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InvisibleDay Tripper
Got a goodreason, for taking the easyway out.


Registered: 01/27/08
Posts: 198
Loc: Rocky Mountains
Simple Clone Tek
    #7942092 - 01/27/08 07:07 PM (16 years, 5 days ago)

It doesn't get any easier than this guys.

Try dropping a small, but promising pin, maybe from a clump, or branching off a large fruit, into a fresh grain jar.

You will be surprised how well it can work if you are clean and quick.

After the jar is 110% colonized, use it for G2G.


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Death is inevitable, and therefore irrelevant, life is optional, and therefore irreplaceable.


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Invisibledumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
Re: Simple Clone Tek [Re: Day Tripper]
    #7942119 - 01/27/08 07:14 PM (16 years, 5 days ago)

WOW...I HAD NO IDEA!!! !!! !!!


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Offlineresptodd
I reject yourreality andsubstitute myown
Male User Gallery


Registered: 10/16/07
Posts: 674
Loc: Michigan
Last seen: 1 year, 10 months
Re: Simple Clone Tek [Re: dumbfounded1600]
    #7942153 - 01/27/08 07:23 PM (16 years, 5 days ago)

Does the pin want to continue to grow?


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Damn! I'm having fun! Just keep the GD monkeys away.


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InvisibleDay Tripper
Got a goodreason, for taking the easyway out.


Registered: 01/27/08
Posts: 198
Loc: Rocky Mountains
Re: Simple Clone Tek [Re: resptodd]
    #7942169 - 01/27/08 07:25 PM (16 years, 5 days ago)

No, it will abort and dry up, but the myc at the base of the pin branches out quickly.

Try it man, it is an amazingly easy way to clone.


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Death is inevitable, and therefore irrelevant, life is optional, and therefore irreplaceable.


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OfflineSamurai_Jim
Samurai Emperor.
Male

Registered: 01/12/08
Posts: 435
Loc: Near the Tail of the Drag...
Last seen: 1 year, 8 months
Re: Simple Clone Tek [Re: Day Tripper]
    #7942357 - 01/27/08 08:01 PM (16 years, 5 days ago)

Well, cloning really should be done from the mature fruit. That way you know exactly what you are cloning. If you clone the biggest mature fruit, the genetic disposition is there for the clones to be just as big. Cloning a pin would keep you from knowing exactly what the fruit would look like when mature.

Otherwise, it's a good idea. Maybe transfer to agar?


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Decreasing the world's bug population, one faceshield at a time.


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InvisibleDay Tripper
Got a goodreason, for taking the easyway out.


Registered: 01/27/08
Posts: 198
Loc: Rocky Mountains
Re: Simple Clone Tek [Re: Samurai_Jim]
    #7942374 - 01/27/08 08:05 PM (16 years, 5 days ago)

True, true, but if I see a big clump of pins, it's safe to say that those genes are good enough for me brother man!

Like when several grow from one pin, well that's sweet for this tek, cause you could take our 5-10 pins, and drop them into quarts of rye, and have a great genetic line.

Maybe it's not quite an isolation on agar, but it's much easier, which is the point here.

But yes, you are right, maybe take the bottom off the largest fruit?

Little pins seem like less contam risk, but why not just cut a little tissue sample from a large fruit and drop it right into a jar.

Skips the LC step altogether.


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Death is inevitable, and therefore irrelevant, life is optional, and therefore irreplaceable.


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Offlineresptodd
I reject yourreality andsubstitute myown
Male User Gallery


Registered: 10/16/07
Posts: 674
Loc: Michigan
Last seen: 1 year, 10 months
Re: Simple Clone Tek [Re: Samurai_Jim]
    #7942382 - 01/27/08 08:09 PM (16 years, 5 days ago)

That was gonna be my next question. How can you be sure the pin is going to be a quality fruit, something that you would want to clone? Which raises another question for me,- What would you look for in a shroom if you are wanting to clone?


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Damn! I'm having fun! Just keep the GD monkeys away.


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OfflineSamurai_Jim
Samurai Emperor.
Male

Registered: 01/12/08
Posts: 435
Loc: Near the Tail of the Drag...
Last seen: 1 year, 8 months
Re: Simple Clone Tek [Re: Day Tripper]
    #7942386 - 01/27/08 08:10 PM (16 years, 5 days ago)

Well, it definately would work. Espically taking from the healthiest patch of pins.

Agar isolation isn't really hard. It is actually easy as pie. Keeping sterile work environments is dificult though. Through agar iso, you can get THE best rhizo activity. Isolate several times and then transfer to grain or LC.

