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OfflineSamurai_Jim
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Green mold in colonized rye jar???
    #7975074 - 02/03/08 08:19 PM (15 years, 11 months ago)

SO one of my jars, with a three layer tyvek lid, was 70% colonized with myc. I looked at it last night and it was fine. Today, about 4pm, I went into my grow room to find the top 3" or so of the grain swamped with green mold? WTF happened? I did not open the jar, everything was going fine.

Also one of my PF cakes had green mold in it too. My PF cakes have 3 layer tyvek lids with self healing injection ports. I PCed @ 15 psi for 40 mins.


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Decreasing the world's bug population, one faceshield at a time.


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Invisiblethedefone
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Registered: 10/06/07
Posts: 1,883
Loc: Gondwana
Re: Green mold in colonized rye jar??? [Re: Samurai_Jim]
    #7975137 - 02/03/08 08:31 PM (15 years, 11 months ago)

It contaminated. Sorry. Shit happens, and you just have to deal with it. Clean up, and re-evaluate. Good Luck!


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OfflineRogerRabbitM
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Re: Green mold in colonized rye jar??? [Re: thedefone]
    #7975179 - 02/03/08 08:39 PM (15 years, 11 months ago)

Trichoderma mycelium is white, so while you looked at them last night, all was not fine. Today, the mold sporulated.

I'm sure the mold was introduced at inoculation, provided your filters and lids are sound.
RR


--------------------
Download Let's Grow Mushrooms



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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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OfflineSamurai_Jim
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Re: Green mold in colonized rye jar??? [Re: RogerRabbit]
    #7975194 - 02/03/08 08:41 PM (15 years, 11 months ago)

I should just skip all of this trying to innoc mith spores and go agar. I can isolate it from there.

I have some of these little petri dishes that are like 35mm x 10mm. Can I use them or are they too small?


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Decreasing the world's bug population, one faceshield at a time.


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Invisiblethedefone
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Re: Green mold in colonized rye jar??? [Re: Samurai_Jim]
    #7975252 - 02/03/08 08:52 PM (15 years, 11 months ago)

Do you have a flow hood? If not, then it will be very hard. Look into clone LC's. That way at least you can replicate the traits you want, but not as specifically as you would be able to do with agar.


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I am become death, the destroyer of worlds.


Edited by thedefone (02/03/08 08:52 PM)


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Invisibletahoe
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Re: Green mold in colonized rye jar??? [Re: Samurai_Jim]
    #7975266 - 02/03/08 08:54 PM (15 years, 11 months ago)

Quote:

Samurai_Jim said:
I should just skip all of this trying to innoc mith spores and go agar. I can isolate it from there.

I have some of these little petri dishes that are like 35mm x 10mm. Can I use them or are they too small?



You can use those but they are garbage. I have a bunch of pyrex ones that size.

Spore syringes work great if they are clean. Did you simmer the rye and cook it fully? 3 tyvek lids is over kill. I use tyvek with the metal rim. I inoculate right through the tyvek and cover the hole with masking tape and cover the top with aluminum foil afterwards


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


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OfflineSamurai_Jim
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Re: Green mold in colonized rye jar??? [Re: tahoe]
    #7975482 - 02/03/08 09:37 PM (15 years, 11 months ago)

Quote:

tahoe said:
You can use those but they are garbage. I have a bunch of pyrex ones that size.

Spore syringes work great if they are clean. Did you simmer the rye and cook it fully? 3 tyvek lids is over kill. I use tyvek with the metal rim. I inoculate right through the tyvek and cover the hole with masking tape and cover the top with aluminum foil afterwards




Well, I didn't soak and simmer these, I wanted to try the "in a pinch" method of washing, jarring with water and PCing. I am soaking 10 cups right now. Been soaking for 26 hours. I'll be canning and PCing tomorrow. So you are saying I should just use 1 layer of tyvek over the metal lid? I have lids that have 5 holes in them. 1 hole has silicone on it for self healing injection port. the other 4 are for gas exchange. Can I use those?

Also I have tried LC's from spore before and they never turned out. Always had bactiria. Is it really wise to try and start a LC from spore? THat is what I want to use the petri dishes for. Getting some myc going and then transfer to grain or LC.

I just got a large hepa filter for my grow room. That should help me on cutting down some of the contam. Between oust and the hepa, I should be a bit more safe, right? Also, I use a glovebox for grain prep and G2G, I CAN use that with the agar with success, right?


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Invisiblethedefone
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Re: Green mold in colonized rye jar??? [Re: Samurai_Jim]
    #7975590 - 02/03/08 10:03 PM (15 years, 11 months ago)

Quote:

Also I have tried LC's from spore before and they never turned out. Always had bactiria. Is it really wise to try and start a LC from spore?



That's why I said do a clone LC. Take a chunk from the inside of the stem of a fruit you like, and put that in a LC while working in a glove box. Voila.

Quote:

Also, I use a glovebox for grain prep and G2G, I CAN use that with the agar with success, right?



I am just telling you what I have read. Agar work is difficult without a flow hood.


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OfflineSamurai_Jim
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Re: Green mold in colonized rye jar??? [Re: thedefone]
    #7975828 - 02/03/08 10:55 PM (15 years, 11 months ago)

Well, I have never did any agar work without a flow hood. What little I did was under a flow hood at school. But I don't even have access to anything of that type anymore.

When I use a glovebox, I put all of the items in the box and then fume with oust from an access hole. After about 15 mins I start to work. I just adopted this principle about a week ago, and I don't know how affective it is yet, as nothing I knocked up has started to grow yet. I'll see soon.

I can make a flow hood, but I don't have a blower. I have a 24x24x3 TRUE hepa filter just waiting.


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OfflineNeobean
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Re: Green mold in colonized rye jar??? [Re: Samurai_Jim]
    #7976971 - 02/04/08 09:17 AM (15 years, 11 months ago)

Quote:

Samurai_Jim said:
Well, I have never did any agar work without a flow hood. What little I did was under a flow hood at school. But I don't even have access to anything of that type anymore.

When I use a glovebox, I put all of the items in the box and then fume with oust from an access hole. After about 15 mins I start to work. I just adopted this principle about a week ago, and I don't know how affective it is yet, as nothing I knocked up has started to grow yet. I'll see soon.

I can make a flow hood, but I don't have a blower. I have a 24x24x3 TRUE hepa filter just waiting.




You're good with your glove box, just keep it clean.

Also I read up there that you PC for 40 minutes. I doubt that is the cause in this situation, but you might want to PC for longer than that.


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OfflineRogerRabbitM
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Re: Green mold in colonized rye jar??? [Re: Neobean]
    #7977015 - 02/04/08 09:31 AM (15 years, 11 months ago)

I pc 1/2 pint cakes for an hour, and 1 quart jars of rye for 90 minutes. However, this is to prevent bacteria. If green mold is the problem, you can take it to the bank that it was intrduced at inoculation.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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InvisibleDay Tripper
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Registered: 01/27/08
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Re: Green mold in colonized rye jar??? [Re: RogerRabbit]
    #7977171 - 02/04/08 10:28 AM (15 years, 11 months ago)

Try G2G in a glovebox man, many report 99% success, myself included.

Plus, multi-spore is only good to get started, never continue to use spores in jars, IMHO.

You can cut a slice off a good fruit and drop it into a grain jar for the most simple clone method possible.

Or go the agar route, and isolate, then grow out each strain.

Honestly, every time I see a great fruit staring I pick it, and cut a few slices off for rye jars.

In a glovebox you can get near perfect accuracy, but if 1-3 contam in a pint jar, no worries as long as you got one good culture from your clone.


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