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Invisibledumbfounded1600
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Registered: 07/29/07
Posts: 2,624
Snake Venom Into Hybrids
    #7924741 - 01/24/08 03:29 PM (16 years, 4 months ago)

Does anyone know anything about this...would be quiet nice to do something of that sort...gonna go rattle snake hunting this summer and hopefully bringing back a bunch of snakes

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OfflineRogerRabbitM
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Re: Snake Venom Into Hybrids [Re: dumbfounded1600]
    #7925309 - 01/24/08 05:30 PM (16 years, 4 months ago)

Search this forum for snake venom and hybridization.
RR


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OfflineFunkatron9000
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Re: Snake Venom Into Hybrids [Re: RogerRabbit]
    #7925710 - 01/24/08 06:43 PM (16 years, 4 months ago)

What on earth would that do!? You trying to kill someone?


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OfflineRogerRabbitM
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Re: Snake Venom Into Hybrids [Re: Funkatron9000]
    #7925732 - 01/24/08 06:48 PM (16 years, 4 months ago)

Yes, mycelium cell walls. It helps the nuclei to do whatever it is they do.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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OfflineFunkatron9000
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Re: Snake Venom Into Hybrids [Re: RogerRabbit]
    #7925780 - 01/24/08 06:57 PM (16 years, 4 months ago)

Thats just to crazy! lol, How did someone figure that out ya know...
"Hmmm I think I'll toss some snake venom into this petri here and um... oh crap hybridized myc."

I'm sure there was much more thought in it.... but still.


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You cannot yet touch my daughters awesome boobage
:jiggly:
You must fight to the death in the Breastriary of Nippopolis.




I thought Gene Wilder was cool BEFORE he was dead.

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Invisibledumbfounded1600
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Re: Snake Venom Into Hybrids [Re: Funkatron9000]
    #7927139 - 01/24/08 10:14 PM (16 years, 4 months ago)

It breaks down the cell walls in order for them to form

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Invisibletahoe
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Re: Snake Venom Into Hybrids [Re: dumbfounded1600]
    #7927535 - 01/24/08 11:25 PM (16 years, 4 months ago)

uhhhh


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


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InvisibleJohn NadaDiscord
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Re: Snake Venom Into Hybrids [Re: tahoe]
    #7928563 - 01/25/08 05:38 AM (16 years, 4 months ago)

For real???? Goddamn!

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Invisibledumbfounded1600
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Re: Snake Venom Into Hybrids [Re: John Nada]
    #7929101 - 01/25/08 09:56 AM (16 years, 4 months ago)

pshhh ..... yeah

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Offlinemr_minds_eye
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Re: Snake Venom Into Hybrids [Re: dumbfounded1600]
    #7934024 - 01/26/08 08:52 AM (16 years, 4 months ago)

I noticed in the original post that there was refrence to other things being used for this purpose. No one ever mentioned what they were. What are the other additives that could promote that?


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Our quest for discovery fuels our creativity in all fields, not just science. If we reached the end of the line, the human spirit would shrivel and die. But I don't think we will ever stand still: we shall increase in complexity, if not in depth, and shall always be the center on an expanding horizon of possibilities.
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OfflineRogerRabbitM
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Re: Snake Venom Into Hybrids [Re: mr_minds_eye]
    #7934584 - 01/26/08 11:41 AM (16 years, 4 months ago)

I first learned of rattlesnake venom from attending a medicinal mushroom conference in 2005. John Holliday did a presentation on it. Here's a link to a paper he wrote. John Holliday is director of research at alahomedicinals.com and there's lots more info on his website.
RR
http://www.nwbotanicals.org/nwb/lexicon/hybridcordyceps.htm


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Invisibledumbfounded1600
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Re: Snake Venom Into Hybrids [Re: RogerRabbit]
    #7934642 - 01/26/08 11:59 AM (16 years, 4 months ago)

I got a friend who has a ranch and has SHITLOADS of rattles every year...it gets up in the 100s...Im gonna start going up there and milking it..but I dont know how to keep it viable I mean...will it lose its shit being in a air tight container? Can I keep it in the freezer?

Im thinking of doing it and sending it to mycologist for free...I love snakes and amphibians...im also trying to raise Bufo Alvarius Toads and try breeding in captivity without the injection of hormones

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Invisiblefastfred
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Re: Snake Venom Into Hybrids [Re: dumbfounded1600]
    #7935739 - 01/26/08 03:27 PM (16 years, 4 months ago)

> breeding in captivity without the injection of hormones

Good luck with that. Keep us posted on that in ODD and C&P. I raised one for quite awhile.

