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OfflineLothario
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Why did LC stall pics?
    #7935843 - 01/26/08 03:45 PM (16 years, 6 days ago)

This lc is several weeks old. It is 300ml of distilled water with 1 full tablespoon of Karo. I had it on a magnetic stir plate at about 78-80F was shot up with spore solution. It is completely contam free but won't fully colonize. I've used it to inoculate 10 jars of rye with 100% success. My question is what should I do differently next time to end up with a fully colonized jar of lc? What did I do wrong?







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Invisibletahoe
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Re: Why did LC stall pics? [Re: Lothario]
    #7935952 - 01/26/08 04:08 PM (16 years, 6 days ago)

because it is air tight?????
I do not see any way for fresh air to get in.

Do differntly next time? How about .5-1 gram of malt powder mixed with 500ml of water. How about a polyfill or syringe filter for some fresh air


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Invisibletahoe
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Re: Why did LC stall pics? [Re: tahoe]
    #7935957 - 01/26/08 04:09 PM (16 years, 6 days ago)

as for growth if that thing is clean than it is fine. That lump of mycelium is more than enough for the lc. Blend it and suck it up. IF they get over grown than its hard to suck into a syringe


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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OfflineLothario
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Re: Why did LC stall pics? [Re: tahoe]
    #7935965 - 01/26/08 04:11 PM (16 years, 6 days ago)

There is a 1/4in hole covered with micropore tape. It's hard to see in the pic. This was my first attempt and I can't figure it out.


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OfflineLothario
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Re: Why did LC stall pics? [Re: Lothario]
    #7935977 - 01/26/08 04:12 PM (16 years, 6 days ago)

I thought the whole thing should colonize?


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Invisibletahoe
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Re: Why did LC stall pics? [Re: Lothario]
    #7935979 - 01/26/08 04:12 PM (16 years, 6 days ago)

are you sure its not contamed? you used a lot of karo, maybe it was too much ad the mycelium is retarded.
I would just use powdered light malt next time.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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OfflineLothario
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Re: Why did LC stall pics? [Re: tahoe]
    #7936036 - 01/26/08 04:24 PM (16 years, 6 days ago)

I thought my ratio was right on.

1 teaspoon per 100ml

3 teaspoons = 1 tablespoon

I used 1 tablespoon for 300ml.

Thanks though I'll def try the malt.


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Invisibletahoe
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Re: Why did LC stall pics? [Re: Lothario]
    #7936115 - 01/26/08 04:42 PM (16 years, 6 days ago)

it might be right on but karo sucks. This might be one reason why.
malt and potato broth are key for lc's but the potato broth is too hazy so i prefer malt


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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OfflineLothario
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Re: Why did LC stall pics? [Re: tahoe]
    #7936188 - 01/26/08 05:05 PM (16 years, 6 days ago)

Ah well I'll check it out. Thanks for your help, I know I can find some at the brew store around here. I just have one more question. At this point should I refrigerate or should I keep it warmer temps in the hopes it'll grow a little more?


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Invisibletahoe
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Re: Why did LC stall pics? [Re: Lothario]
    #7936254 - 01/26/08 05:25 PM (16 years, 6 days ago)

depends on why it isnt growing. If it is still good then I would put it in the fridge


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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OfflineLothario
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Re: Why did LC stall pics? [Re: tahoe]
    #7937726 - 01/26/08 09:58 PM (16 years, 6 days ago)

That is the question at hand, why isn't it growing? I think I'll just stick it in the fridge and use what I can. If only I could get the stir bar outta there :laugh:


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Invisiblesmokedout420
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Re: Why did LC stall pics? [Re: Lothario]
    #7937754 - 01/26/08 10:03 PM (16 years, 6 days ago)

you could use a airport syringe to help with fresh air...


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