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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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inoculated rye jars a little late
#7959628 - 01/31/08 10:04 AM (16 years, 1 day ago) |
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i didnt get around to innoculating my rye jars until 2 days after they had been pc'd. they just sat in the pc until then.
is this going to be a problem?
its been 3-4 days now since i inoculated them and i dont see any signs of life. i usually do by now. at least a little.
just wondering if i should re pressure cook them and start over?
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Neobean
Adept Mycologist


Registered: 10/07/01
Posts: 975
Loc: Canada
Last seen: 3 years, 8 months
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Re: inoculated rye jars a little late [Re: blood4blood]
#7959649 - 01/31/08 10:11 AM (16 years, 1 day ago) |
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Time is of essence in this art. ONe of the main reasons for PCing your jars is to give the mycelium a head start on contamination. By letting your jars sit there, you are voiding this advantage. I would toss and start over.
-------------------- If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!
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axis_tech
Rollin' Stoned


Registered: 02/27/05
Posts: 294
Last seen: 4 years, 9 months
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Re: inoculated rye jars a little late [Re: Neobean]
#7959657 - 01/31/08 10:17 AM (16 years, 1 day ago) |
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I would give them a few more day's and then after that give one of them the smell test. If you pressure cook them again it could throw off the water content.
-------------------- takes a token' and keeps on smoken'
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shroober
Myco Junkie

Registered: 01/02/08
Posts: 879
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Re: inoculated rye jars a little late [Re: Neobean]
#7959736 - 01/31/08 10:46 AM (16 years, 1 day ago) |
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Quote:
Neobean said: Time is of essence in this art. ONe of the main reasons for PCing your jars is to give the mycelium a head start on contamination. By letting your jars sit there, you are voiding this advantage. I would toss and start over.
Hmmm. If you PCed your jars and left them in an air tight PC no contams are going to be introduced. You've sterilized everything in there and assuming you didnt open the PC then theres no way for contams to get in. They should be fine in my opinion.
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Neobean
Adept Mycologist


Registered: 10/07/01
Posts: 975
Loc: Canada
Last seen: 3 years, 8 months
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Re: inoculated rye jars a little late [Re: shroober]
#7959751 - 01/31/08 10:49 AM (16 years, 1 day ago) |
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Quote:
shroober said:
Quote:
Neobean said: Time is of essence in this art. ONe of the main reasons for PCing your jars is to give the mycelium a head start on contamination. By letting your jars sit there, you are voiding this advantage. I would toss and start over.
Hmmm. If you PCed your jars and left them in an air tight PC no contams are going to be introduced. You've sterilized everything in there and assuming you didnt open the PC then theres no way for contams to get in. They should be fine in my opinion.
No contams from outside, but there are always remnants of contamination inside the jar, no matter how long you PC. Endospores, even after soaking and PC, produce more endospores. Why risk it anyways?
-------------------- If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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Re: inoculated rye jars a little late [Re: shroober]
#7959803 - 01/31/08 11:04 AM (16 years, 1 day ago) |
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Quote:
shroober said:
Quote:
Neobean said: Time is of essence in this art. ONe of the main reasons for PCing your jars is to give the mycelium a head start on contamination. By letting your jars sit there, you are voiding this advantage. I would toss and start over.
Hmmm. If you PCed your jars and left them in an air tight PC no contams are going to be introduced. You've sterilized everything in there and assuming you didnt open the PC then theres no way for contams to get in. They should be fine in my opinion.
this is what i was thinking... if contams get in, in the 2 days days i left them sit before innoculating whats stopping them from getting in in the 14 or so days it takes for them to colonize?
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xdaveman
Stranger

Registered: 02/28/07
Posts: 84
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Re: inoculated rye jars a little late [Re: blood4blood]
#7959809 - 01/31/08 11:07 AM (16 years, 1 day ago) |
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as long as you have some type of filter matierial you are fine. how do you think the sponsors can send jars of matierial?
Edited by xdaveman (01/31/08 11:13 AM)
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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Re: inoculated rye jars a little late [Re: xdaveman]
#7959850 - 01/31/08 11:18 AM (16 years, 1 day ago) |
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yea they have filter disks on them. ill just give them a little more time.
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SillyButtons
Naughty Santa



Registered: 03/04/07
Posts: 299
Loc: North Pole
Last seen: 7 years, 8 months
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Re: inoculated rye jars a little late [Re: shroober]
#7959890 - 01/31/08 11:36 AM (16 years, 1 day ago) |
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Quote:
shroober said: Hmmm. If you PCed your jars and left them in an air tight PC no contams are going to be introduced. You've sterilized everything in there and assuming you didnt open the PC then theres no way for contams to get in. They should be fine in my opinion.
    
