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Offlinepolishfarmer
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contamination in FC
    #7958205 - 01/30/08 10:17 PM (16 years, 2 days ago)

i had a perfect 11/11 pf jar success and when i put them into the the fruiting chamber i lost 6 jars to contamination. Does anyone know why i got owned in the FC process? i thought it was smooth sailing after incubation. i had 2 tubs the 1st tub got completely contaminated and the second tub 5 out of 6 jars started pinning i took the 5th one out because it wasn't pinning and i saw a tiny spec of green on it.

can i do anything with the contaminated cakes ? someone said i can crush them and mix them with soil and put a plant in the soil and i should get some growth later from that. has anyone ever done th is before ??


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Invisiblethedefone
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Re: contamination in FC [Re: polishfarmer]
    #7958214 - 01/30/08 10:20 PM (16 years, 2 days ago)

What was the contaminate? Was it green? Are you sure it wasn't bruising?


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Offlinepolishfarmer
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Re: contamination in FC [Re: thedefone]
    #7958255 - 01/30/08 10:27 PM (16 years, 2 days ago)

in the first tub it was small green spots some were more developed than others and it looked like cobweb mold too.... it was like a fuzzy grayish mold that stuck out.... there were no signs of growth on any of those cakes. i just found one of the cakes in the second tub to have no growth and like 2 or 3 really small spots of green... i took it out because all the other cakes have started pinning and this one didnt


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Offlinepolishfarmer
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Re: contamination in FC [Re: polishfarmer]
    #7958475 - 01/30/08 11:12 PM (16 years, 2 days ago)

does anyone know anything about crushing up contam cakes and mixing with plant soil ?


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OfflineScavengerType
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Re: contamination in FC [Re: polishfarmer]
    #7958540 - 01/30/08 11:29 PM (16 years, 2 days ago)

how was fae and what was the method of humidification in the fc?


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"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
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Offlinepolishfarmer
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Re: contamination in FC [Re: ScavengerType]
    #7958844 - 01/31/08 12:47 AM (16 years, 2 days ago)

i used about 2 inches of perlte... i tried to fan air about 3 times a day... there were a few days that they went for half a day or so b4 i had a chance to fan fresh air in though


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OfflineScavengerType
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Re: contamination in FC [Re: polishfarmer]
    #7958961 - 01/31/08 01:49 AM (16 years, 2 days ago)

What was the water level in the perlite? BTW I've never got into fanning for FAE because you'd need to fan it more than that. Your definitely not getting a good FAE, but I don't know if this is the cause of your contaminants.


--------------------
"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club


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Offlineveda_sticks
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Re: contamination in FC [Re: ScavengerType]
    #7959277 - 01/31/08 06:42 AM (16 years, 1 day ago)

Tiny spec of green??? sure it wasnt bruising. If you suspect mold take a cotten bud etc and try and wipe it off. If it wipes of it is mould.

As for the cobweb are you absolulty sure it was cobweb and not just really thin mycelium growth reaching out in the humid air.

If they are definatly contamined bury then in the garden.

There are only 2 reasons i can think of contamination in the FC.

Not enough FAE.
Cakes were contaminated on the inside and you hadnt seen it yet.


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Invisibleblood4blood
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Re: contamination in FC [Re: veda_sticks]
    #7959291 - 01/31/08 06:53 AM (16 years, 1 day ago)

if it happened when you put it into fruiting that means that the mold spores have been there since inoculation and they stayed dormant throughout colonization and when you put them into fruiting conditions they sporulated.

check your sterility techniques for inoculating your jars. you messed up somewhere there.

there is nothing you can do with a contam'd cake but maybe bury it outside.id chuck it.


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OfflineRogerRabbitM
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Re: contamination in FC [Re: polishfarmer]
    #7959577 - 01/31/08 09:40 AM (16 years, 1 day ago)

Quote:

polishfarmer said:
i had a perfect 11/11 pf jar success and when i put them into the the fruiting chamber i lost 6 jars to contamination.




Contamination prior to first flush indicates your spawn was contaminated. Mold mycelium is white, thus you didn't notice it until sporulation.
RR


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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Offlinepolishfarmer
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Re: contamination in FC [Re: RogerRabbit]
    #7960135 - 01/31/08 01:14 PM (16 years, 1 day ago)

i was pretty sterile i heated and alcohol wiped the syringe b4 every jar.i did store them in the fridge for about 1 month to put them into hibernation.


