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sandman420
Saint PP


Registered: 06/17/04
Posts: 5,384
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Mac 10 Self Powered Hepa as a flowhood?
#7968486 - 02/02/08 12:29 PM (15 years, 11 months ago) |
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Do you think that a 2'x2' Envirco "Mac 10" turned on its side so its blowing horizontally would make an effective flowhood for some agar work?
http://www.envirco.com/PDF/Mac10_Original.pdf
its basically just a self powered 99.99% hepa that blows about 90fpm or so I think.
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Skeeblix
Dave Thomas



Registered: 01/28/07 
Posts: 1,745
Loc: Wendy's
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Re: Mac 10 Self Powered Hepa as a flowhood? [Re: sandman420]
#7968519 - 02/02/08 12:36 PM (15 years, 11 months ago) |
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EDIT: okay, I looked in a little more detail at the pdf you supplied, and it looks kind of promising for you.
Considering I'm not entirely sure its producing laminar flow, the only way to know is for you to check yourself.
There's a lot of people who know more about flow hoods than I do who can tell you how to do this, namely RR, since he's assisted countless members with flowhood construction and all that.
-------------------- This post approved by:
Premedman1 said:
I just shat my pants.
Edited by Skeeblix (02/02/08 12:41 PM)
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Skeeblix
Dave Thomas



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Re: Mac 10 Self Powered Hepa as a flowhood? [Re: Skeeblix]
#7968561 - 02/02/08 12:46 PM (15 years, 11 months ago) |
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Well, okay, I've googled around and you're good to go. That thing DOES output laminar flow, and from what I've read its used in clean rooms and hospitals where sterility is key.
Give it a testing, if it's not suitable for the job, your first set of plates will more than likely show it.
EDIT: How the HELL did you come across something like this, anyways?
-------------------- This post approved by:
Premedman1 said:
I just shat my pants.
Edited by Skeeblix (02/02/08 12:47 PM)
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sandman420
Saint PP


Registered: 06/17/04
Posts: 5,384
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Re: Mac 10 Self Powered Hepa as a flowhood? [Re: Skeeblix]
#7968699 - 02/02/08 01:16 PM (15 years, 11 months ago) |
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hehe, the interwebs are full of cheap thingies if your lucky and persistant. Got it for about $200 shipped to me in new condition! Its been hanging from the ceiling for a few months but i dont ever run it really but for 15 mins at a time every week or so. Im thinking of ripping it down and using it for a flowhood now...sice i tore my old flowhood up by trying to move it in a fussy mood with my girlfriend and i broke it
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Re: Mac 10 Self Powered Hepa as a flowhood? [Re: sandman420]
#7969361 - 02/02/08 04:10 PM (15 years, 11 months ago) |
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It's not going to flow as much as a flowhood normally should, but might still work OK if you do your work right up agaist it. It's going to flow at an airspeed of about 80-90 feet per minute, while 100 to 120 feet per minute is recommended. Note it's output is 360 cubic feet per minute spread over 4 square feet.
For flowhood use, I'd let it run in a small room at least an hour before beginning work in order to scrub the ambient air clean. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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sandman420
Saint PP


Registered: 06/17/04
Posts: 5,384
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Re: Mac 10 Self Powered Hepa as a flowhood? [Re: RogerRabbit]
#7969907 - 02/02/08 06:30 PM (15 years, 11 months ago) |
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im testin' it out here in a second. I dont really have a small room to do it in conveniently and the bathroom wouldnt work so im hoping it works in the kitchenette area...



doing up about 400ml of agar from sporeworks to pour a few plates and see what happens. Its cooking now! WEeeeeeeeeeeeeeeeEEEee!!!(squirrel noise)
Notice my fancy Plexiglases on top and bottom to give a small area to clean with extra fancy anti-germicidal... Hope it works!
Edited by sandman420 (02/02/08 06:34 PM)
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orchidfanatic
retiree




Registered: 08/12/07
Posts: 832
Loc: where the wild things are
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Re: Mac 10 Self Powered Hepa as a flowhood? [Re: sandman420]
#7969921 - 02/02/08 06:35 PM (15 years, 11 months ago) |
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good luck with it .. I poured 8 plates in front of my flow hood for yeast work and 2 showed contams .. so I will run it for a longer period before starting work with it . let us know how to goes..
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sandman420
Saint PP


