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shroober
Myco Junkie

Registered: 01/02/08
Posts: 879
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Re: Your Sterile Procedure [Re: iTrip]
#7977973 - 02/04/08 02:58 PM (15 years, 11 months ago) |
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you guys are crazy...
lots of lysol, lots of rubbing alcohol, and one glove box, rarely get contams
i cant believe you shave your body and then spray yourself down with bleach. seems like it would be easier to just get contams every once in a while compared to doing all that
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: Your Sterile Procedure [Re: iTrip]
#7978151 - 02/04/08 03:40 PM (15 years, 11 months ago) |
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Quote:
iTrip said: DAMN! thats way too much work for my lazy ass...
i actually use my oven.. turn it to the lowest setting possible
when it starts to get hot enough i place my jars on the bottom shelf and procced with normal INNOC>. and since ive been using this method i havent gotten 1 single contam jar!
Also a lit candle can provide 1-2 sq feet of sterile air space
YOU HEAR THAT? Its the silent sound of...bullshit
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thedefone
deus ex machina

Registered: 10/06/07
Posts: 1,883
Loc: Gondwana
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Quote:
After I spray I leave the room for 10 minutes for it the air to settle. After the time has suspended I go in and put my gloves on... and start my procedure
And, Tada!! Contaminated work space. Surgeons don't do that much before open-heart surgery.
Quote:
I first douse my gloves with 70% ISO(I like it better and most mycologists do also because it evaporates slower then 91%+. I then bring out my square beauty cotton swabs and douse them in alcohol and wipe down the syringe and jar. Flame sterilize the needle, wipe down an additional time and inoculate.
Add a glovebox, and that's all you really need. Done.
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I am become death, the destroyer of worlds.
Edited by thedefone (02/04/08 05:22 PM)
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iTrip
Electronic Trip



Registered: 11/21/07
Posts: 68
Last seen: 14 years, 6 months
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Quote:
dumbfounded1600 said:
Quote:
iTrip said: DAMN! thats way too much work for my lazy ass...
i actually use my oven.. turn it to the lowest setting possible
when it starts to get hot enough i place my jars on the bottom shelf and procced with normal INNOC>. and since ive been using this method i havent gotten 1 single contam jar!
Also a lit candle can provide 1-2 sq feet of sterile air space
YOU HEAR THAT? Its the silent sound of...bullshit
have you ever done it?? because like i said...this WORKED for me so i dont care what u say
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: Your Sterile Procedure [Re: iTrip]
#7978461 - 02/04/08 04:41 PM (15 years, 11 months ago) |
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Anyone Else Would Like To Share There Sterile Procedure?
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galadar


Registered: 05/09/07
Posts: 981
Loc: in a monotub!
Last seen: 2 years, 2 months
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why dont i just hook up my showerhead to a tank of alcohol and work in a clean shower of alcohol.
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doitagain
He-Bro



Registered: 02/22/07
Posts: 1,947
Loc: Land of Milk and Honey
Last seen: 13 years, 7 months
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Re: Your Sterile Procedure [Re: iTrip]
#7981428 - 02/05/08 03:27 AM (15 years, 11 months ago) |
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really, your oven? so like how hot do you make it?
-------------------- now i hear the police comin after me
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Serial
Cake or Death?




Registered: 11/10/07
Posts: 58
Loc: Middle of Nowhere
Last seen: 8 years, 11 months
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Re: Your Sterile Procedure [Re: P.Menace]
#7982113 - 02/05/08 10:41 AM (15 years, 11 months ago) |
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I thought I was overkill... I shower 2hrs before innoc to get the bathroom good and steamy, 10% bleach the air, and stick the hepa in there. 1 hour before I hit the air again and then move my gear in. Innoc time I glove/mask up light up the alco lamp, get rid of the hepa, lysol down the surfaces and wash off with isopropyl.. I flame the needle after every 2 innoc's
so far no contaminated jars *knock on wood*
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sandman420
Saint PP


