Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

Magic-Mushrooms-Shop.com
This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Advanced Mycology
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds High THC Strains   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   Myyco.com Golden Teacher Liquid Culture For Sale   Kraken Kratom Red Vein Kratom   Mushroom-Hut Substrate Bags   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Sporeworks.EU Spores for European Microscopy   PhytoExtractum Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: 1 | 2 | 3 | 4 | 5 |  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Snake Venom Into Hybrids
    #7924741 -

Does anyone know anything about this...would be quiet nice to do something of that sort...gonna go rattle snake hunting this summer and hopefully bringing back a bunch of snakes

Extras: Filter Print Post Top
Re: Snake Venom Into Hybrids [Re: dumbfounded1600]
    #7925309 -

Search this forum for snake venom and hybridization.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

Extras: Filter Print Post Top
Re: Snake Venom Into Hybrids [Re: RogerRabbit]
    #7925710 -

What on earth would that do!? You trying to kill someone?


--------------------
You cannot yet touch my daughters awesome boobage
:jiggly:
You must fight to the death in the Breastriary of Nippopolis.




I thought Gene Wilder was cool BEFORE he was dead.

Extras: Filter Print Post Top
Re: Snake Venom Into Hybrids [Re: Funkatron9000]
    #7925732 -

Yes, mycelium cell walls. It helps the nuclei to do whatever it is they do.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

Extras: Filter Print Post Top
Re: Snake Venom Into Hybrids [Re: RogerRabbit]
    #7925780 -

Thats just to crazy! lol, How did someone figure that out ya know...
"Hmmm I think I'll toss some snake venom into this petri here and um... oh crap hybridized myc."

I'm sure there was much more thought in it.... but still.


--------------------
You cannot yet touch my daughters awesome boobage
:jiggly:
You must fight to the death in the Breastriary of Nippopolis.




I thought Gene Wilder was cool BEFORE he was dead.

Extras: Filter Print Post Top
Re: Snake Venom Into Hybrids [Re: Funkatron9000]
    #7927139 -

It breaks down the cell walls in order for them to form

Extras: Filter Print Post Top
Re: Snake Venom Into Hybrids [Re: dumbfounded1600]
    #7927535 -

uhhhh


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread

Extras: Filter Print Post Top
Re: Snake Venom Into Hybrids [Re: tahoe]
    #7928563 -

For real???? Goddamn!

Extras: Filter Print Post Top
Re: Snake Venom Into Hybrids [Re: John Nada]
    #7929101 -

pshhh ..... yeah

Extras: Filter Print Post Top
Re: Snake Venom Into Hybrids [Re: dumbfounded1600]
    #7934024 -

I noticed in the original post that there was refrence to other things being used for this purpose. No one ever mentioned what they were. What are the other additives that could promote that?


--------------------
Our quest for discovery fuels our creativity in all fields, not just science. If we reached the end of the line, the human spirit would shrivel and die. But I don't think we will ever stand still: we shall increase in complexity, if not in depth, and shall always be the center on an expanding horizon of possibilities.
-Stephen Hawking

Extras: Filter Print Post Top
Re: Snake Venom Into Hybrids [Re: mr_minds_eye]
    #7934584 -

I first learned of rattlesnake venom from attending a medicinal mushroom conference in 2005. John Holliday did a presentation on it. Here's a link to a paper he wrote. John Holliday is director of research at alahomedicinals.com and there's lots more info on his website.
RR
http://www.nwbotanicals.org/nwb/lexicon/hybridcordyceps.htm


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

Extras: Filter Print Post Top
Re: Snake Venom Into Hybrids [Re: RogerRabbit]
    #7934642 -

I got a friend who has a ranch and has SHITLOADS of rattles every year...it gets up in the 100s...Im gonna start going up there and milking it..but I dont know how to keep it viable I mean...will it lose its shit being in a air tight container? Can I keep it in the freezer?

Im thinking of doing it and sending it to mycologist for free...I love snakes and amphibians...im also trying to raise Bufo Alvarius Toads and try breeding in captivity without the injection of hormones

Extras: Filter Print Post Top
Re: Snake Venom Into Hybrids [Re: dumbfounded1600]
    #7935739 -

> breeding in captivity without the injection of hormones

Good luck with that. Keep us posted on that in ODD and C&P. I raised one for quite awhile.

I know someone with a bunch of poisonous snakes, so I wonder what kinds of venom would be useful?

I also wonder if the activity is due solely to weakening the cell walls. Chitinase would then work for this also. PEG helps for fusing protoplasts so maybe that would also be a useful additive.


-FF

Extras: Filter Print Post Top
Re: Snake Venom Into Hybrids [Re: fastfred]
    #7935770 -

The venom is not heat stable, so it needs to be added just before pouring the agar, after it's cooled almost to the point of solidifying. For this reason, it must be completely sterile. Sigma Scientific sells purified snake venom, freeze dried into a powder. It has to be stored at -20C or less until use.

