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OfflineKing_of_Dank
hustla baby
Male

Registered: 02/09/07
Posts: 46
Loc: Amsterdam
Last seen: 14 years, 9 days
Lighting and Sterilizing
    #7945576 - 01/28/08 02:22 PM (16 years, 4 days ago)

I'm a beginner trying out the PF-Tek method obviously and the guide says to use a drip shield, but then how would the cakes get sunlight? Should I make sure the plexi glass is totally transparent? Also I would like to know the best way to sterilize my terrarium. I read people talking about a glove box...WTF is that?


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OfflineMYSTIQUE
Say Hi to the elves for me.
 User Gallery


Folding@home Statistics
Registered: 04/28/07
Posts: 1,764
Loc: Canada
Last seen: 5 years, 4 months
Re: Lighting and Sterilizing [Re: King_of_Dank]
    #7945745 - 01/28/08 03:03 PM (16 years, 4 days ago)

drip sheild is soooooooooooooo 1900's get in the new.


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Dont know what the fuck I just said? READ THIS
http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary
I ain't a hippy but I'm covered in dirt                         
Sippin lots of mushroom tea in a tye-dye shirt
Chasin' the Grateful Dead, no shoes on my feet
Beggin' in the parking lot for something to eat,

:onfire:DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!:onfire:


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Invisibleshroober
Myco Junkie


Registered: 01/02/08
Posts: 879
Re: Lighting and Sterilizing [Re: MYSTIQUE]
    #7945751 - 01/28/08 03:05 PM (16 years, 4 days ago)

search for RR shotgun tek

best way to go for beginners


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Invisiblethedefone
deus ex machina

Registered: 10/06/07
Posts: 1,883
Loc: Gondwana
Re: Lighting and Sterilizing [Re: shroober]
    #7945775 - 01/28/08 03:10 PM (16 years, 4 days ago)

Quote:

Also I would like to know the best way to sterilize my terrarium. I read people talking about a glove box...WTF is that?



Spray alcohol. Wipe down. Spray white vinegar. Wipe down. Sterilized.

A glove box is a also called a still-air box. Seventy had one that he made on the super-cheap in his signature, but that is for inoculations, and grain-to-grain transfers, and agar work, and stuff where contamination is a big concern. Fully colonized cakes will tend not to contaminate right off the bat. Good luck.


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I am become death, the destroyer of worlds.


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Invisiblebadman
Male User Gallery

Registered: 06/14/06
Posts: 4,039
Re: Lighting and Sterilizing [Re: King_of_Dank]
    #7945809 - 01/28/08 03:19 PM (16 years, 4 days ago)

what i do to sterilize the FC is to wash it out with washing up liquid rinse with water, make it bone dry. Then use a 10% v/v soultion of bleach and water, rinse again and make it bone dry. To dry i use a microfiber cloth really absorbent. Lastly clean with isoproyl (95-100%)and let dry.

The lid should allow light though ive used both transparent and frosted before both good but dont put them in direct sunlight. As for a drip shield its a good idea but not a must have item.

A glove box is a sealed box with two hole cut out of it, in these holes are gloves, again making the box sealed. This allows you to work in sealed off environment free of contaminants. Clean the glove box like above. (Do a google image search for glove box if you unsure)


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