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Invisibletahoe
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How to Tell if Your Lc is Contaminated
    #7956300 - 01/30/08 04:14 PM (16 years, 2 days ago)

here is a phot of a contamed lc compared to a clean one. Of course the clean one has a shit load of mycelium in it. The one that is contaminated was inoculated 3 days ago so there is not much mycelium growth.
I know what happened. I used a new scalpel and the chunk of mycelium that i was trying to drop into the jar would not fall off the blade. I had to hit the scalpel on the side of the jar to get the mycelium to fall off. By doing this I knocked loose something bad that was stuck on the scalpel up higher that was not killed by the alcohol bath.

BTW, the one on the right is bad. it is hazy,



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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


Edited by tahoe (01/30/08 05:14 PM)


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OfflineSamurai_Jim
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Re: How to Tell if Your Lc is Contaminated [Re: tahoe]
    #7956600 - 01/30/08 05:03 PM (16 years, 2 days ago)

Is the yellow one bad? Or the one on the right?

What LC process did you use?


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Decreasing the world's bug population, one faceshield at a time.


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Offlinetight
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Re: How to Tell if Your Lc is Contaminated [Re: Samurai_Jim]
    #7956619 - 01/30/08 05:06 PM (16 years, 2 days ago)

Quote:

Samurai_Jim said:
Is the yellow one bad? Or the one on the right?

What LC process did you use?




The one on the left is the clean one and it sounds like he cut a piece of a mushroom off under a flowhood and dropped it in the jar. Don't quote me on it though ^_^


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OfflineSkeeblix
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Re: How to Tell if Your Lc is Contaminated [Re: tight]
    #7956640 - 01/30/08 05:10 PM (16 years, 2 days ago)

No way, dude. Can't you see the chunks of dark colored shit floating around in the yellow one? Anything dark is bad. The right one is clean.


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Premedman1 said:
:lol: I just shat my pants.


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Invisibletahoe
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Re: How to Tell if Your Lc is Contaminated [Re: Skeeblix]
    #7956658 - 01/30/08 05:15 PM (16 years, 2 days ago)

the flash fucks up the color a bit.
The one on the right is bad. The left is clean and full of mycelium


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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OfflineSkeeblix
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Re: How to Tell if Your Lc is Contaminated [Re: tahoe]
    #7956669 - 01/30/08 05:16 PM (16 years, 2 days ago)

Yeah, okay, I see it now. It's not dark spots, it's just areas without mycelium. :lol:


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Premedman1 said:
:lol: I just shat my pants.


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Invisibletahoe
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Re: How to Tell if Your Lc is Contaminated [Re: Skeeblix]
    #7956678 - 01/30/08 05:18 PM (16 years, 2 days ago)

if you can read backwards you can see that it is blue oyster lc. I will use this in the pf tech woodlover post


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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OfflineHybridprX
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Re: How to Tell if Your Lc is Contaminated [Re: tahoe]
    #7957006 - 01/30/08 06:09 PM (16 years, 2 days ago)

Man, I don't mean to be attacking you Tahoe but that LC looks really poor....

and the yellow one, yea, obviously if your liquid culture turns a different shade then before you innoc it is bad but there is no way to tell if a LC is good or not until you inoculate a tester jar.

Listen guys, when it comes to a liquid culture ALWAYS innoc a tester jar before doing your final jar innoc's


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OfflineSamurai_Jim
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Re: How to Tell if Your Lc is Contaminated [Re: HybridprX]
    #7957032 - 01/30/08 06:13 PM (16 years, 2 days ago)

Quote:

HybridprX said:
Man, I don't mean to be attacking you Tahoe but that LC looks really poor....

and the yellow one, yea, obviously if your liquid culture turns a different shade then before you innoc it is bad but there is no way to tell if a LC is good or not until you inoculate a tester jar.

Listen guys, when it comes to a liquid culture ALWAYS innoc a tester jar before doing your final jar innoc's




Appriciate the advice, nice 11th post.


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Decreasing the world's bug population, one faceshield at a time.


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Invisibletahoe
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Re: How to Tell if Your Lc is Contaminated [Re: HybridprX]
    #7957035 - 01/30/08 06:14 PM (16 years, 2 days ago)

what?? Say again in english. Maybe use a few periods.

As for not looking good. Please show me one that looks better.
I did also mention that the color came out bad because of the flash


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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OfflineSamurai_Jim
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Re: How to Tell if Your Lc is Contaminated [Re: tahoe]
    #7957069 - 01/30/08 06:18 PM (16 years, 2 days ago)

Color? I have made LC's that looked like straight piss and they were fine. The longer you cook a sugar based substance, the more likely it will turn yellow. Malt extact gives a yellow LC, so does corn syrup.

