YOU HEAR THAT?
Its the silent sound of...bullshit




have you ever done it??
because like i said...this WORKED for me so i dont care what u say


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Invisibledumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
Re: Your Sterile Procedure [Re: iTrip]
    #7978461 - 02/04/08 04:41 PM (15 years, 11 months ago)

Anyone Else Would Like To Share There Sterile Procedure?


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Offlinegaladar


Registered: 05/09/07
Posts: 981
Loc: in a monotub!
Last seen: 2 years, 2 months
Re: Your Sterile Procedure [Re: dumbfounded1600]
    #7981237 - 02/05/08 01:05 AM (15 years, 11 months ago)

why dont i just hook up my showerhead to a tank of alcohol and work in a clean shower of alcohol.


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Offlinedoitagain
He-Bro
Male User Gallery


Registered: 02/22/07
Posts: 1,947
Loc: Land of Milk and Honey
Last seen: 13 years, 7 months
Re: Your Sterile Procedure [Re: iTrip]
    #7981428 - 02/05/08 03:27 AM (15 years, 11 months ago)

really, your oven? so like how hot do you make it?


--------------------
now i hear the police comin after me


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OfflineSerial
Cake or Death?
Male


Folding@home Statistics
Registered: 11/10/07
Posts: 58
Loc: Middle of Nowhere
Last seen: 8 years, 11 months
Re: Your Sterile Procedure [Re: P.Menace]
    #7982113 - 02/05/08 10:41 AM (15 years, 11 months ago)

I thought I was overkill...
I shower 2hrs before innoc to get the bathroom good and steamy, 10% bleach the air, and stick the hepa in there. 1 hour before I hit the air again and then move my gear in. Innoc time I glove/mask up light up the alco lamp, get rid of the hepa, lysol down the surfaces and wash off with isopropyl.. I flame the needle after every 2 innoc's

so far no contaminated jars *knock on wood*


--------------------
 


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Invisiblesandman420
Saint PP
Male

Registered: 06/17/04
Posts: 5,384
Trusted Cultivator
Re: Your Sterile Procedure [Re: Serial]
    #7982271 - 02/05/08 11:22 AM (15 years, 11 months ago)

1) Remove your carpet and replace with vinyl flooring (Cost you about $130 for a 10x10 room)

2) Make a glovebox or a flowhood or both!

3) Put the pipe/bottle down when your doing sterile work


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


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Invisibledumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
Re: Your Sterile Procedure [Re: sandman420]
    #7982311 - 02/05/08 11:32 AM (15 years, 11 months ago)

Also a lit candle can provide 1-2 sq feet of sterile air space :eek:






HAHAHA...Everyone let's point and laugh.


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Invisibleshroober
Myco Junkie

Registered: 01/02/08
Posts: 879
Re: Your Sterile Procedure [Re: dumbfounded1600]
    #7982454 - 02/05/08 12:15 PM (15 years, 11 months ago)

i still say the best method is to use sandpaper to scratch all of your skin off, then scrub your glowing red body with bleach and alcohol, then flame sterilize the tips of your fingers and while still naked crawl into the oven and proceed with inoculation, this method has always worked well for me, individual results may vary


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Offlineshevanel
Gone til November
 User Gallery


Registered: 01/27/08
Posts: 1,517
Last seen: 1 year, 11 months
Re: Your Sterile Procedure [Re: shroober]
    #7982580 - 02/05/08 12:54 PM (15 years, 11 months ago)

Quote:

shroober said:
i still say the best method is to use sandpaper to scratch all of your skin off, then scrub your glowing red body with bleach and alcohol, then flame sterilize the tips of your fingers and while still naked crawl into the oven and proceed with inoculation, this method has always worked well for me, individual results may vary




lol that and teh shower of ackahall is hilarious!

Whatchoo smokin?


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OfflineP.Menace
Contradicting the Status Quo
Male User Gallery


Folding@home Statistics
Registered: 11/17/07
Posts: 4,797
Loc: Let Me See Potato Salad!!...
Last seen: 9 years, 10 months
Trusted Cultivator
Re: Your Sterile Procedure [Re: shevanel]
    #7983224 - 02/05/08 04:14 PM (15 years, 11 months ago)

My pipe/bong has no effect on sterile work, In a positive flow Glovebox.
Sandpaper/Oven TEK pics? Lol


--------------------

http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace


roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.


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InvisibleCaptain Cubensis
Bleeding HeartLiberal


Registered: 09/18/07
Posts: 648
Re: Your Sterile Procedure [Re: P.Menace]
    #7983237 - 02/05/08 04:16 PM (15 years, 11 months ago)

All you need is 91% alcohol, and a glove box, that's it, nothing else is needed to be sterile.


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Invisibledumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
Re: Your Sterile Procedure [Re: Captain Cubensis]
    #7983272 - 02/05/08 04:27 PM (15 years, 11 months ago)

91% sucks...70% is better because it takes longer to evaporate...every experienced mycologists would say the same.

O yeah after I go in the bathroom I spray ousts/Ozium again .... Ozium knocks ousts socks off any day though...Oust is just for soccer moms coming home from work not having to smell the bad odor.


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OfflineIzord
Stranger
 User Gallery

Registered: 02/05/08
Posts: 44
Last seen: 14 years, 4 months
Re: Your Sterile Procedure [Re: P.Menace]
    #7983462 - 02/05/08 05:13 PM (15 years, 11 months ago)

I used to do all that, now I just order some spore syringes and shoot the noc through a filter disk into sterile quart jars. Contams have no chance to contact the grain till its fully colonized and the mycelium is strong.


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Invisibledumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
Re: Your Sterile Procedure [Re: Izord]
    #7983485 - 02/05/08 05:17 PM (15 years, 11 months ago)

Quote:

Izord said:
I used to do all that, now I just order some spore syringes and shoot the noc through a filter disk into sterile quart jars. Contams have no chance to contact the grain till its fully colonized and the mycelium is strong.




Nice man


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OfflineSerial
Cake or Death?
Male


Folding@home Statistics
Registered: 11/10/07
Posts: 58
Loc: Middle of Nowhere
Last seen: 8 years, 11 months
Re: Your Sterile Procedure [Re: dumbfounded1600]
    #7983543 - 02/05/08 05:31 PM (15 years, 11 months ago)

I can top all that, I go through full Level 5 decontamination. See the andromeda strain. I tell ya that xenon flash gets real old when your just knocking up a couple of jars.


--------------------
 


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Invisibledumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
Re: Your Sterile Procedure [Re: P.Menace]
    #8040803 - 02/18/08 11:07 PM (15 years, 11 months ago)

Would anyone else like to share?


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Invisiblexile
I'm a teapot User Gallery

Registered: 10/04/07
Posts: 63
Re: Your Sterile Procedure [Re: dumbfounded1600]
    #8040830 - 02/18/08 11:13 PM (15 years, 11 months ago)

Oust the air, PC jars w/ grain. Oust the air, soak needle in alcohol, burn needle, cool in alcohol for a second, inject. Wipe injection point with alcohol. Oh, and wash hands for both steps. Bout the only sterile things I do and it works.


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