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InvisibleDay Tripper
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Registered: 01/27/08
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Re: contamination in FC [Re: polishfarmer]
    #7960227 - 01/31/08 01:39 PM (16 years, 1 day ago)

RR is right, your sub was likely contamed at birth, or you efficiently fanned trich spores all over.

Maybe the room was contamed, that your FC is in, and when you fanned that first day trich spores landed everywhere.

One of those two happened IMHO.


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Death is inevitable, and therefore irrelevant, life is optional, and therefore irreplaceable.


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OfflineScavengerType
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Re: contamination in FC [Re: Day Tripper]
    #7961024 - 01/31/08 04:48 PM (16 years, 1 day ago)

I'd recomend judging from what people are saying that (if you don't already) you have a glove box. You never mentoned it so I thought you might be lacking on your sterile inoc procedures. Remember that's when it's most important. Additionaly it may be your method of air exchange. I'd recommend using one where the inflowing air is filtered, because of the possibility of having trich in your grow environment. Converting your FC into a Poor Man's Pod (see the fruiting chambers section) might solve this. Likely, since you say this was your first grow, your almost out of spores so Here's a guide on doing Liquid cultures I found useful. http://www.shroomery.org/forums/showflat.php/Number/5447083#5447083


--------------------
"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club


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InvisibleDay Tripper
Got a goodreason, for taking the easyway out.


Registered: 01/27/08
Posts: 198
Loc: Rocky Mountains
Re: contamination in FC [Re: ScavengerType]
    #7961032 - 01/31/08 04:50 PM (16 years, 1 day ago)

Clean that room out floor to ceiling, and make a glove box, and you should get to 99% in no time.


--------------------
Death is inevitable, and therefore irrelevant, life is optional, and therefore irreplaceable.


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Offlinepolishfarmer
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Re: contamination in FC [Re: Day Tripper]
    #7961559 - 01/31/08 06:50 PM (16 years, 1 day ago)

i didn't use a pressure cooker i just used a big giant pot with a lid and boiled about 2-3 inches of water for 2 hrs.... could that have had anything to do with it ??


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OfflineScavengerType
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Re: contamination in FC [Re: polishfarmer]
    #7962689 - 01/31/08 11:02 PM (16 years, 1 day ago)

no although I don't think you have to boil for two hours RR recommends 90 min but says many only boil for 1 hour. Did you remember to wipe everything in your glove box down with alcohol before you begin the inoc procedures?


--------------------
"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club


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OfflineSkeeblix
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Re: contamination in FC [Re: ScavengerType]
    #7962721 - 01/31/08 11:09 PM (16 years, 1 day ago)

If you're using perlite for humidity, there should be NO standing water at all. Perlite relies on its surface area to humidify, so any of it that's underwater may as well not even be there. It doesn't absorb water and release it like vermiculite does, it just has a very large surface area because it's covered in lots of tiny ridges and crevasses.

All you need to do is thoroughly moisten perlite before you put it in the FC, standing water just cuts the ability to humidify down, and it's a magnet for contamination.


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This post approved by:


Premedman1 said:
:lol: I just shat my pants.


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OfflineMuShQuEsT
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Re: contamination in FC [Re: Skeeblix]
    #7962971 - 02/01/08 12:15 AM (16 years, 1 day ago)

Someone above me suggested filtering your air. Don't take that to mean stuffing your holes with polyfil or the like. It may seem like that would help but your really shooting yourself in the foot. FAE combats contamination.

Also, no ones asked if your cakes were 100 percent when you birthed them? If they were you shouldn't have to worry much about contams getting a foothold on them


--------------------
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Fry: But existing is basically all I do!
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Offlinepolishfarmer
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Re: contamination in FC [Re: MuShQuEsT]
    #7964630 - 02/01/08 12:14 PM (16 years, 14 hours ago)

yeah im pretty sure they were 100%... i messed up on inoculating some of them and didnt see the solution run down the side of the jar. i didnt use a glove box and i did only moisten the perlite


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