But I have had nearly clear LC's with honey, I didn't PC it as long though. I have had them go both ways, turned out fine.


--------------------
Decreasing the world's bug population, one faceshield at a time.


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Invisibletahoe
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Re: How to Tell if Your Lc is Contaminated [Re: Samurai_Jim]
    #7957119 - 01/30/08 06:25 PM (16 years, 2 days ago)

well mojo if my lc's were hazey from the get go then how would I know if they were contamed before use???
Clearity means a lot


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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InvisibleMojo
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Re: How to Tell if Your Lc is Contaminated [Re: tahoe]
    #7957137 - 01/30/08 06:29 PM (16 years, 2 days ago)

I deleted that post because Samurai_Jim beat me to it. It wasn’t directed toward you anyway.

However, I said nothing about haziness so I don’t even know what you’re referring to.

An LC can be dark and still be clear.


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OfflineSamurai_Jim
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Re: How to Tell if Your Lc is Contaminated [Re: tahoe]
    #7957140 - 01/30/08 06:29 PM (16 years, 2 days ago)

Quote:

tahoe said:
well mojo if my lc's were hazey from the get go then how would I know if they were contamed before use???
Clearity means a lot




+1 No disagreement here. Have you ever done a LC with the Potatoe water / dextrose? In my thread over there, you can see the LC after 5 days. It had potatoe particulate in the water. But I have done others that were clear as a bell.


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Decreasing the world's bug population, one faceshield at a time.


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Offlineemrandel
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Re: How to Tell if Your Lc is Contaminated [Re: Samurai_Jim]
    #7957291 - 01/30/08 07:06 PM (16 years, 2 days ago)

Thanks for the comparison, I appreciate that as I have a couple LC's going and am very paranoid about the contam. I had three batches that were likely to have been perfectly fine but threw them away out of paranoia.

We'll know soon enough.


--------------------
"No drug, not even alcohol, causes the fundamental ills of society. If we're looking for the source of our troubles, we shouldn't test people for drugs, we should test them for stupidity, ignorance, green and love of power!" - P.J. O'Rourke


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Invisibleblood4blood
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Registered: 04/25/07
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Re: How to Tell if Your Lc is Contaminated [Re: emrandel]
    #7957500 - 01/30/08 07:54 PM (16 years, 2 days ago)







not to hijack your thread tahoe. but this was my attempt at cloning by dropping a couple core samples into an lc medium which was karo based (probably to much karo. 4% I'm pretty sure)

how does this look? does it look like mycelium or just the cores deteriorating to you guys?

the solid looking parts in there are the cores but they are getting all whispy and stringy around them. oh and i dropped the cores in there on the 15th


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Invisibletahoe
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Re: How to Tell if Your Lc is Contaminated [Re: blood4blood]
    #7957520 - 01/30/08 07:57 PM (16 years, 2 days ago)

that looks good. The growth look correct and the lc didnt turn cloudy


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Invisibleblood4blood
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Registered: 04/25/07
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Re: How to Tell if Your Lc is Contaminated [Re: tahoe]
    #7957572 - 01/30/08 08:04 PM (16 years, 2 days ago)

its not cloudy no, but there are little white dots floating everywhere that i cant pick up with my camera is that still alright?

do you think its done growing and shold be put in the fidge?

it didnt really do anything for the first week or so but the past few days ive noticed the whispyness around the cores and some white stuff floating around. its really tuff for me to tell. ive never had any turn out like yours that are so pronunced. but i got that dry malt i sent you a pic of that im going to try 2morrow with that lc tek of yours.


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Invisibletahoe
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Re: How to Tell if Your Lc is Contaminated [Re: blood4blood]
    #7958290 - 01/30/08 10:35 PM (16 years, 2 days ago)

it is good enough to use now but let it grow for another week, about twice that much mycelium before you fridge it.

My lc's get so that that when I use them a layer of mycelium lays on top of the grain after injection. The finer mycelium runs down into the grain with the liquid. If I do not shake there will be a visible white lump after a few hours. I will take a pic of this tomorrow if i remeber. I have 4 jars reay to be inoculated and I am tired and going skiing tomorrow


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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OfflineScavengerType
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Re: How to Tell if Your Lc is Contaminated [Re: tahoe]
    #7958609 - 01/30/08 11:46 PM (16 years, 2 days ago)

so using this criteria your LC should be good:

- The liquid be the same color be the same as after cooked.
- The liquid should not be cloudy.
- The mycillium should be making ropey formations in the liquid.

Anything I missed on assessing your LC?

As well if your culture looks like this is a test culture necessary? I want to inoculate right away so any time savers? Also while on the topic what thickness should mycillium be in an LC before it is used?


--------------------
"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
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