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Shop: Mushroom-Hut Liquid Cultures, Substrate Mix   North Spore Bulk Substrate, Injection Grain Bag

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Invisibledumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
Your Sterile Procedure
    #7977622 - 02/04/08 01:10 PM (15 years, 11 months ago)

First I PC everything I'm going to use besides the, ya know manure, casings. The PCed stuff is sitting since overnight waiting to be used


I first put some socks on and wipe the bathroom clean with ammonia green scent so its not so strong, I've been knocked out by the stuff twice already so a mask is recommended by me. I then wait about an hour for it to dry. Come back and spray the hell out of the walls with 10% bleach, 90% water to kill any bacteria, contaminants that may have landed on it. Wipe it down and spray every surface including the toilet with Lysol disinfect 99%, I will be soon moving to CLOROX AEROSOL disinfect for this has quat ammonious in it....recommended by Dr.s and janitors and one of the few that can kill AIDS. I then spray the entire bathroom again with bleach solution in the air and surface and wipe down one last time with a new clean rag. Including the doorknob in and out as well as the door. I then leave the room onto my next mission.


I go to the other bathroom and shave my head and body arm hair, facial hair. Brush, Floss, Mouthwash before I enter the shower and after words...I take a shower. I like to wash my head with no hair and wash the nubs that stick out with Shampoo Selsun Blue so my hair will keep growing after words with soft dandruff free hair. I then shampoo with Ausee because I love the smell of it :smile:...I wash my body with a Irish Spring because it makes me feel fresh and clean...Ok. Now, I get out of the shower and use a non alcoholic face creame for the reason because with alcohol dries out your face creating wrinkles in the long run making you look older faster. I then dry myself off and use deodorant and once again Brush, Floss, 99.9% Germ Killer Listerine Mouthwash, the green kind. I then spray myself down naked head included with 10% bleach, 90% water solution which is probably not healthy but I've never gotten sick so....Better Safe Then Sorry. I put my clothes on and spray myself down again with 10% bleach, 90% water and spray my head again(Before all this I got all the dirt and mud out of my shoes and made em look like new again, polishing was included.


I then wipe my hands with 99.9% purell hand sanitizer as it works better then washing your hands because actually when you wash your hands your just taking off a very small layer of skin which takes with it the bacteria and contaminants, when that's drying the air is putting those contaminants right back onto the hands again so I find no use in washing your hands. I go into the other bathroom and spray OUSTS aerosol which is quite generic and cheap if you ask me. I will be moving onto OZIUM...Ozium is recommended by Dr's for hospital sanitation, used greatly throughout laboratories, surgeries. Finer fog works better also. Although it is quite expensive a case of 12 with costs about $100 will last you a good 2 years. A spray which last about 5-6 seconds will kill 90% of the bacteria in the air. After I spray I leave the room for 10 minutes for it the air to settle. After the time has suspended I go in and put my gloves on, Dr's mask used in surgeries, Water resistant Shoe Covers and start my procedure


I first douse my gloves with 70% ISO(I like it better and most mycologists do also because it evaporates slower then 91%+. I then bring out my square beauty cotton swabs and douse them in alcohol and wipe down the syringe and jar. Flame sterilize the needle, wipe down an additional time and inoculate.


Edited by dumbfounded1600 (02/04/08 01:11 PM)


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Offlineshevanel
Gone til November
 User Gallery


Registered: 01/27/08
Posts: 1,517
Last seen: 1 year, 11 months
Re: Your Sterile Procedure [Re: dumbfounded1600]
    #7977655 - 02/04/08 01:20 PM (15 years, 11 months ago)

r u serious


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InvisibleDay Tripper
Got a goodreason, for taking the easyway out.


Registered: 01/27/08
Posts: 198
Loc: Rocky Mountains
Re: Your Sterile Procedure [Re: dumbfounded1600]
    #7977656 - 02/04/08 01:20 PM (15 years, 11 months ago)

Wow, now that's comprehensive, I'll bet you are at maybe one contamed jar per year huh?

That was awesome, 5 shrooms for you man!


--------------------
Death is inevitable, and therefore irrelevant, life is optional, and therefore irreplaceable.


