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Offlineiknowyourider
Stranger
Registered: 02/03/08
Posts: 10
Last seen: 15 years, 8 months
Should I hit the restart button?
    #7975309 - 02/03/08 09:03 PM (15 years, 11 months ago)

Hello all, this is my first post on the boards, and I'm looking for a bit of advice for my first grow:

I inoculated 11 BRF cakes on January 24 with B+ from sporelab.com and have since been incubating them using the tub in tub method with an aquarium heater at 80degrees F. I made my substrate by mixing 4 cups verm, 2 cups BRF, and 2 cups water and sterilized in a pressure cooker for aprox. 1 hour. The jars were cooled overnight before inoculation.
Mycelium is only visible on two of the jars (one jar shows growth at 2 inoc. points and the other jar only shows growth at one point of injection).

Is there an obvious answer as to why mycelium has failed to form in the other jars? I was very sterile throughout the process and my syringe was heated with an alcohol lamp between injecting different jars. The only thing I was worried about was that when I went to inject after heating the needle, liquid/steam sputtered out of the needle for a second from being so hot I suppose until it was cooled. Could that have possibly killed the spores and could I just sterilize the needle with ethyl or isopropyl alcohol instead? Is it possible that there were only a few spores present in the liquid and they therefore were only injected into the two jars?

Also water droplets are visible on the inside of the jars so I think that the water content may have been altered during time in the pressure cooker. The jars were only sealed with lids and covered with aluminum foil.

I am a bit discouraged because I felt that I did everything correctly. Please offer any insight or help. I am planning on scrapping the 9 dud jars are having another go with another syringe of Cams I have, unless I get some advice to do otherwise. Thanks


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Offlinelovingtickels
wit a engineringdegre wo neds tono how 2 spel


Registered: 04/10/07
Posts: 220
Loc: North or South America
Last seen: 11 years, 10 months
Re: Should I hit the restart button? [Re: iknowyourider]
    #7975331 - 02/03/08 09:07 PM (15 years, 11 months ago)

If all you see is "some" myc growth. you are successful in my opion. for now i would only worry if you see something else grow. congrats and have patience. my first pftek took a month to colonize because of over compaction of the material, but it colonized eventually. patience my friend


--------------------
I never guess. It is a capital mistake to theorize before one has data. Insensibly one begins to twist facts to suit theories, instead of theories to suit facts.
Sir Arthur Conan Doyle

Mistakes are a part of being human. Appreciate your mistakes for what they are: precious life lessons that can only be learned the hard way. Unless it's a fatal mistake, which, at least, others can learn from.
- Al Franken


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Invisiblethedefone
deus ex machina

Registered: 10/06/07
Posts: 1,883
Loc: Gondwana
Re: Should I hit the restart button? [Re: iknowyourider]
    #7975345 - 02/03/08 09:10 PM (15 years, 11 months ago)

Sounds like you did things right, and are not an idiot. Wait another week, and if you still see no growth in your remaining jars, try again.

What temp do you have your jars at? The sputtering needle is normal, but did you cool the needle with an alcohol swab before injection? The water droplets on the jar's interior is normal, however, you should be able to easily tell if the water content was jacked up during PC.

Wait on the jars you already knocked up, while starting a new grow with that other syringe. Good luck.


--------------------


I am become death, the destroyer of worlds.


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Offlineiknowyourider
Stranger
Registered: 02/03/08
Posts: 10
Last seen: 15 years, 8 months
Re: Should I hit the restart button? [Re: lovingtickels]
    #7975358 - 02/03/08 09:12 PM (15 years, 11 months ago)

so it is possible it may take longer than 10 days for myc. to show up on the other jars? I dont know if too much water in the jars will cause no myc. to form.


