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OfflineSamurai_Jim
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Registered: 01/12/08
Posts: 435
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How is it possible that I contaminated the LC?
    #7971072 - 02/02/08 10:38 PM (15 years, 11 months ago)

Well, I am known to do favors, and I was doing one for a close friend. I was making him some LC jars, and was going to let him take it from there.

I made 5 jars. Soake dthe jars in bleach water for 15 mins, rinsed off and put 500ml of water in them. I used Barley Malt and spring water. 1/4 tsp of malt to 500ml water. Tyvek lids with self healing injection port. Wiped jar rim with alcohol soaked cloth. PCed @ 15 psi for 25 mins. When I injected the spores, I used a propane torch, and alcohol soaked cloth. Wiped the injection port, flamed the needle and then straight onto the alcohol soaked cloth. From there I uncovered the injection port and injected 2cc of spore solution.

2.5 days later and there isn't much growth, but the LC has turned a bit cloudy. I can still see through it, but it isn't as clear as it was when it came out of the PCer.

How the heck could I have screwed this up?


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Decreasing the world's bug population, one faceshield at a time.


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Invisibletahoe
Noob Slayer
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Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: How is it possible that I contaminated the LC? [Re: Samurai_Jim]
    #7971132 - 02/02/08 10:54 PM (15 years, 11 months ago)

tyvek has no place with lc's. Spore syringes suck when it comes to lc's. Why would you remove the injection port?


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Invisiblesandman420
Saint PP
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Registered: 06/17/04
Posts: 5,384
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Re: How is it possible that I contaminated the LC? [Re: tahoe]
    #7971138 - 02/02/08 10:57 PM (15 years, 11 months ago)

what do you mean spore syringes suck when it comes to lc? And tyvek can make a decent air filter port for an lc jar, why wouldnt it? As long as its not getting wet.


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- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


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OfflineSamurai_Jim
Samurai Emperor.
Male

Registered: 01/12/08
Posts: 435
Loc: Near the Tail of the Drag...
Last seen: 1 year, 8 months
Re: How is it possible that I contaminated the LC? [Re: tahoe]
    #7971144 - 02/02/08 10:58 PM (15 years, 11 months ago)

I didn't remove the injection port. I had an alcohol soaked cloth over top of the lid, I lifted the cloth as I was ready to inject the spores.

Spores have worked for me before with LC, took longer, but it worked.


--------------------
Decreasing the world's bug population, one faceshield at a time.


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Invisibletahoe
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Re: How is it possible that I contaminated the LC? [Re: sandman420]
    #7971156 - 02/02/08 11:03 PM (15 years, 11 months ago)

Quote:

sandman420 said:
what do you mean spore syringes suck when it comes to lc? And tyvek can make a decent air filter port for an lc jar, why wouldnt it? As long as its not getting wet.




I didnt say tyvek doesnt work for lcs. I said it sucks and that is the exact reason.

spore syringe suck for lc's. Lets say tehre are 100 bad mold spores in a ss. You use that ss for a pf jar. you squirt 10 bad mold spores and 1000's of cube spores in the same hole. the 10 bad spores will most likely get over taken by the cubes and never be a problem.

Now you do the same for a lc. thos 1000 mushroom spores germinated and grow. the mold spores do the same because they can float around.
In a pf jar everything stays still. The spores stay put, they have to compete.

In a lc the spores float around and have all teh food they want


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Invisiblesandman420
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Registered: 06/17/04
Posts: 5,384
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Re: How is it possible that I contaminated the LC? [Re: tahoe]
    #7971182 - 02/02/08 11:10 PM (15 years, 11 months ago)

so what do you recomend for

A) Filter material for LCs
B) inoculation method of LCs if starting from spores?


Ive been using tyvek sleeve material cut into small 1/2" squares siliconed to the top of the lid over a 1/8th " hole and also another rtv silicone port for syringe. Works good with a magnetic stir bar set up. When its time to draw LC you just make sure to turn the lc on its side very carfully to keep the liquid out of the filter its pretty easy. No need for an airport syringe that way. Leave jar rings loose when pcing and the tyvek never gets wet and the lc finishes in 4-7 days anyways.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


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Invisibletahoe
Noob Slayer
Male User Gallery


Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
Trusted Cultivator
Re: How is it possible that I contaminated the LC? [Re: sandman420]
    #7971224 - 02/02/08 11:21 PM (15 years, 11 months ago)

I would recomend the lid design that agar came up with.
If not than a plastic lid with an injection port and polyfill. If polyfill gets wet it will most likely not contam. If tyvek gets wet it will most likely contams.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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