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InvisibleBrain Fart
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Registered: 12/19/07
Posts: 2,538
Loc: Your Mom Flag
A lesson for beginners...
    #7945426 - 01/28/08 01:47 PM (16 years, 4 months ago)

Steralize everything..take all necessary precautions..it is not a pain in the ass, it is a necessity.


All 9 of my jars have contamed just today due to skipping sterilization procedures. (No glovebox, didn't wipe surfaces)

It is a shitty feeling and it pisses you off to see all the prep work goto waste.

Aghhh back to sqaure one, anyone wanna lend a print for one in the future?


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Offlinedeadatdusk
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Re: A lesson for beginners... [Re: Brain Fart]
    #7945485 - 01/28/08 02:01 PM (16 years, 4 months ago)

I've had successful grows without a glove box, but having one is really important. My lungs would hurt for days after bleach bombing my house in an attempt to inoculate without a glove box.

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InvisibleCaptain Cubensis
Bleeding HeartLiberal


Registered: 09/18/07
Posts: 648
Re: A lesson for beginners... [Re: deadatdusk]
    #7945504 - 01/28/08 02:03 PM (16 years, 4 months ago)

Use a glovebox, no matter what you hear. Sterilize for 2 hours to be certain, and pasteurize and casing materials for 1-2 hours.


--------------------
www.groworganic.com
Get 350lbs shipped to your door for only $100!
www.mycosupply.com
Online Organic Rye Berries
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The Cyclestat 4P, a great repeat cycle timer made in the USA.

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InvisibleBrain Fart
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Re: A lesson for beginners... [Re: Captain Cubensis]
    #7945738 - 01/28/08 03:00 PM (16 years, 4 months ago)

Steralize everything, over steralization does not exist. The more sterile the better.

Hoenstly, you will not regret taking the extra time to do it.


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Invisibletahoe
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Registered: 11/26/03
Posts: 6,274
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Re: A lesson for beginners... [Re: Brain Fart]
    #7945750 - 01/28/08 03:05 PM (16 years, 4 months ago)

Quote:

Brain Fart said:
Steralize everything..take all necessary precautions..it is not a pain in the ass, it is a necessity.


All 9 of my jars have contamed just today due to skipping sterilization procedures. (No glovebox, didn't wipe surfaces)





were you doing agar transfers?


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread

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Offlineveda_sticks
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Re: A lesson for beginners... [Re: Brain Fart]
    #7945887 - 01/28/08 03:46 PM (16 years, 4 months ago)

I have been saying this for months, use a glovebox. yes some people have success in open air but why take the risk when a simple cheap glovebox works well and effectivly.

Since i started using a glovebox my success rate has been 100%. Even in my untidy and not so clean flat i have great success with a glovebox, before i had around a 90% failure.

The best thing you can do is be clean and sanatiry with your work areas, don't go around bleach bombing everyone. For a start its very bad to breath bleach fumes and its impossible to get rid of it all. Keeping spore counts low is all thats nessesary. Which can be obtained with regular cleaning and sanitising of surfaces, and not allowing foods to lie around.


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PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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InvisibleCaptain Cubensis
Bleeding HeartLiberal


Registered: 09/18/07
Posts: 648
Re: A lesson for beginners... [Re: veda_sticks]
    #7945901 - 01/28/08 03:49 PM (16 years, 4 months ago)

Right on bro, 100% success with a glove box, they rule! And for $10...forget about it.

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Offlineveda_sticks
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Re: A lesson for beginners... [Re: Captain Cubensis]
    #7946009 - 01/28/08 04:13 PM (16 years, 4 months ago)

My glove box cost me nothing, i had a clear plastic box lyng around, honestly dont know where it came from, cut 2 holes in it and away i was went for 3/4 jars contaming to 0/4 contaming (only 4 jars at a time?? small pressure cooker and i dont need that much shrooms)

Even a cardboard box can work as a glovebox.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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InvisibleBrain Fart
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Registered: 12/19/07
Posts: 2,538
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Re: A lesson for beginners... [Re: tahoe]
    #7948240 - 01/28/08 10:41 PM (16 years, 4 months ago)

Quote:

tahoe said:
Quote:

Brain Fart said:
Steralize everything..take all necessary precautions..it is not a pain in the ass, it is a necessity.


All 9 of my jars have contamed just today due to skipping sterilization procedures. (No glovebox, didn't wipe surfaces)





were you doing agar transfers?





Nope just reg pf tek jars.


I saw a really good tek for a cheap ass glovebox that works very well in someones link. But forgot the name and to lazy to look.

The avatar is the guy wearing the hood. Said it costed him like 2$ and works like a charm.

Blehh...Use a glovebox. I'm speaking from the heart newb-to-newb here.


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Invisibletahoe
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Re: A lesson for beginners... [Re: Brain Fart]
    #7949334 - 01/29/08 07:18 AM (16 years, 4 months ago)

pf tek jars done correctly do not require a glove box. They do not even remotely require a glove box. I am 99% sure that you fucked up somewhere else. Show us a jar and the contam pattern.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread

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Offlinewhereismymind
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Re: A lesson for beginners... [Re: tahoe]
    #7949350 - 01/29/08 07:25 AM (16 years, 4 months ago)

clear plastic box + gloves for washing dishes = glovebox actually too easy . Maybe i havent done a lot of times g2g but till now i ve never had a contamination even through g2g , inoculation of jars is something people can get away with in open air , if they dont have a bad syringe or if they dont clean around the place that they are working they may get contamination of course .
Peace .