I have let mine go almost a week before. No issues. Think about it, you can buy substrate jars and bags from sponsors here. The jars/bags are shipped to you. It's not like the sponsor comes to your kitchen and prepares stuff on site. If shipped bags weren't viable, you would hear about it all over the sponsors forum, "so and so ripped me off!"
-------------------- Travel on the cheap. Be a cultural ambassador. Spend the night on a stranger's couch! www.couchsurfing.com
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Day Tripper
Got a goodreason, for taking the easyway out.


Registered: 01/27/08
Posts: 198
Loc: Rocky Mountains
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Re: inoculated rye jars a little late [Re: SillyButtons]
#7960077 - 01/31/08 12:54 PM (16 years, 1 day ago) |
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You can wait a month or more, if your filter is good and your water content right.
I'm not saying it's good to wait, it's best to knock-up right out of the PC IMHO.
But I have seen rye jars sit for 6 weeks or more without issue, and while they began to ferment, they did not contam, nor pose a problem for aggressive myc to colonize.
Sounds crazy I know, but I say it with my own two eyes.
Try it sometime, put 6 rye jars aside for 2 months, then knock them up, remembering to shake them first.
-------------------- Death is inevitable, and therefore irrelevant, life is optional, and therefore irreplaceable.
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The shroomy 1
Luminous beings surround me




Registered: 03/27/07
Posts: 5,543
Loc: The Aether
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Re: inoculated rye jars a little late [Re: Day Tripper]
#7960134 - 01/31/08 01:13 PM (16 years, 1 day ago) |
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Quote:
Day Tripper said: You can wait a month or more, if your filter is good and your water content right.
I'm not saying it's good to wait, it's best to knock-up right out of the PC IMHO.
But I have seen rye jars sit for 6 weeks or more without issue, and while they began to ferment, they did not contam, nor pose a problem for aggressive myc to colonize.
Sounds crazy I know, but I say it with my own two eyes.
Try it sometime, put 6 rye jars aside for 2 months, then knock them up, remembering to shake them first.
Absolutely correct. My PC only holds 7 quart jars at a time, so by the time I get around to Pc'ing 24 jars and letting the last one's cool down overnight, at least 3 days have past by. With my work schedule I don't get around to inoculate them until about a week later,(If I'm lucky). Knowing this I PC them with lids that have no holes in them, thus they are held in a vacuum the entire time. I've held them for weeks at a time this way. I of course use a glove box when releasing the vacuum. If I use lids with inoculation holes in them, I have foil TIGHTLY wrapped on top of the lids. Weeks later, still no contams. Just be sterile in what you do and you'll be fine.
The Shroomy 1
--------------------
AMU Q&A thread.
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Day Tripper
Got a goodreason, for taking the easyway out.


Registered: 01/27/08
Posts: 198
Loc: Rocky Mountains
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Re: inoculated rye jars a little late [Re: The shroomy 1]
#7960197 - 01/31/08 01:32 PM (16 years, 1 day ago) |
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Good call on the glovebox Shroomy 1
-------------------- Death is inevitable, and therefore irrelevant, life is optional, and therefore irreplaceable.
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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Re: inoculated rye jars a little late [Re: Day Tripper]
#7960348 - 01/31/08 02:08 PM (16 years, 1 day ago) |
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radical. thanks guys. i figured it wouldn't be a problem but just needed the reassurance of my boys.
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Chapa888
Stranger

Registered: 01/31/08
Posts: 17
Last seen: 14 years, 15 days
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Re: inoculated rye jars a little late [Re: blood4blood]
#7960370 - 01/31/08 02:13 PM (16 years, 1 day ago) |
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i left jars in PC for over a week ....as long as you didn't open it more then a couple times for a second or two.....it was or is still sterile
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