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zero cool
I'm a bag kindaguy



Registered: 09/13/07
Posts: 250
Last seen: 15 years, 8 months
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needles and syringes from spores101...
#7966143 - 02/01/08 06:37 PM (16 years, 4 hours ago) |
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ok, i just got some syringes from spores101 and they came with the needle not attached to the syringe. so my question is...how do i put the needle on the syringe without letting any contams get inside the needle. the last time i tried this, all my projects were contamed! i guess i should just flame sterilize and use alcohol too...but will this also sterilize the plastic part where the needle screws into the syringe? and i also want to know if there is a company that sends the needles already attached to the syringes...i have been having a problem with contams lately any help is appreciated. thanks
-------------------- git them mushrooms Mario! git em!
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The shroomy 1
Luminous beings surround me




Registered: 03/27/07
Posts: 5,543
Loc: The Aether
Last seen: 5 months, 5 days
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Re: needles and syringes from spores101... [Re: zero cool]
#7966177 - 02/01/08 06:55 PM (16 years, 4 hours ago) |
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LOL! I've never received a syringe that was attached already. The needle is "sealed". The way I go about this is that I have a medium size tupperware type container, (one that will hold the length of the syringe). I fill it with alcohol about half way, and drop the needle and syringe in it. Let it sit there for a few minutes while you get everything else in order. I use a glove box for inoculations. So once everything is in the glove box and ready for inoculation I take the needle and syringe out of the alcohol bath and put my hands (with needle and syringe) through the holes of the glove box. Once inside the glove box, I take off the syringe cap/stopper and screw on the needle. Pop off the sealed needle sheath and your ready to go.
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AMU Q&A thread.
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zero cool
I'm a bag kindaguy



Registered: 09/13/07
Posts: 250
Last seen: 15 years, 8 months
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Re: needles and syringes from spores101... [Re: The shroomy 1]
#7966210 - 02/01/08 07:03 PM (16 years, 4 hours ago) |
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hmmm...so even if you get alcohol inside the needle it's ok? will alcohol kill spores? and i remember getting syringes with needle attached from two different companies...i cant remember if they were sponsored by the shroomery, but they workd tho.
-------------------- git them mushrooms Mario! git em!
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The shroomy 1
Luminous beings surround me




Registered: 03/27/07
Posts: 5,543
Loc: The Aether
Last seen: 5 months, 5 days
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Re: needles and syringes from spores101... [Re: zero cool]
#7966224 - 02/01/08 07:08 PM (16 years, 4 hours ago) |
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Remember, you have to flame the needle. That will get rid of the alcohol. The Sheath is sealed to prevent contamination so alcohol shouldn't even be getting in to begin with. It's the way I've done it time and time again. I've never had a problem with it affecting the spores in the syringe.
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AMU Q&A thread.
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zero cool
I'm a bag kindaguy



Registered: 09/13/07
Posts: 250
Last seen: 15 years, 8 months
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Re: needles and syringes from spores101... [Re: The shroomy 1]
#7966234 - 02/01/08 07:12 PM (16 years, 4 hours ago) |
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thanks for the advice man, hopefully all goes well this time...
-------------------- git them mushrooms Mario! git em!
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The shroomy 1
Luminous beings surround me




Registered: 03/27/07
Posts: 5,543
Loc: The Aether
Last seen: 5 months, 5 days
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Re: needles and syringes from spores101... [Re: zero cool]
#7966252 - 02/01/08 07:22 PM (16 years, 4 hours ago) |
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No problem, GOOD LUCK!
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AMU Q&A thread.
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: needles and syringes from spores101... [Re: zero cool]
#7966289 - 02/01/08 07:33 PM (16 years, 3 hours ago) |
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Quote:
zero cool said: ok, i just got some syringes from spores101 and they came with the needle not attached to the syringe. so my question is...how do i put the needle on the syringe without letting any contams get inside the needle. the last time i tried this, all my projects were contamed!
There was something else wrong. There is no way that you would have screwed everything up by putting on the needle unless you just dug through the trash with your bare hands and then fully removed teh needle from the plastic sleeve and rubbed the needle on your hands and then stuck it in the trash and then put it on.
It isnt that hard to do. Something else went wrong although spore101 has put out some dirty syringes
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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Knots_Landing
You're a towel!


