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jccc
just aotherhuman



Registered: 12/01/06
Posts: 1,162
Last seen: 10 years, 11 months
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New WBS project
#7963937 - 02/01/08 08:53 AM (16 years, 15 hours ago) |
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I started a WBS project again i have done one before sussfully but i have problem already. I inoculated my 8 or so jars with an old lc that i recived in october and it has sat at room temp. until know(and in the dark) I inoculated he jars the morning of January 29th and this morning there is still no growth?! I know it seems like a short period to try and see results but it was an LC and im thinking it might have been dead from the get go.
So Should i find more LC culture and inoculate the same jars or give it more time? Thank YOu JCCC
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brainsOplenty
myconut



Registered: 06/27/06
Posts: 750
Loc: on your sister
Last seen: 1 year, 2 months
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Re: New WBS project [Re: jccc]
#7963948 - 02/01/08 08:57 AM (16 years, 15 hours ago) |
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Quote:
jccc said: I started a WBS project again i have done one before sussfully but i have problem already. I inoculated my 8 or so jars with an old lc that i recived in october and it has sat at room temp. until know(and in the dark) I inoculated he jars the morning of January 29th and this morning there is still no growth?! I know it seems like a short period to try and see results but it was an LC and im thinking it might have been dead from the get go.
So Should i find more LC culture and inoculate the same jars or give it more time? Thank YOu JCCC
don't rush it man. at least give it a couple of weeks, even three. before you give up on those jars. patience is the key, it's not like your making instant coffee.. LOL!!!
-------------------- FOR GOD'S SAKE MAN! TELL ME ABOUT THE FUCKING GOLF SHOES!!! LIVIN THE LIFE!!! "WE KNOCK NIGGAS OUT AND MAKE EM BOUNCE LIKE RICKY HATTON"- IMMORTAL TECHNIQUE
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jccc
just aotherhuman



Registered: 12/01/06
Posts: 1,162
Last seen: 10 years, 11 months
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Yeah I hear ya.. But this was inoculated with an LC so growth should begin within 24 hours of inoculations, and be visible withing 3 days.
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KillerPicklez



Registered: 12/13/07
Posts: 16,920
Loc:
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Re: New WBS project [Re: jccc]
#7964131 - 02/01/08 09:56 AM (16 years, 14 hours ago) |
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Quote:
jccc said: Yeah I hear ya.. But this was inoculated with an LC so growth should begin within 24 hours of inoculations, and be visible withing 3 days.
with an LC, especially an old one, id give it a week before you rule it out. you are right though, you should see something between 3-5 days.
dont most people keep LC's in a refrigerator, not at room temp?
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Neobean
Adept Mycologist


Registered: 10/07/01
Posts: 975
Loc: Canada
Last seen: 3 years, 8 months
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3 days is a short period, even for LC. Give it 5-6 days to see visible results, after that it will be lightning fast.
I think your lc should be ok for that amount of time at room temp, as long as it didn't get too hot...
-------------------- If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!
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jccc
just aotherhuman



Registered: 12/01/06
Posts: 1,162
Last seen: 10 years, 11 months
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Re: New WBS project [Re: Neobean]
#7964311 - 02/01/08 10:58 AM (16 years, 13 hours ago) |
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no it didnt get to hot cuase it was in the basement so thats a good thing...
I should have had them in the fridge but i forgot about them until i wanted to start a new project.. But ive heard somewhere on this site that if an lc is left to long at room temp. the mycellium goes into a sort of 'dormancy'...is this true? If so how do i snap them out of it? Do you want pics of the WBS?
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KillerPicklez



Registered: 12/13/07
Posts: 16,920
Loc:
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Re: New WBS project [Re: jccc]
#7964333 - 02/01/08 11:04 AM (16 years, 13 hours ago) |
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I believe it about the mycelium going into dormancy. although I have no proof, I always give my jars a cold shock before casing them. Its like my way of waking them up and telling them its time to go to work.
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jccc
just aotherhuman



Registered: 12/01/06
Posts: 1,162
Last seen: 10 years, 11 months
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Ok so what should i do if my jars so no growth in a week..give them some time in the fridge??
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KillerPicklez



Registered: 12/13/07
Posts: 16,920
Loc:
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Re: New WBS project [Re: jccc]
#7964715 - 02/01/08 12:33 PM (16 years, 11 hours ago) |
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Quote:
jccc said: Ok so what should i do if my jars so no growth in a week..give them some time in the fridge??
no, not at all. cold slows mycelium growth. I dont think putting uncolonized jars into the fridge would do any good at all. If you dont see anything by 1 week, maybe give it a couple more days, if nothing between 10-14, id probably toss them.
I only do the cold shock once my jars are 100% colonized.
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Neobean
Adept Mycologist


Registered: 10/07/01
Posts: 975
Loc: Canada
Last seen: 3 years, 8 months
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Even at 100%, there is no point in cold shocking cubensis. Only loses you 2-3 days.
-------------------- If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!
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KillerPicklez