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In my own summer , there is no crowd in the streets and no sun...

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OfflineKickleM
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Re: A lesson for beginners... [Re: whereismymind]
    #7949890 - 01/29/08 10:38 AM (16 years, 4 months ago)

I have never used a glove box when inoculating PF jars, and the only contamination I've had was when boiling a jar and it contacted the bottom of the pan cracking it.


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Why shouldn't the truth be stranger than fiction?
Fiction, after all, has to make sense. -- Mark Twain

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OfflineFahkface
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Re: A lesson for beginners... [Re: Kickle]
    #7950070 - 01/29/08 11:28 AM (16 years, 4 months ago)

It's known that you don't necessarily need a glove box for PF jars or any syringe inoculation process. It helps, yes, but the main reason for your failure was probably the syringe making process and free floating mold spores.

You shouldn't have any bigger problems with contams when your home is not mold infected.
My house is quiet dirty, but there seems to be few mold spores etc. and therefore I work without ANY contamination for a long time now.
I sterilize my jars for 45 mins. in the PC make the syringes and inoculation process in the kitchen without any Lysol / bleach etc. and keep getting good results without any contams.
IF you have a mold problem (which you seem to have), a glove box is indeed a good decision, but when everything is normal, it's not necessary!

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Offlinegaladar


Registered: 05/09/07
Posts: 981
Loc: in a monotub!
Last seen: 2 years, 7 months
Re: A lesson for beginners... [Re: Fahkface]
    #7950208 - 01/29/08 12:12 PM (16 years, 4 months ago)

dont be lazy noobs.

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OfflineHybridprX
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Re: A lesson for beginners... [Re: galadar]
    #7950698 - 01/29/08 02:48 PM (16 years, 4 months ago)

Building a well rounded glove box is key to this hobby.

Smitty's head friends tell Smitty that if doing tissue / mycelium / spores to direct substrate, a glove box or Lamar flow hood are essential to success in this hobby.

Smitty has had much higher success rates since using either G2G or spores to direct substrate innoc.

Look for Smitty's split personalities sure shot no failure guide coming up.

:wink:


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InvisibleBEEP
Registered: 01/06/08
Posts: 1,385
Re: A lesson for beginners... [Re: Brain Fart]
    #7951294 - 01/29/08 04:46 PM (16 years, 4 months ago)

Quote:

Brain Fart said:
Sterilize everything, over sterilization does not exist. The more sterile the better.

Honestly, you will not regret taking the extra time to do it.




my first inoculation i used about half a bottle of rubbing alcohol and wrapped my arms in saran wrap+gloves i cleaned my glove box with alcohol i cleaned my lighter with it even my syringes i even gave my jars a quick rub before i took the tape off the holes i washed my arms with the alcohol and Lysol too. nothing should live through that :P..

i thought i was over sterilizing, but it will probably reward me.

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OfflineThe shroomy 1
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Re: A lesson for beginners... [Re: Brain Fart]
    #7954071 - 01/30/08 12:20 AM (16 years, 4 months ago)

Quote:

Brain Fart said:
Quote:

tahoe said:
Quote:

Brain Fart said:
Steralize everything..take all necessary precautions..it is not a pain in the ass, it is a necessity.


All 9 of my jars have contamed just today due to skipping sterilization procedures. (No glovebox, didn't wipe surfaces)





were you doing agar transfers?





Nope just reg pf tek jars.


I saw a really good tek for a cheap ass glovebox that works very well in someones link. But forgot the name and to lazy to look.

The avatar is the guy wearing the hood. Said it costed him like 2$ and works like a charm.

Blehh...Use a glovebox. I'm speaking from the heart newb-to-newb here.



That would be xaxphaanes


--------------------


AMU Q&A thread.

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OfflineSeventy
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Registered: 05/24/07
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Re: A lesson for beginners... [Re: veda_sticks]
    #7954187 - 01/30/08 12:57 AM (16 years, 4 months ago)

Quote:

veda_sticks said:
Even a cardboard box can work as a glovebox.




he tells the truth, I made one myself

I even have it right in my sig

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OfflineSupplier
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Re: A lesson for beginners... [Re: Seventy]
    #7954301 - 01/30/08 01:40 AM (16 years, 4 months ago)

look this cost me like $11.00 at lowes.. the only thing wrong is i got one with a red lid non see threw haha so i just need a clear lid or make a plexyglass spot to look in.



--------------------
:mushroomgrow: :pinkshroom: :mushroomgrow: :pinkshroom: :mushroomgrow:

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InvisibleBEEP
Registered: 01/06/08
Posts: 1,385
Re: A lesson for beginners... [Re: Supplier]
    #7954402 - 01/30/08 02:45 AM (16 years, 4 months ago)

Quote:

Supplier said:
look this cost me like $11.00 at lowes.. the only thing wrong is i got one with a red lid non see threw haha so i just need a clear lid or make a plexyglass spot to look in.






even easier, you could tape some saran wrap oer the top as a lid or just flip it over and use the lid as the bottom, but i see you already cut the holes where you want your arms.

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