Registered: 06/17/04
Posts: 5,384
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Re: Your Sterile Procedure [Re: Serial]
#7982271 - 02/05/08 11:22 AM (15 years, 11 months ago) |
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1) Remove your carpet and replace with vinyl flooring (Cost you about $130 for a 10x10 room)
2) Make a glovebox or a flowhood or both!
3) Put the pipe/bottle down when your doing sterile work
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: Your Sterile Procedure [Re: sandman420]
#7982311 - 02/05/08 11:32 AM (15 years, 11 months ago) |
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Also a lit candle can provide 1-2 sq feet of sterile air space
HAHAHA...Everyone let's point and laugh.
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shroober
Myco Junkie

Registered: 01/02/08
Posts: 879
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i still say the best method is to use sandpaper to scratch all of your skin off, then scrub your glowing red body with bleach and alcohol, then flame sterilize the tips of your fingers and while still naked crawl into the oven and proceed with inoculation, this method has always worked well for me, individual results may vary
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shevanel
Gone til November



Registered: 01/27/08
Posts: 1,517
Last seen: 1 year, 11 months
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Re: Your Sterile Procedure [Re: shroober]
#7982580 - 02/05/08 12:54 PM (15 years, 11 months ago) |
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Quote:
shroober said: i still say the best method is to use sandpaper to scratch all of your skin off, then scrub your glowing red body with bleach and alcohol, then flame sterilize the tips of your fingers and while still naked crawl into the oven and proceed with inoculation, this method has always worked well for me, individual results may vary
lol that and teh shower of ackahall is hilarious!
Whatchoo smokin?
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P.Menace
Contradicting the Status Quo




Registered: 11/17/07
Posts: 4,797
Loc: Let Me See Potato Salad!!...
Last seen: 9 years, 10 months
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Re: Your Sterile Procedure [Re: shevanel]
#7983224 - 02/05/08 04:14 PM (15 years, 11 months ago) |
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My pipe/bong has no effect on sterile work, In a positive flow Glovebox. Sandpaper/Oven TEK pics? Lol
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http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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Captain Cubensis
Bleeding HeartLiberal


Registered: 09/18/07
Posts: 648
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Re: Your Sterile Procedure [Re: P.Menace]
#7983237 - 02/05/08 04:16 PM (15 years, 11 months ago) |
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All you need is 91% alcohol, and a glove box, that's it, nothing else is needed to be sterile.
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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91% sucks...70% is better because it takes longer to evaporate...every experienced mycologists would say the same.
O yeah after I go in the bathroom I spray ousts/Ozium again .... Ozium knocks ousts socks off any day though...Oust is just for soccer moms coming home from work not having to smell the bad odor.
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Izord
Stranger


Registered: 02/05/08
Posts: 44
Last seen: 14 years, 4 months
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Re: Your Sterile Procedure [Re: P.Menace]
#7983462 - 02/05/08 05:13 PM (15 years, 11 months ago) |
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I used to do all that, now I just order some spore syringes and shoot the noc through a filter disk into sterile quart jars. Contams have no chance to contact the grain till its fully colonized and the mycelium is strong.
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: Your Sterile Procedure [Re: Izord]
#7983485 - 02/05/08 05:17 PM (15 years, 11 months ago) |
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Quote:
Izord said: I used to do all that, now I just order some spore syringes and shoot the noc through a filter disk into sterile quart jars. Contams have no chance to contact the grain till its fully colonized and the mycelium is strong.
Nice man
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Serial
Cake or Death?




Registered: 11/10/07
Posts: 58
Loc: Middle of Nowhere
Last seen: 8 years, 11 months
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I can top all that, I go through full Level 5 decontamination. See the andromeda strain. I tell ya that xenon flash gets real old when your just knocking up a couple of jars.
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: Your Sterile Procedure [Re: P.Menace]
#8040803 - 02/18/08 11:07 PM (15 years, 11 months ago) |
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Would anyone else like to share?
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xile


Registered: 10/04/07
Posts: 63
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Oust the air, PC jars w/ grain. Oust the air, soak needle in alcohol, burn needle, cool in alcohol for a second, inject. Wipe injection point with alcohol. Oh, and wash hands for both steps. Bout the only sterile things I do and it works.
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