Registered: 12/13/07
Posts: 16,920
Loc:
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Re: New WBS project [Re: Neobean]
#7964921 - 02/01/08 01:24 PM (16 years, 11 hours ago) |
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Quote:
Neobean said: Even at 100%, there is no point in cold shocking cubensis. Only loses you 2-3 days.
I dont just stick them in the fridge, I preform a cold dunk in the fridge which serves to both rehydrate my substrate after colonization and to cold shock the mycelium. Ive had very good results so im not too worried if I end up losing a couple days.
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Neobean
Adept Mycologist


Registered: 10/07/01
Posts: 975
Loc: Canada
Last seen: 3 years, 8 months
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Quote:
KillerPicklez said:
Quote:
Neobean said: Even at 100%, there is no point in cold shocking cubensis. Only loses you 2-3 days.
I dont just stick them in the fridge, I preform a cold dunk in the fridge which serves to both rehydrate my substrate after colonization and to cold shock the mycelium. Ive had very good results so im not too worried if I end up losing a couple days.
If you don't mind losing a few days, its A O.K. But I think you would achieve the same results from room temperature dunk under flowing water...
-------------------- If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!
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KillerPicklez



Registered: 12/13/07
Posts: 16,920
Loc:
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Re: New WBS project [Re: Neobean]
#7964986 - 02/01/08 01:42 PM (16 years, 10 hours ago) |
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Quote:
Neobean said:
Quote:
KillerPicklez said:
Quote:
Neobean said: Even at 100%, there is no point in cold shocking cubensis. Only loses you 2-3 days.
I dont just stick them in the fridge, I preform a cold dunk in the fridge which serves to both rehydrate my substrate after colonization and to cold shock the mycelium. Ive had very good results so im not too worried if I end up losing a couple days.
If you don't mind losing a few days, its A O.K. But I think you would achieve the same results from room temperature dunk under flowing water...
Probably, im one of those people that once I do something successfully several times, I dont like to change my methods. I like to change 1 variable of my grows at a time, to better gauge the benefits and consequences of the change.
Everyone just has their own way of doing stuff. I hate when people in mushroom cultivation get all uppity (not you at all, people in general) when people have their own methods and dont want to follow their cookie cutter outline for success.
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whereismymind
they keepcoming!



Registered: 04/12/04
Posts: 365
Loc: in the middle of nowhere
Last seen: 8 years, 6 months
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my new lc which was a grain lc , took 5 days to show signs first and then it was really fast , even had one that took 6 days to show anything. Peace
-------------------- In my own summer , there is no crowd in the streets and no sun...
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Neobean
Adept Mycologist


Registered: 10/07/01
Posts: 975
Loc: Canada
Last seen: 3 years, 8 months
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Quote:
KillerPicklez said:
Quote:
Neobean said:
Quote:
KillerPicklez said:
Quote:
Neobean said: Even at 100%, there is no point in cold shocking cubensis. Only loses you 2-3 days.
I dont just stick them in the fridge, I preform a cold dunk in the fridge which serves to both rehydrate my substrate after colonization and to cold shock the mycelium. Ive had very good results so im not too worried if I end up losing a couple days.
If you don't mind losing a few days, its A O.K. But I think you would achieve the same results from room temperature dunk under flowing water...
Probably, im one of those people that once I do something successfully several times, I dont like to change my methods. I like to change 1 variable of my grows at a time, to better gauge the benefits and consequences of the change.
Everyone just has their own way of doing stuff. I hate when people in mushroom cultivation get all uppity (not you at all, people in general) when people have their own methods and dont want to follow their cookie cutter outline for success.
No hard feelings, we are all in the same psychadelic boat 
-------------------- If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!
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KillerPicklez



Registered: 12/13/07
Posts: 16,920
Loc:
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Re: New WBS project [Re: Neobean]
#7965034 - 02/01/08 01:52 PM (16 years, 10 hours ago) |
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Quote:
Neobean said: No hard feelings, we are all in the same psychadelic boat 
of course not, we are all here to learn. I learn tons of new things everyday thanks to people like you.
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Day Tripper
Got a goodreason, for taking the easyway out.


Registered: 01/27/08
Posts: 198
Loc: Rocky Mountains
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Cold shocking has been around for ever, but was never needed with cubes, since they are tropical and never experience a temp drop in nature.
-------------------- Death is inevitable, and therefore irrelevant, life is optional, and therefore irreplaceable.
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jccc
just aotherhuman



Registered: 12/01/06
Posts: 1,162
Last seen: 10 years, 11 months
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Whereismymind: thats reassuring to hear that it took 6 days for signs of growth..because im so used to LC cultures growing quickly!
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whereismymind
they keepcoming!



Registered: 04/12/04
Posts: 365
Loc: in the middle of nowhere
Last seen: 8 years, 6 months
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Re: New WBS project [Re: jccc]
#7965628 - 02/01/08 04:32 PM (16 years, 7 hours ago) |
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well when they are new yes , but the one i told you about was also a grain lc which was sitting around for a long time , the mycelium was looking like bone dry , maybe that is why it took 6 days for it to recover . Anyway it can happen from time to time wait and see . Peace
-------------------- In my own summer , there is no crowd in the streets and no sun...
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jccc
just aotherhuman



Registered: 12/01/06
Posts: 1,162
Last seen: 10 years, 11 months
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No signs of growth now...Even my multi-spore innoculations are showing growth. So Im ganna order a new syringe.
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