Registered: 06/17/04
Posts: 5,384
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Re: Mac 10 Self Powered Hepa as a flowhood? [Re: orchidfanatic]
#7969923 - 02/02/08 06:36 PM (15 years, 11 months ago) |
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how long until you saw contams?
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
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Re: Mac 10 Self Powered Hepa as a flowhood? [Re: sandman420]
#7970352 - 02/02/08 08:04 PM (15 years, 11 months ago) |
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I'd put up some dividers around your work area to stop drafts. A weak flowhood like that can easily have the sterile flow overcome by drafts in the room. The reason for the small room is so the flowhood can scrub the air clean in the hour or two you let it run prior to starting work.
If the face of it is ceramic, you can spray it with lysol before turning on to kill contaminants that have come to rest on the surface of the filter itself. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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sandman420
Saint PP


Registered: 06/17/04
Posts: 5,384
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Re: Mac 10 Self Powered Hepa as a flowhood? [Re: sandman420]
#7970369 - 02/02/08 08:08 PM (15 years, 11 months ago) |
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alright i did it! I shot 5 cc of peroxide into each flask of agar which had about 200ml inside or so. THen swirled and poured some plates.

In good roger rabit fashion (I didnt want to make you too upset by not using in an enclosed space) I also did a few "hot agar sandwhich" plates in which i poured the hot agar in the plate on a little chunk of agar i cut from a dish that had condensated all over itself in the fridge and is surely contammed from the water being on it even though it apears fine (been refreidgerated for a while). I made a few lcs from similar agar plates that had condensated and they contammed.... hopefully the good will grow through the top and the baddies will stay submerged.
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orchidfanatic
retiree




Registered: 08/12/07
Posts: 832
Loc: where the wild things are
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Re: Mac 10 Self Powered Hepa as a flowhood? [Re: sandman420]
#7972133 - 02/03/08 09:21 AM (15 years, 11 months ago) |
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Quote:
sandman420 said: how long until you saw contams?
about 4-5 days .. I was just testing the flow hood I poured them and put them inside a tupperware container sealed and let them sit .. to see how many contaminated 2 out of 8 in my dusty garage aint bad..
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spizaetus
Stranger
Registered: 12/23/17
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Last seen: 6 years, 9 days
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Re: Mac 10 Self Powered Hepa as a flowhood? [Re: sandman420]
#24863298 - 12/23/17 06:38 AM (6 years, 1 month ago) |
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Did it work? Are you happy with your envirco Mac 10? Thanks.
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Fungus Mountain
Poke-N-Squirt



Registered: 10/16/14
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Loc: Front row @ the Freakshow
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Re: Mac 10 Self Powered Hepa as a flowhood? [Re: spizaetus]
#24863302 - 12/23/17 06:41 AM (6 years, 1 month ago) |
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10 years man. You just asked a question on a 10 year old thread.
Welcome!
-------------------- “Until they became conscious they will never rebel, and until after they have rebelled they cannot become conscious.” ― George Orwell, 1984 "If you can't explain it simply, you don't understand it well enough." ― Albert Einstein
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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No it didnt work very good
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Zachsonpub
Friend



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Re: Mac 10 Self Powered Hepa as a flowhood? [Re: sandman420]
#24863527 - 12/23/17 09:54 AM (6 years, 1 month ago) |
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Wow, quick response regardless...
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Mac 10 Self Powered Hepa as a flowhood? [Re: Zachsonpub]
#24863530 - 12/23/17 09:57 AM (6 years, 1 month ago) |
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First time OP of a 10 year old thread still happens to be around lol.
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Variegated
Pressure cooker operator



Registered: 01/15/17
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Re: Mac 10 Self Powered Hepa as a flowhood? [Re: bodhisatta]
#24863581 - 12/23/17 10:40 AM (6 years, 1 month ago) |
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I had to read it twice just to make sure i wasnt seeing stuff..op still here..wow
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spizaetus
Stranger
Registered: 12/23/17
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Last seen: 6 years, 9 days
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Re: Mac 10 Self Powered Hepa as a flowhood? [Re: sandman420]
#24863664 - 12/23/17 11:26 AM (6 years, 1 month ago) |
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Thank you for your prompt response. I am thinking to buy a flow hood, any recommendation? Have a great weekend.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Mac 10 Self Powered Hepa as a flowhood? [Re: bodhisatta]
#24863671 - 12/23/17 11:31 AM (6 years, 1 month ago) |
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Quote:
bodhisatta said: First time OP of a 10 year old thread still happens to be around lol.
I'm so impressed. Can't even be mad.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Mac 10 Self Powered Hepa as a flowhood? [Re: van hatton]
#24863920 - 12/23/17 01:22 PM (6 years, 1 month ago) |
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I see a used Baker edgeGARD IV-22 on ebay for $62.99 located in Colorado. I'd jump on that if I lived close by or could afford 300+ shipping on top. I'm sure it would need a new filter too though. 24x24x5-7/8 99.99 hepa with a .7" WG @ 500cfm with downstream gasket is the replacement stats for the filter.
Edited by sandman420 (12/23/17 01:29 PM)
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