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Invisiblepsiloglow
Stranger
Registered: 07/07/07
Posts: 115
Bulk Substrate, 1st timer. Bag style, Questions, help!
    #7947888 - 01/28/08 09:27 PM (16 years, 4 days ago)

Ok, heres what I wanted to do.

I was thinking of using the usual mixture of PF tek substrate, but in mass amounts to be stuffed into Autoclave bags. Autoclave them, cool, and inoculate with Spore syringes. I was reading about liquid cultures being used, but thought a syringe or 2 per bag should be enough to slowly innoculte the whole thing, just as the jars would, but in one huge cake basically? Am I correct in my assumptions? Eventually I wanted to move chunks of this to sterilized cow dung/verm mix and inoculate that into patties and so forth. If this is no good please advise on easiest method to do bulk using bags and pressure cooker. Does substrate have to be seeds, or grains?, and does liquid culture HAVE to be used. thanks for the support.


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Invisiblethedefone
deus ex machina

Registered: 10/06/07
Posts: 1,883
Loc: Gondwana
Re: Bulk Substrate, 1st timer. Bag style, Questions, help! [Re: psiloglow]
    #7947995 - 01/28/08 09:46 PM (16 years, 4 days ago)

Quote:

Does substrate have to be seeds, or grains?, and does liquid culture HAVE to be used.



LC doesn't have to be used, but it does speed things up.

Half-pints of BRF take ~2-3 weeks on average with multispore inoculation. BRF pints take around 10 days longer. A spawn bag with a half gallon of BRF will finish colonizing long after you are dead. If you want to step up to larger amounts of spawn, you need to find where they sell rye berries in your town, and buy them.

Quote:

a syringe or 2 per bag should be enough to slowly innoculte the whole thing



You can easily inoculate 3 gallons of rye with 2 10cc syringes, and they will totally finish in ~2 weeks. Compare that to your BRF spawn bag, and I hope you'll see whats most economical, rational, and reasonable. Good Luck.


--------------------


I am become death, the destroyer of worlds.


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