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Offlinekronik08
Stranger

Registered: 01/09/08
Posts: 74
Last seen: 15 years, 11 months
attempt numbr two
    #7951608 - 01/29/08 05:42 PM (16 years, 3 days ago)

well i posted yesterday on the mildewish smell coming from the jars i had started about 5 or 6 days ago. i cam home today and the smell was still there with no sign of myc growth. so i decided to toss them out since im not really low on supplies for now and get this weekend, neways..
i used pre made myc jars that i had ordered that were pre sterilized, i figured i couldnt go wrong with the sterilization process if the jars were presterilized, this way i could concentrate more on the inoculation process so if nething goes wrong i know thats where the mistake was made. i cleaned my desk really well with clorox clean up then flame sterilized and wiped with alcohol between jars, i noced up 12 jars and put them in my incubation chamer, ive heard several times that this isnt required but like it cuz of the controlled environment, also i live in south tx and if a northern comes in, its coming and im with no heat in my room with equals about 50 to 60 degree temps, so thats y im using the incubation chamber.

did i take enough of a precaution in sterilizing my work area. i also cleaned my hands really well along with my face and put on clean clothes and noced them up. ps i sprayed the room with the clorox
thx for all replies.


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Offlinekronik08
Stranger

Registered: 01/09/08
Posts: 74
Last seen: 15 years, 11 months
Re: attempt numbr two [Re: kronik08]
    #7951686 - 01/29/08 06:02 PM (16 years, 3 days ago)

btw the jars had one hole in the center with a cotton swab as a filter and the bag simply said put the needle into the jar inject upto 2cc's in each jar so i only noced them up in only one spot right in the center. is this ok??


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Offlineasknoquestions
Stranger

Registered: 01/16/08
Posts: 97
Last seen: 14 years, 4 months
Re: attempt numbr two [Re: kronik08]
    #7951701 - 01/29/08 06:05 PM (16 years, 3 days ago)

You really should use at least latex gloves and a facemask. There is no way your hands are getting sterile. If you continue to have sterilization issues, you might consider building a glovebox.


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Offlinekronik08
Stranger

Registered: 01/09/08
Posts: 74
Last seen: 15 years, 11 months
Re: attempt numbr two [Re: asknoquestions]
    #7951933 - 01/29/08 06:43 PM (16 years, 3 days ago)

thx that was actually what i was thinking. if this attempt turns out the same way im gonna build a glove box and take further precautions. what im wondering is will innoculating them in the middle only, give me results. should i shake the jars to move the substrate around and spread the spores


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OfflineSeventy
equanimitor
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Registered: 05/24/07
Posts: 912
Loc: Canada (PNW)
Last seen: 6 months, 28 days
Re: attempt numbr two [Re: kronik08]
    #7952263 - 01/29/08 07:45 PM (16 years, 3 days ago)

if you're poor like me you can attempt a budget glovebox like the one in my sig

it works. I made it myself.


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