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Offlinelovingtickels
wit a engineringdegre wo neds tono how 2 spel


Registered: 04/10/07
Posts: 220
Loc: North or South America
Last seen: 11 years, 10 months
Re: Should I hit the restart button? [Re: iknowyourider]
    #7975404 - 02/03/08 09:19 PM (15 years, 11 months ago)

I rarely use the pftek. when i do, i rarley have all points display growth. but as the above post suggests. lets look at other factors. what is your incubation temperature? and about the alcohol swab after heating- i don't do it. i just squirt a little out before i insert it. i'm too scared that a swab might still harbor some contaminants and after it is red hot i know it is sterile. waste some juice to cool it. just me though. the guy that swabs it knows more then me from what i can tell.


--------------------
I never guess. It is a capital mistake to theorize before one has data. Insensibly one begins to twist facts to suit theories, instead of theories to suit facts.
Sir Arthur Conan Doyle

Mistakes are a part of being human. Appreciate your mistakes for what they are: precious life lessons that can only be learned the hard way. Unless it's a fatal mistake, which, at least, others can learn from.
- Al Franken


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Offlineiknowyourider
Stranger
Registered: 02/03/08
Posts: 10
Last seen: 15 years, 8 months
Re: Should I hit the restart button? [Re: lovingtickels]
    #7975420 - 02/03/08 09:24 PM (15 years, 11 months ago)

incubation temperature is ~80 degrees, with a 10 day high of 83 and a low of 73 because it tends to get pretty cold in my house at night (tryin to save some money on the heating bill).


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Offlinelovingtickels
wit a engineringdegre wo neds tono how 2 spel


Registered: 04/10/07
Posts: 220
Loc: North or South America
Last seen: 11 years, 10 months
Re: Should I hit the restart button? [Re: iknowyourider]
    #7975437 - 02/03/08 09:28 PM (15 years, 11 months ago)

your fine, congrats and have patience, update us in five days and every days after that


--------------------
I never guess. It is a capital mistake to theorize before one has data. Insensibly one begins to twist facts to suit theories, instead of theories to suit facts.
Sir Arthur Conan Doyle

Mistakes are a part of being human. Appreciate your mistakes for what they are: precious life lessons that can only be learned the hard way. Unless it's a fatal mistake, which, at least, others can learn from.
- Al Franken


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Offlineiknowyourider
Stranger
Registered: 02/03/08
Posts: 10
Last seen: 15 years, 8 months
Re: Should I hit the restart button? [Re: lovingtickels]
    #8007269 - 02/11/08 12:27 AM (15 years, 11 months ago)

So just an update on my first grow status...and i need some advice.

2 of 11 are still colonizing nicely...but the other 9 havent begun colonizing at all. im baffled as to why a couple have worked, yet the majority show no signs of mycelium at all. spores from sporelab.com...are they reputable?

should i scrap the 9 "duds" at this point and retry with another syringe, or can it take longer than 19-20 days for the first signs of mycelium to appear in a jar?


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OfflineP.Menace
Contradicting the Status Quo
Male User Gallery


Folding@home Statistics
Registered: 11/17/07
Posts: 4,797
Loc: Let Me See Potato Salad!!...
Last seen: 9 years, 10 months
Trusted Cultivator
Re: Should I hit the restart button? [Re: iknowyourider]
    #8007411 - 02/11/08 01:20 AM (15 years, 11 months ago)

I had a jar take over a month to germinate, 3 other jars in the batch of 4 contaminated and the 1 got forgotten about and set on the radiator for a few days before I realized it, out of the blue, it had mycellium growing in 2 places, It then took almost another month to fully colonize, and Last week actually I Picked the 1 average sized muhsie off of it.
In case you are wondering, the spores were 20 Years give or take old! They came from an OLD baggie in which someone had purchased Shrooms In, they ate the shrooms and placed the baggie in a picture frame behind a picture and forgot about it, until they wanted to change up that certain pic, found the baggie gave it to me, I filled it with sterile water, let it sit for a few days and used it as innoculant. all before my first syringes showed up in the mail


--------------------

http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace


roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.


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