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InvisibleBEEP

Registered: 01/06/08
Posts: 1,385
Re: Your Sterile Procedure [Re: dumbfounded1600]
    #7977663 - 02/04/08 01:22 PM (15 years, 11 months ago)

a glove box would be more efficiant IMO then soaking the air in bleech.

i also cover my arms in saran wrap and soak them in 91% alchohol :headbanger:


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InvisibleDay Tripper
Got a goodreason, for taking the easyway out.


Registered: 01/27/08
Posts: 198
Loc: Rocky Mountains
Re: Your Sterile Procedure [Re: BEEP]
    #7977671 - 02/04/08 01:25 PM (15 years, 11 months ago)

Good call on the saran wrap, better than garbage bags...LOL...


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Offlineshevanel
Gone til November
 User Gallery


Registered: 01/27/08
Posts: 1,517
Last seen: 1 year, 11 months
Re: Your Sterile Procedure [Re: BEEP]
    #7977674 - 02/04/08 01:26 PM (15 years, 11 months ago)

i put my glovebox on a tv tray in front of the comode so I can poop while innoculating my jars...

No seriously, some of the thingsd in that method seem standard, but shaving your head? Spraying yourself with bleach water repeatedly?


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Invisibledumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
Re: Your Sterile Procedure [Re: shevanel]
    #7977684 - 02/04/08 01:30 PM (15 years, 11 months ago)

O and Glad Cling Wrap serves as a generic Parafilm


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Invisibledumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
Re: Your Sterile Procedure [Re: shevanel]
    #7977690 - 02/04/08 01:32 PM (15 years, 11 months ago)

Quote:

shevanel said:
i put my glovebox on a tv tray in front of the comode so I can poop while innoculating my jars...

No seriously, some of the thingsd in that method seem standard, but shaving your head? Spraying yourself with bleach water repeatedly?




I find no other method...that works...soon i'll be spraying myself with Clorox disinfectant


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Offlineshevanel
Gone til November
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Registered: 01/27/08
Posts: 1,517
Last seen: 1 year, 11 months
Re: Your Sterile Procedure [Re: dumbfounded1600]
    #7977693 - 02/04/08 01:33 PM (15 years, 11 months ago)

Ok, I believe you.

At first I thought you were being funny..


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Invisibledumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
Re: Your Sterile Procedure [Re: shevanel]
    #7977698 - 02/04/08 01:35 PM (15 years, 11 months ago)

No lie there...It's actually a Routine now


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Invisiblemycosis
Male User Gallery


Registered: 08/20/07
Posts: 19,727
Loc: USSA Flag
Re: Your Sterile Procedure [Re: shevanel]
    #7977702 - 02/04/08 01:36 PM (15 years, 11 months ago)

i needed a laugh like that...thanks!


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OfflineNeobean
Adept Mycologist
Male

Registered: 10/07/01
Posts: 975
Loc: Canada
Last seen: 3 years, 8 months
Re: Your Sterile Procedure [Re: shevanel]
    #7977707 - 02/04/08 01:37 PM (15 years, 11 months ago)

Quote:

shevanel said:
i put my glovebox on a tv tray in front of the comode so I can poop while innoculating my jars...

No seriously, some of the thingsd in that method seem standard, but shaving your head? Spraying yourself with bleach water repeatedly?




The dood is dedicated...5 shrooms!
:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:


--------------------
If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!


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Offlineshevanel
Gone til November
 User Gallery


Registered: 01/27/08
Posts: 1,517
Last seen: 1 year, 11 months
Re: Your Sterile Procedure [Re: Neobean]
    #7977716 - 02/04/08 01:39 PM (15 years, 11 months ago)

You make me feel inadequate and you give me day dreams of my freshly juiiced jars just sitting upstairs manufacturing mold of all types


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OfflineNeobean
Adept Mycologist
Male

Registered: 10/07/01
Posts: 975
Loc: Canada
Last seen: 3 years, 8 months
Re: Your Sterile Procedure [Re: shevanel]
    #7977749 - 02/04/08 01:50 PM (15 years, 11 months ago)

You can create the same environment in your glove box, don't be too worried :smile: This hobby, believe it or not, is not an all or nothing hobby. There is a middle ground, and a glove box is just that.


