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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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LC'S and 4% Sugar content
#7955411 - 01/30/08 12:48 PM (16 years, 2 days ago) |
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I have always wondered where people came up with this 4% thing and while sitting in the office reading through ggmm I decided to read the liquid culture recipe again. I have read it before but never really got caught up in it. Stamets says to use 40 grams of malt with 1000ml of water. I use malt but i do not use anywhere near this amount. When i was experimenting I started with 20 grams of malt as per most agar recipes and 1000ml of water. This is 2%. I found that this is still too rich and discolored the water too much. So i dropped it down to 4 grams malt to 1000ml and then down to 2 grams malt to 1000ml and now I am at 1 gram per 1000ml of water.
Thats it 1 gram per 1000ml. What is that .1% sugar? This is all i use and my lc's look better than most of the pics I see here. I highly suggest that you guys cut back on the sugar. More is not always better. The lower sugar content will also help with contams. Give it a shot and use powdered malt. i bet you will never go back to honey,karo, turbino, dextrose, or whatever you are using
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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Neobean
Adept Mycologist


Registered: 10/07/01
Posts: 975
Loc: Canada
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Re: LC'S and 4% Sugar content [Re: tahoe]
#7955424 - 01/30/08 12:53 PM (16 years, 2 days ago) |
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Quote:
tahoe said: I have always wondered where people came up with this 4% thing and while sitting in the office reading through ggmm I decided to read the liquid culture recipe again. I have read it before but never really got caught up in it. Stamets says to use 40 grams of malt with 1000ml of water. I use malt but i do not use anywhere near this amount. When i was experimenting I started with 20 grams of malt as per most agar recipes and 1000ml of water. This is 2%. I found that this is still too rich and discolored the water too much. So i dropped it down to 4 grams malt to 1000ml and then down to 2 grams malt to 1000ml and now I am at 1 gram per 1000ml of water.
Thats it 1 gram per 1000ml. What is that .1% sugar? This is all i use and my lc's look better than most of the pics I see here. I highly suggest that you guys cut back on the sugar. More is not always better. The lower sugar content will also help with contams. Give it a shot and use powdered malt. i bet you will never go back to honey,karo, turbino, dextrose, or whatever you are using
Can you post pics of this .1% sugar LC? I am not doubting, just curious to the end result. If it really works as great as you say it does, I will definitely be giving this a try sooner rather than later
-------------------- If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!
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shroober
Myco Junkie

Registered: 01/02/08
Posts: 879
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Re: LC'S and 4% Sugar content [Re: tahoe]
#7955433 - 01/30/08 12:55 PM (16 years, 2 days ago) |
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Do you notice any difference in colonization times?
Where do you get powdered malt?
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: LC'S and 4% Sugar content [Re: Neobean]
#7955462 - 01/30/08 01:04 PM (16 years, 2 days ago) |
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Pics will be proof more than my words??? Only because your avatar is nice will I take the time to do so.
notice the overgrown lc. I am waiting for it to pin for you guys. The mycelium may look a little different than what you are used to. This are not ps strains. One is oyster, lions mane, sra, portabella. The last one in the cap is sra. it will be placed in a pray bottle and sprayed in suitable areas for future wild outdoor hunting. Note the last one is lighter in color. There is less mycelium in it and just appears clearer. it was inoculated 2 days ago
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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Neobean
Adept Mycologist


Registered: 10/07/01
Posts: 975
Loc: Canada
Last seen: 3 years, 8 months
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Re: LC'S and 4% Sugar content [Re: tahoe]
#7955561 - 01/30/08 01:25 PM (16 years, 2 days ago) |
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Quote:
tahoe said: Pics will be proof more than my words??? Only because your avatar is nice will I take the time to do so.
my avatar has unlimited power 
apart from the .1% sugar, what is your tek?
-------------------- If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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Re: LC'S and 4% Sugar content [Re: Neobean]
#7955573 - 01/30/08 01:27 PM (16 years, 2 days ago) |
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how many cc's of spore soulution do you recommend putting into 500ml's of lc medium?
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Neobean
Adept Mycologist