I'm not sure of their process, but I've never had it contaminate the agar. It's about three times the price of powder cocaine, so not for everyone who just wants to experiment.

Search this forum for the Redboy thread(s). I had some 20 year old redboy spores that simply would not germinate, no matter what I tried. After placing the non-viable spores on venomated agar for a couple of weeks, I released monokaryotic PR mycelium to crawl over the dish. Low and behold, when the monokaryotic mycelium reached the area where the spores were near the edge, two separate rhizomorphic strains emerged. Somehow the snake venom allowed the PR mycelium to access the nucleus of the spores for mating. I had tried this without the venom to no avail. The very first time venom was used, the result was dikaryotic mycelium, and the redboy strain was back as a 50/50 cross with PR. The experiment was repeated with the offspring, resulting in 75% Redboy(perhaps? I'm not a geneticist), which was then released to the community.
RR

Edited by RogerRabbit (01/26/08 03:42 PM)

Extras: Filter Print Post Top
Re: Snake Venom Into Hybrids [Re: RogerRabbit]
    #7936055 -

Can I get the name to that site?

Interesting story...is the venom expensive?

This process sucks now lol(milking and selling the venom)...I'd have to get a freeze drier because I know now freezer that I have goes below -20...I don't think

Edited by dumbfounded1600 (01/26/08 04:27 PM)

Extras: Filter Print Post Top
Re: Snake Venom Into Hybrids [Re: dumbfounded1600]
    #7939245 -

Not to mention you shouldn't be running around picking up rattlesnakes and grabbing their head to milk them. Your not the crocodile hunter lol. Feel free to post after your hospital experience.


--------------------
My Trade List


I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always worked for me. 
    Hunter S. Thompson



I prefer dangerous freedom over peaceful slavery.
~Thomas Jefferson

Extras: Filter Print Post Top
Re: Snake Venom Into Hybrids [Re: DontPlay]
    #7939316 -

this is very interesting!
RR, have you ever successfully hybridized two strains?

Extras: Filter Print Post Top
Re: Snake Venom Into Hybrids [Re: Knoa6]
    #7939429 -

he just said he did with pr and red boy


--------------------
lol i just lied to whoever was reading that.....

Extras: Filter Print Post Top
Re: Snake Venom Into Hybrids [Re: wikedferret]
    #7939456 -

oh and RR, on that note if you didnt use all of your red boy spores why dont you repeat it with your PR and RB hybrid and make it 75% RB and 25% PR and again until their are only trace amounts or PR to get that RB back? by the 5th try it would be at a 6.25% out of 100 chance that it would resemble a PR that leaves 93.75% RB


--------------------
lol i just lied to whoever was reading that.....

Extras: Filter Print Post Top
Re: Snake Venom Into Hybrids [Re: wikedferret]
    #7939626 -

Quote:
oh and RR, on that note if you didnt use all of your red boy spores why dont you repeat it with your PR and RB hybrid and make it 75% RB and 25% PR


Isn't that exactly what I said I did in my last post? :shrug:

The 75% RB/PR cross used up the last of the original 20 year old spores.  It's all covered in the Redboy thread from four years ago.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3 | 4 | 5 |  [ show all ]

Shop: Original Sensible Seeds High THC Strains   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   Myyco.com Golden Teacher Liquid Culture For Sale   Kraken Kratom Red Vein Kratom   Mushroom-Hut Substrate Bags   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Sporeworks.EU Spores for European Microscopy   PhytoExtractum Maeng Da Thai Kratom Leaf Powder

Mushrooms, Mycology and Psychedelics > Advanced Mycology
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* Snake venom hybridization method debunked (?)
( 1 2 all )		 Pinback 4,752 25 11/19/18 10:11 PM
by Peter65199
* Rattle snake venom dosage Solipsis 663 3 02/23/20 10:25 AM
by murderlabz
* Snake Venom *vs* Chitinase 24sevenZed 495 3 05/05/20 11:00 AM
by 24sevenZed
* Alternative to snake venom in hybridizing.... Justamaninafish 2,514 4 08/21/14 09:01 AM
by Mr. Alien
* questions regarding PE6, rattle snake venom, and home attempts. serotonin storm 924 3 08/28/12 08:09 PM
by weetsie
* Rattle Snake Venom.
( 1 2 all )		 hamloafM 11,447 38 07/13/21 05:53 PM
by glideher
* Snake venom agar tek
( 1 2 all )		 Mycostotle 2,658 37 09/27/20 01:19 PM
by Solipsis
* Snake venom for "stabilization"? Solipsis 465 2 07/22/20 08:24 AM
by Doc9151

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta
24,404 topic views. 0 members, 38 guests and 0 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.032 seconds spending 0.008 seconds on 16 queries.