--------------------
If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!


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InvisibleDay Tripper
Got a goodreason, for taking the easyway out.


Registered: 01/27/08
Posts: 198
Loc: Rocky Mountains
Re: Your Sterile Procedure [Re: Neobean]
    #7977753 - 02/04/08 01:51 PM (15 years, 11 months ago)

He speaks the truth about the GB, they rock, so hard!


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Invisibledumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
Re: Your Sterile Procedure [Re: Day Tripper]
    #7977800 - 02/04/08 02:04 PM (15 years, 11 months ago)

Yes they do...I'd go with a laminar flow hood any day though


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OfflineiTrip
Electronic Trip
Male


Registered: 11/21/07
Posts: 68
Last seen: 14 years, 6 months
Re: Your Sterile Procedure [Re: shevanel]
    #7977883 - 02/04/08 02:29 PM (15 years, 11 months ago)

DAMN! thats way too much work for my lazy ass... :cool:

i actually use my oven.. turn it to the lowest setting possible

when it starts to get hot enough i place my jars on the bottom  shelf and procced with normal INNOC>. and since ive been using this method i havent gotten 1 single contam jar!

Also a lit candle can provide 1-2 sq feet of sterile air space :eek:


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Invisibledumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
Re: Your Sterile Procedure [Re: iTrip]
    #7977901 - 02/04/08 02:33 PM (15 years, 11 months ago)

Quote:

iTrip said:
DAMN! thats way too much work for my lazy ass... :cool:

i actually use my oven.. turn it to the lowest setting possible

when it starts to get hot enough i place my jars on the bottom  shelf and procced with normal INNOC>. and since ive been using this method i havent gotten 1 single contam jar!

Also a lit candle can provide 1-2 sq feet of sterile air space :eek:




Ovens suck unless you can crawl in there yourself to inoculate...and a lit candle? *COUGH* sorry....I was coughing on the bullshit...they'll actually try to surface more because there attracted to heat.


Edited by dumbfounded1600 (02/04/08 02:34 PM)


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OfflineiTrip
Electronic Trip
Male


Registered: 11/21/07
Posts: 68
Last seen: 14 years, 6 months
Re: Your Sterile Procedure [Re: dumbfounded1600]
    #7977928 - 02/04/08 02:42 PM (15 years, 11 months ago)

Quote:

dumbfounded1600 said:
Quote:

iTrip said:
DAMN! thats way too much work for my lazy ass... :cool:

i actually use my oven.. turn it to the lowest setting possible

when it starts to get hot enough i place my jars on the bottom  shelf and procced with normal INNOC>. and since ive been using this method i havent gotten 1 single contam jar!

Also a lit candle can provide 1-2 sq feet of sterile air space :eek:




Ovens suck unless you can crawl in there yourself to inoculate...and a lit candle? *COUGH* sorry....I was coughing on the bullshit...they'll actually try to surface more because there attracted to heat.




well its worked for me.! ive had no contamd jars since i started using this method.!


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OfflineP.Menace
Contradicting the Status Quo
Male User Gallery


Folding@home Statistics
Registered: 11/17/07
Posts: 4,797
Loc: Let Me See Potato Salad!!...
Last seen: 9 years, 10 months
Trusted Cultivator
Re: Your Sterile Procedure [Re: Day Tripper]
    #7977937 - 02/04/08 02:46 PM (15 years, 11 months ago)

Get a flow hood or glove box! I just use a cardboard box with a window cut in the top and covered with a picture frame glass. and a hole big enough to slip in a dozen Qt jars in the box the jars came in. I throw in a light when Im going to use it, Nuke the entire thing with Lysol, Oust, Clorox, whatever I have, I use hand sanitizer always, latex gloves when I have them. 100% success. well unless you count the 1 jar that may have been missed or there was no mycelium in the syringe when inoculated, But for 181 jars Thats great. Plus it proves my practices by showing capability to produce many many many clean viable LC's from clone , SS, SP and previous LC's.


--------------------

http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace


roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.


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Shop: Mushroom-Hut Liquid Cultures, Substrate Mix   North Spore Bulk Substrate, Injection Grain Bag


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