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OfflineSubbedhunter420
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Re: Possible So cal active (pluteus?) [Re: cactu]
    #8322744 - 04/24/08 11:16 AM (15 years, 9 months ago)

Im just glad they finally identified it. Or so we think...Hahaw

Well Im gonna go tell my friend whom I found them with that theyre inocybes. Im always gonna be looking for these again. I have a feeling I can find many more in the season to come.


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OfflineWorkmanV
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Re: Possible So cal active (pluteus?) [Re: Subbedhunter420]
    #8322796 - 04/24/08 11:28 AM (15 years, 9 months ago)

I am glad too. It encourages me to do the microscopy. I might have even figured out the genus if I had already done it since I know what Inocybe cystidia look like.

Excellent conclusion to this mystery.


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InvisiblegeorgeM
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Re: Possible So cal active (pluteus?) [Re: Alan Rockefeller]
    #8322980 - 04/24/08 12:25 PM (15 years, 9 months ago)

You are persistent when it comes to seeking out mushroom IDs Alan! Did Brandon examine multiple dried fruitbodies or just a sample for microscopy?

Cool mycoweb page, though I prefer Subbedhunter's photo...

Subbed, you might consider inquiring as to whither they would like to add a copy of your photo to the page.


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InvisibleCureCat
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Re: Possible So cal active (pluteus?) [Re: Alan Rockefeller]
    #8324704 - 04/24/08 08:55 PM (15 years, 9 months ago)

Excellent work!!

I think Brandon's got this one- thanks for the persistence Alan!  I think you totally tracked down the answer.  Awesome.  An active Inocybe.... that's rad.

I've been doing so many extractions lately, I am quite confident that Else wouldn't mind me throwing this one in with one of the batches.  If you want, next time I see you i can collect a few gill fragments to work on at the lab, eventually to be sequenced.  :ohwell:


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OfflineAlan RockefellerM
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Re: Possible So cal active (pluteus?) [Re: canid]
    #8329445 - 04/26/08 03:10 AM (15 years, 9 months ago)

> Psilocybian compound[s] and muscarine, that's a horrid combination.


Its not a combination, its one or the other. No psilocybin mushrooms have been found that have other toxins.

Brandon saw this thread and would like to clarify that "European I. corydalina does NOT have muscarine but does have psilocybin. Kuyper (1986) summarizes literature up to that point and reports that others report "all hallucinogenic Inocybe-species were dovoid of muscarine" (Persoonia 3(Suppl.):12).

Inocybe calamistrata belongs to a group that also does not have muscarine, but these are not hallucinogenic."

> I think Brandon's got this one

Yea me too.


> I've been doing so many extractions lately, I am quite confident that Else wouldn't mind me throwing this one in with one of the batches. If you want, next time I see you i can collect a few gill fragments to work on at the lab, eventually to be sequenced.


How much gills do you need for your DNA extraction?


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InvisibleCureCat
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Re: Possible So cal active (pluteus?) [Re: Alan Rockefeller]
    #8331986 - 04/26/08 08:02 PM (15 years, 9 months ago)

>How much gills do you need for your DNA extraction?

Approximately the size of 4-6 individual bee wings. But a bit more might be good if you can spare, just in case. Also, if you do it, be careful about it... I usually flame sterilize a razor blade and cut off a few small slivers of the lamelle, then put inside a sterile eppendorf with sterile glass beads for the extraction. You gotta be careful not to accidentally get any other fungal DNA in the sample. Less worried about plant matter or whatever, as we use fungi-specific primers.


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OfflineAlan RockefellerM
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Re: Possible So cal active (pluteus?) [Re: CureCat]
    #8333336 - 04/27/08 05:35 AM (15 years, 9 months ago)

> Approximately the size of 4-6 individual bee wings.

So some scientists are using the bee wing scale for DNA extraction these days?

I guess thats cool but if I get stung while trying to measure you out some gills for this sample I will be kind of pissed.

Also honey bees are getting really hard to find these days, perhaps you should suggest that they switch to the jellow jacket scale.


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InvisibleCureCat
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Re: Possible So cal active (pluteus?) [Re: Alan Rockefeller]
    #8333340 - 04/27/08 05:41 AM (15 years, 9 months ago)

I don't know... I'm sort of allergic to yellow jackets.


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OfflineAlan RockefellerM
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Re: Possible So cal active (pluteus?) [Re: Workman]
    #9779179 - 02/11/09 04:08 PM (14 years, 11 months ago)

Quote:

Workman said:Excellent conclusion to this mystery.




It gets better...

Brandon Matheny said:

"Inocybe aff. corydalina. Should be described as a new potentially hallucinogenic species of Inocybe.

This species is closely related to Inocybe corydalina from Europe, but material collected by Kevin Bock in the Santa Monica Mountains on 28-Jan-2008 under Quercus is only 91% similar to European corydalina when comparing their ITS sequences. Off the top of my head, I can’t think of any greening or blue-greening species of Inocybe described from North America with metuloid cystidia and smooth spores.

Regards, Brandon Matheny
University of Tennessee"


"Only 91% similar" is quite a bit of difference from another species in the same genus that looks the same.  Looks like subbedhunter420 has quite a bit of writing to do, maybe Brandon can help a bit.


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OfflineSubbedhunter420
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Re: Possible So cal active (pluteus?) [Re: Alan Rockefeller]
    #9782631 - 02/12/09 01:15 AM (14 years, 11 months ago)

Ok... well it looks like I need more help.

Good note though! Just by chance, (Or by season and fortunate wet weather!) the suspected Inocybes have popped up in the same spot exactly. About 2 weeks difference of a year. They were found last week and previously I found them on January 28th 2008.

They blue a lot more than the previous bunch too! I will post pictures soon.


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