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OfflineHappyCake
Stranger

Registered: 01/02/08
Posts: 18
Last seen: 16 years, 1 day
Newb's Failure
    #7959371 - 01/31/08 08:06 AM (16 years, 1 day ago)

Well, it is looking like i have failed my attempts to make a proper batch of jars for the second time.

I can't quite figure out what i have done wrong, but alas i continue on.

My first batch was RBF cakes with spore injection. Following PFtek pretty closely. Only one of those even took, and in that one, it was really weak. So i tossed em all.

I created a honey LC, mycelium grew, but it was thin and rested at the bottom. I was low in expectations.

I created a second batch, this time using Dark Rye flour, i heard good things. And a week later, i still have yet to see a single jar have any strong, if any mycelium growth.

No Contams, no growth.

I wonder if i just had bad spores. But i won't make that conclusion yet.

~HappyCake


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InvisibleMankey
Male

Registered: 07/30/05
Posts: 2,203
Re: Newb's Failure [Re: HappyCake]
    #7959550 - 01/31/08 09:32 AM (16 years, 1 day ago)

agar


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Offlinedjsethall
Dr. Justy
Male


Registered: 01/07/08
Posts: 88
Loc: PNW
Last seen: 2 years, 11 months
Re: Newb's Failure [Re: Mankey]
    #7959723 - 01/31/08 10:44 AM (16 years, 1 day ago)

Is the flour you are using "organic"?? Some grains have an antifungal additive that stops all fungi ffrom growing in it. I had the same problem a few years ago when I first tried a PF tek. I just used brown rice, not organic brown rice. End result? No growth whatsoever. I ended up finding that jar about 8 months later when I was moving and guess what, still no growth.


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Any posts made by me are purely fictional in nature and by no means is anything I say to be taken seriously. I do not grow or condone the growing of anything not legal. Any and all pictures I post are pictures wideley available on the internet and any discussions I am involved in are purely hypothetical or are commentary in nature and should not constitute advice or be considered advice to assist in activities that are deemed illegal.


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Offlineskattman1982
Stranger
Male

Registered: 09/18/07
Posts: 259
Last seen: 7 years, 11 months
Re: Newb's Failure [Re: HappyCake]
    #7959778 - 01/31/08 10:56 AM (16 years, 1 day ago)

Quote:

djsethall said:
Is the flour you are using "organic"?? Some grains have an antifungal additive that stops all fungi ffrom growing in it. I had the same problem a few years ago when I first tried a PF tek. I just used brown rice, not organic brown rice. End result? No growth whatsoever. I ended up finding that jar about 8 months later when I was moving and guess what, still no growth.




i buy bags of whole grain BR and grind it myself... saves from any additives that my cause bad things.

are you using a PC? believe me it makes a difference between success and failure. as far as having no signs of growth in a week... well some strains take longer to germinate and colonize especially if your tempurature is on the low side of 70. then again you may have gotten bad spores. i hope you used a sponsor.

with the LC you need to shake it every day or so once growth starts to break up the mycelium and allow it to colonize deeper into the solution.


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OfflineHappyCake
Stranger

Registered: 01/02/08
Posts: 18
Last seen: 16 years, 1 day
Re: Newb's Failure [Re: skattman1982]
    #7959861 - 01/31/08 11:23 AM (16 years, 1 day ago)

Both rye and RBF were bought and stamped "organic"
I bought medium coarse vermiculite

Yes, i used a Pressure cooker, i PCed at 15 psi for 40 minutes just to be extra cautious.

I am using B+ strain, and after inoculation i incubate at 80 degrees f, The spores i used to make the first batch and the LC were from a sponsored supplier.

I never thought about shaking the LC everyday, i will start doing that, hopefully it will help. I store it in the fridge as it has been well over two weeks incubating.

These are the reasons i go to the spores, but i really don't have enough evidence to really support this theory, as they did grow in my LC.

Overall, i think i did everything in my power to give the best growing conditions, alas, i just cant seem to get it right, yet.

A friend of mine said he had some spores that were viable, he is doing i guess the poo and straw tek (forgive me please.), then casing them. his flushes have been going strong, it is a pretty nice set up.

I guess one possible thing to look at is my water content. I keep going over and over in my head. A learning experience this is.

I'm going to let this batch incubate for another week before i toss them. I was hoping to have at least 1 flush done by spring break, but It doesn't look like i am going to make that, i'll wait.


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