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Invisibletahoe
Noob Slayer
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Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: contams...any ideas where we went wrong? [Re: stellabella11]
    #7953544 - 01/29/08 10:45 PM (16 years, 3 days ago)

Quote:

stellabella11 said:
we did the pf tek. watched the video. followed directions to a T...




if you followed directions to a t than it was a dirty syringe.
Now did you really follow to a t.
1/2 oint wide mouth?
4 holes in the lid?
wiped down the sides of the jars?
1/2verm layer
cook for 1 hour in a pot or pc'ed for 20 mins?
flamed the needle after every 4th jar?
did not suck up any brf into the syringe?
1/4cc per hole?
brf?

did water get into the jar during cooking?
did you tip the jar from side to side after inocaulation?

if it was truely followed to a t than it had to be a dirty syringe
Where did you get it? Spores101?


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Invisibletahoe
Noob Slayer
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Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
Trusted Cultivator
Re: contams...any ideas where we went wrong? [Re: tahoe]
    #7953710 - 01/29/08 11:09 PM (16 years, 3 days ago)

just look at this post. Same sort of deal. This person claims that everything was done correctly but I have my doubts

http://www.shroomery.org/forums/showflat.php/Cat/0/Number/7945426/an/0/page/1


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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OfflineSkeeblix
Dave Thomas
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Registered: 01/28/07
Posts: 1,745
Loc: Wendy's
Last seen: 8 years, 3 months
Re: contams...any ideas where we went wrong? [Re: tahoe]
    #7954011 - 01/30/08 12:07 AM (16 years, 3 days ago)

Well, there's no point being cynical to the point of elitism, man. You do it in a lot of your posts, and just because you've grown for however many tens of years doesn't mean you have to be so harsh to the people who haven't.

If someone wants to try harder to be sterile, giving them some pointers should be all it takes to get them to "clean up their act" (bad pun intended). Otherwise questioning their methods with the eye of a nagging stepmother is a bit much.

Smoke one and remember you were young and 'tarded once, too. :tongue:

To be a broken record for stellabella11, contams only at your inoculation points is a good sign of a contaminated syringe, provided you correctly sterilized your syringe between jars. Flame until your needle is red hot, and do it after every jar to remove any possibility of contamination between jars.


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This post approved by:


Premedman1 said:
:lol: I just shat my pants.


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Invisibletahoe
Noob Slayer
Male User Gallery


Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
Trusted Cultivator
Re: contams...any ideas where we went wrong? [Re: Skeeblix]
    #7954815 - 01/30/08 08:59 AM (16 years, 2 days ago)

if one truely follows teh pf tech/mmgg to the "T" and they fail it is the syringe. There is no need to be "extra sterile" with that tech. It was developed so people who follow diretions can have success with minimal effort. Those who fail surprise me. It isnt hard, not even close.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


Extras: Filter Print Post Top
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