Registered: 10/07/01
Posts: 975
Loc: Canada
Last seen: 3 years, 8 months
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Re: LC'S and 4% Sugar content [Re: blood4blood]
#7955582 - 01/30/08 01:30 PM (16 years, 2 days ago) |
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Quote:
blood4blood said: how many cc's of spore soulution do you recommend putting into 500ml's of lc medium?
The beauty of this stuff is that you need less than 1cc of spore solution or left over lc to make another lc!
-------------------- If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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Re: LC'S and 4% Sugar content [Re: Neobean]
#7955595 - 01/30/08 01:33 PM (16 years, 2 days ago) |
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shut up! i was thinking more of 3 cc's but ill try 1. ill be busy tonight i just got a bag of dry malt in the mail yesterday and have been wanting to try this for awhile. i really need a good lc!
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: LC'S and 4% Sugar content [Re: blood4blood]
#7955596 - 01/30/08 01:33 PM (16 years, 2 days ago) |
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maybe 1/4-1/2cc but I would never recomend using a spore syringe for a lc.
But, if you do then dont over do it. No more than 1/2 cc should be used for anything requiing a spore syringe. You will ust be wasting it. If this was 5 gallons of lc you would only use 1/4-1/2cc. the size of the lc,grain,pf cake doesnt matter
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: LC'S and 4% Sugar content [Re: tahoe]
#7955611 - 01/30/08 01:36 PM (16 years, 2 days ago) |
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i use a 1/4 inch square chunk of mycelium for my lc's. I cut it up into a pile of mycelium on the petri dish. Then scoop it up and drop into the lc, this is done in a glove box with the lid on the lc only being open for a few seconds
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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Re: LC'S and 4% Sugar content [Re: tahoe]
#7955675 - 01/30/08 01:47 PM (16 years, 2 days ago) |
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i havent gotten into agar yet, so im stuck using a spore syringe.
is there any other way of doing it besides a spore syringe for me?
i know i could drop a core sample from a fruit in there and make a clone but i also dont have any fruits at the moment.
why do you not recommend using a syringe tahoe? just from them being so unpredictable?
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: LC'S and 4% Sugar content [Re: blood4blood]
#7955724 - 01/30/08 01:56 PM (16 years, 2 days ago) |
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they can be dirty. You can use one and it will work great if it is clean work fine. Problem is that you can not tell if a ss is clean unless you use it. If it is from a good supplier and you trust it than go for it. If the clearity of the lc/water changes at all just the slightest bit hazey than it is probably contamed. Contams that might not show up on grain using a ss will multiply 100X on a lc and then once you use it on grain/cake you are fucked.
Get what I am saying. lets say that there are 100 bad mold spores in a syringe. When used on grain the mycelium is able to beat the bad mold to the nutrients first and the mycelium fights off the mold. This is because the bad mold doesnt have a good chance of moving around on the grain once it is attached to a kernel or brf/verm.
In a lc this bad mold spore can float around and will be able to germinate and go to town in the lc along with the mycelium. if you do not know what you are looking for in a lc than you might not notice it. It isnt like a layer of green is going to be floating on it. So now you suck up this lc with thick chunks of mycelium and a slurry of bad shit. This bad shit will blow up in the grain now and you have a nasty mess.
Get what I am saying. This is another reason why you use so little of you ss in an lc.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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Re: LC'S and 4% Sugar content [Re: tahoe]
#7955775 - 01/30/08 02:05 PM (16 years, 2 days ago) |
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i know what your saying mang. its a very valid point brother.
i make my own syringes and i guess its the only way i have to make an lc right now since i wont be doing any agar in the near future.
ill make 3- 4 differnt ones and go from there. and once i know i have a good viable one ill just start using that for innoc'n future ones.
its either ss or a core sample for me at this point. but i really want some good lc. goddamnit!
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
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Re: LC'S and 4% Sugar content [Re: blood4blood]
#7955960 - 01/30/08 02:52 PM (16 years, 2 days ago) |
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ss might be better then core sample. I have made clean ss that I have used for lc's I say give it a shot. Just use the lc to inoculate 1 jar once the lc is colonized. If it stays clean then you will be good to go
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: LC'S and 4% Sugar content [Re: tahoe]
#7956271 - 01/30/08 04:08 PM (16 years, 2 days ago) |
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this is what a 1/2 gram looks like. Not much for a lc??? Its just right
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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castle212
Cannibal



Registered: 11/05/07
Posts: 36
Loc: The Twilight Zone
Last seen: 2 months, 14 days
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Re: LC'S and 4% Sugar content [Re: tahoe]
#7956643 - 01/30/08 05:12 PM (16 years, 2 days ago) |
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What kind of malt do you use? Light Dry Malt? So that is all you use in your LC's besides water? I may give this a go instead of MM's tek because his calls for tubinado, light corn syrup, dextrose, and light dry malt all at once. Your method just seems a lot easier.
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IF YOU DON'T LIKE MY FIRE THEN DON'T COME AROUND CAUSE I'M GONNA BURN ONE DOWN. - Ben Harper
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tahoe
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Registered: 11/26/03
Posts: 6,274
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Re: LC'S and 4% Sugar content [Re: castle212]
#7956668 - 01/30/08 05:16 PM (16 years, 2 days ago) |
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mm knows his shit but that sure is a lot of sugar. I just use light tan malt as per the ggmm
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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