I know someone with a bunch of poisonous snakes, so I wonder what kinds of venom would be useful?

I also wonder if the activity is due solely to weakening the cell walls. Chitinase would then work for this also. PEG helps for fusing protoplasts so maybe that would also be a useful additive.


-FF

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OfflineRogerRabbitM
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Re: Snake Venom Into Hybrids [Re: fastfred]
    #7935770 - 01/26/08 03:32 PM (16 years, 4 months ago)

The venom is not heat stable, so it needs to be added just before pouring the agar, after it's cooled almost to the point of solidifying. For this reason, it must be completely sterile. Sigma Scientific sells purified snake venom, freeze dried into a powder. It has to be stored at -20C or less until use.

I'm not sure of their process, but I've never had it contaminate the agar. It's about three times the price of powder cocaine, so not for everyone who just wants to experiment.

Search this forum for the Redboy thread(s). I had some 20 year old redboy spores that simply would not germinate, no matter what I tried. After placing the non-viable spores on venomated agar for a couple of weeks, I released monokaryotic PR mycelium to crawl over the dish. Low and behold, when the monokaryotic mycelium reached the area where the spores were near the edge, two separate rhizomorphic strains emerged. Somehow the snake venom allowed the PR mycelium to access the nucleus of the spores for mating. I had tried this without the venom to no avail. The very first time venom was used, the result was dikaryotic mycelium, and the redboy strain was back as a 50/50 cross with PR. The experiment was repeated with the offspring, resulting in 75% Redboy(perhaps? I'm not a geneticist), which was then released to the community.
RR

Edited by RogerRabbit (01/26/08 03:42 PM)

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Invisibledumbfounded1600
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Re: Snake Venom Into Hybrids [Re: RogerRabbit]
    #7936055 - 01/26/08 04:26 PM (16 years, 4 months ago)

Can I get the name to that site?

Interesting story...is the venom expensive?

This process sucks now lol(milking and selling the venom)...I'd have to get a freeze drier because I know now freezer that I have goes below -20...I don't think

Edited by dumbfounded1600 (01/26/08 04:27 PM)

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InvisibleDontPlay
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Re: Snake Venom Into Hybrids [Re: dumbfounded1600]
    #7939245 - 01/27/08 07:14 AM (16 years, 4 months ago)

Not to mention you shouldn't be running around picking up rattlesnakes and grabbing their head to milk them. Your not the crocodile hunter lol. Feel free to post after your hospital experience.


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InvisibleKnoa6
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Re: Snake Venom Into Hybrids [Re: DontPlay]
    #7939316 - 01/27/08 08:22 AM (16 years, 4 months ago)

this is very interesting!
RR, have you ever successfully hybridized two strains?

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Offlinewikedferret
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Re: Snake Venom Into Hybrids [Re: Knoa6]
    #7939429 - 01/27/08 09:22 AM (16 years, 4 months ago)

he just said he did with pr and red boy


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lol i just lied to whoever was reading that.....

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Offlinewikedferret
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Re: Snake Venom Into Hybrids [Re: wikedferret]
    #7939456 - 01/27/08 09:33 AM (16 years, 4 months ago)

oh and RR, on that note if you didnt use all of your red boy spores why dont you repeat it with your PR and RB hybrid and make it 75% RB and 25% PR and again until their are only trace amounts or PR to get that RB back? by the 5th try it would be at a 6.25% out of 100 chance that it would resemble a PR that leaves 93.75% RB


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lol i just lied to whoever was reading that.....

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OfflineRogerRabbitM
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Re: Snake Venom Into Hybrids [Re: wikedferret]
    #7939626 - 01/27/08 10:31 AM (16 years, 4 months ago)

Quote:

oh and RR, on that note if you didnt use all of your red boy spores why dont you repeat it with your PR and RB hybrid and make it 75% RB and 25% PR




Isn't that exactly what I said I did in my last post? :shrug:

The 75% RB/PR cross used up the last of the original 20 year old spores.  It's all covered in the Redboy thread from four years ago.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds High THC Strains   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags   Myyco.com Golden Teacher Liquid Culture For Sale   OlympusMyco.com Olympus Myco Bulk Substrate   North Spore Injection Grain Bag   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Kraken Kratom Red Vein Kratom


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