Registered: 02/01/08
Posts: 13
Last seen: 15 years, 3 months
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Re: needles and syringes from spores101... [Re: The shroomy 1]
#7966291 - 02/01/08 07:34 PM (16 years, 3 hours ago) |
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The Hawk sends syringes w/ needles already attached. www.thehawkseye.com
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: needles and syringes from spores101... [Re: Knots_Landing]
#7966302 - 02/01/08 07:36 PM (16 years, 3 hours ago) |
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great first post. It isnt that difficult. It should be idiot proof but you guys continue to prove to me that nothing is idiot proof
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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Samurai_Jim
Samurai Emperor.


Registered: 01/12/08
Posts: 435
Loc: Near the Tail of the Drag...
Last seen: 1 year, 8 months
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Re: needles and syringes from spores101... [Re: tahoe]
#7966493 - 02/01/08 08:26 PM (16 years, 3 hours ago) |
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Ha!, I ordered about $150 of syringes from spores101, I got my order and two weeks later I got another IDENTICAL order. 2 for the price of 1. Not bad huh?
They were kind of slow though.
-------------------- Decreasing the world's bug population, one faceshield at a time.
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The shroomy 1
Luminous beings surround me




Registered: 03/27/07
Posts: 5,543
Loc: The Aether
Last seen: 5 months, 5 days
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Re: needles and syringes from spores101... [Re: Samurai_Jim]
#7966878 - 02/01/08 09:54 PM (16 years, 1 hour ago) |
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Quote:
Samurai_Jim said: Ha!, I ordered about $150 of syringes from spores101, I got my order and two weeks later I got another IDENTICAL order. 2 for the price of 1. Not bad huh?
They were kind of slow though.
Did you thank them?;)
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AMU Q&A thread.
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emrandel
Love iS freeD


Registered: 03/03/07
Posts: 94
Last seen: 15 years, 9 months
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Re: needles and syringes from spores101... [Re: The shroomy 1]
#7966929 - 02/01/08 10:09 PM (16 years, 1 hour ago) |
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I've ALWAYS gotten my syringes with the needles attached. From hawk and lilshopofspores.
-------------------- "No drug, not even alcohol, causes the fundamental ills of society. If we're looking for the source of our troubles, we shouldn't test people for drugs, we should test them for stupidity, ignorance, green and love of power!" - P.J. O'Rourke
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zero cool
I'm a bag kindaguy



Registered: 09/13/07
Posts: 250
Last seen: 15 years, 8 months
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Re: needles and syringes from spores101... [Re: emrandel]
#7967095 - 02/01/08 11:04 PM (16 years, 28 minutes ago) |
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hey thanks for telling me those other companies that send their syringes already attached...maybe i am doing something else wrong like tahoe said but i cant think of anything other than i have never flame sterilized my needles(but i have succesfully grown b4, and im doing everything the same, but since ordering from spores101 i have been having probs) i guess i will try these syringes with this new found knowledge and if it doesn't work this time im gonna go with hawkseye. any other suggestions are welcome! thanks guys!
-------------------- git them mushrooms Mario! git em!
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Samurai_Jim
Samurai Emperor.


Registered: 01/12/08
Posts: 435
Loc: Near the Tail of the Drag...
Last seen: 1 year, 8 months
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Re: needles and syringes from spores101... [Re: The shroomy 1]
#7967676 - 02/02/08 05:37 AM (15 years, 11 months ago) |
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Quote:
The shroomy 1 said:
Did you thank them?;)
Should I have? After I ordered, I read some horror stories about stuff from them on here. I'll be lucky if my stuff actually grows. We'll see.
-------------------- Decreasing the world's bug population, one faceshield at a time.
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