You are right on about being quick using a pin from fruiting myc. It would be a good way to get a lot of jars rolling quick.


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Decreasing the world's bug population, one faceshield at a time.


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InvisibleDay Tripper
Got a goodreason, for taking the easyway out.


Registered: 01/27/08
Posts: 198
Loc: Rocky Mountains
Re: Simple Clone Tek [Re: Samurai_Jim]
    #7942391 - 01/27/08 08:12 PM (16 years, 5 days ago)

Thanks bro!

"What would you look for in a shroom if you are wanting to clone?"

Size, potency, maybe aesthetic, whatever qualities you like, you clone.


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OfflineSamurai_Jim
Samurai Emperor.
Male

Registered: 01/12/08
Posts: 435
Loc: Near the Tail of the Drag...
Last seen: 1 year, 8 months
Re: Simple Clone Tek [Re: resptodd]
    #7942394 - 01/27/08 08:13 PM (16 years, 5 days ago)

Quote:

resptodd said:
That was gonna be my next question. How can you be sure the pin is going to be a quality fruit, something that you would want to clone? Which raises another question for me,- What would you look for in a shroom if you are wanting to clone?




You want to look for a good healthy fruit. I would say that differant people would want to clone differant types of specimens. Personal preference to a degree.


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Decreasing the world's bug population, one faceshield at a time.


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InvisibleDay Tripper
Got a goodreason, for taking the easyway out.


Registered: 01/27/08
Posts: 198
Loc: Rocky Mountains
Re: Simple Clone Tek [Re: Samurai_Jim]
    #7942443 - 01/27/08 08:22 PM (16 years, 5 days ago)

True man, true, but sometimes people forget about cloning from clumps because they only clone from large fruits.

If you clone from a pin in a large clump, you will get thicker pin sets.

If that pin happens to have good size genes, you get thick pin sets with large fruits.


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Offlinegrod31
Stranger
Male


Registered: 12/06/07
Posts: 2,077
Loc: New York
Last seen: 14 years, 11 months
Re: Simple Clone Tek [Re: Day Tripper]
    #7942872 - 01/27/08 09:36 PM (16 years, 5 days ago)

so im getting a pf albino syringe. from my understanding since this is a multispore its not guaranteed that any or all of the shrooms that grow will be albino. With this tek i can take the first albino pin(s) and drop them into grain jars to have a 100% chance those jars will produce albino. a verry quick way to get started, 5 shrooms for you man. and for everyone else, once this works there will be clear prints going out.


--------------------
Back the tape up.  I need it again!
Let it roll!  Just as high as the
fucker can go!  And when it comes
to that fantastic note where the
rabbit bites its own head off, I
want you to  THROW THAT FUCKING
RADIO INTO THE TUB WITH ME!
    Not me.  It would blast you through
the wall stone dead in ten
seconds and they'd make me explain
VVVVV Free Myco Thread


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Offlinegrod31
Stranger
Male


Registered: 12/06/07
Posts: 2,077
Loc: New York
Last seen: 14 years, 11 months
Re: Simple Clone Tek [Re: grod31]
    #7942885 - 01/27/08 09:38 PM (16 years, 5 days ago)

it says banned- i guess no shrooms for you. but there will still be prints.


--------------------
Back the tape up.  I need it again!
Let it roll!  Just as high as the
fucker can go!  And when it comes
to that fantastic note where the
rabbit bites its own head off, I
want you to  THROW THAT FUCKING
RADIO INTO THE TUB WITH ME!
    Not me.  It would blast you through
the wall stone dead in ten
seconds and they'd make me explain
VVVVV Free Myco Thread


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OfflineDragonChaser
Ice in Her Ass and Pussy
Male User Gallery

Registered: 04/27/06
Posts: 7,212
Last seen: 6 years, 2 months
Re: Simple Clone Tek [Re: grod31]
    #7942958 - 01/27/08 09:48 PM (16 years, 5 days ago)

Lol, maybe he got banned because he had 50 posts, but an average of 375 per day. Posted too much too fast I guess.


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My name is Mud


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Invisiblesmokedout420
The EnlightenedOne
Male User Gallery


Registered: 12/16/07
Posts: 484
Re: Simple Clone Tek [Re: DragonChaser]
    #7944404 - 01/28/08 09:28 AM (16 years, 4 days ago)



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Invisibledumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
Re: Simple Clone Tek [Re: smokedout420]
    #7944566 - 01/28/08 10:13 AM (16 years, 4 days ago)

Meaty doesn't mean anything its just water based


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