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bullrider4
Stranger

Registered: 01/13/08
Posts: 6
Last seen: 15 years, 9 months
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Pretty sure I messed up my first attempt.. Confirmation?
#7935156 - 01/26/08 01:36 PM (16 years, 6 days ago) |
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[image]http://www.shroomery.org/forums/files/08-004/137956106-JAR.jpg] [/image]
Hey all,
First time poster here. Been lurking and reading a long time though. Anyway, I inoculated my first batch of jars a week ago today with some spores I got from Spores101. I follwed all the directions to the letter, but a week later, I still have nothing showing in my jars and theres a bit of a funky smell coming from my incubator. Last night, I sacrificed one of the jars to see what was going on. I broke it open and there was no mycelium anywhere- only a funky smell.. Not like a horrible, gross smell, but definitely not a great, earthy smell. I have a theory.. I think that maybe my inoculation holes were too small. Worried about contamination, I only poked two tiny holes in the lids before PC (which I covered with masking tape), and a tinfoil lid. When I inoculated I discovered the holes were too small for the syringe, so I made them just big enough.. But they are very small (see pics).. So do you think my jars arent getting enough air? The incubation temp is 81.. thanks in advance
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highc
creator



Registered: 08/31/07
Posts: 3,592
Loc: maryland
Last seen: 7 years, 2 months
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Re: Pretty sure I messed up my first attempt.. Confirmation? [Re: bullrider4]
#7935355 - 01/26/08 02:18 PM (16 years, 6 days ago) |
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funky smell- yeah probably a bust. The hole size is not a problem i make holes syringe size to. You could use micro-pore tape instead.
what were your sterile conditions???
also if you opened your jar where you inoculate that wasn't a good idea.
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Workman
1999 Spore War Veteran



Registered: 03/01/01
Posts: 3,598
Loc: Oregon, USA
Last seen: 5 hours, 19 minutes
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Re: Pretty sure I messed up my first attempt.. Confirmation? [Re: bullrider4]
#7935381 - 01/26/08 02:24 PM (16 years, 6 days ago) |
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The smell is almost certainly from bacteria. Either the substrate wasn't cooked enough and/or the syringe was contaminated. Hole size isn't an issue and there should have been enough air to at least get the mycelium started. Since you don't seem to be having a mold problem (common airborne contaminate easily killed with heat), your inoculation technique was probably adequate.
Try cooking or sterilizing your substrate longer and try another syringe source. Good luck!
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bullrider4
Stranger

Registered: 01/13/08
Posts: 6
Last seen: 15 years, 9 months
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Re: Pretty sure I messed up my first attempt.. Confirmation? [Re: Workman]
#7935890 - 01/26/08 03:54 PM (16 years, 6 days ago) |
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Quote:
what were your sterile conditions???
also if you opened your jar where you inoculate that wasn't a good idea.
I used a glovebox that I sprayed really well with lysol, and a cigarette lighter that I wiped with alcohol soaked papertowel before inoculation... Never opened the lid. Seemed pretty sterile to me.. It almost looks like the BRF in the jar is kinda slimy.... No idea what happend.. I assume I should have seen growth within a week. anyway, I guess my first attempt was met with failure.. damn the luck.. Try, try another day
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highc
creator



Registered: 08/31/07
Posts: 3,592
Loc: maryland
Last seen: 7 years, 2 months
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Re: Pretty sure I messed up my first attempt.. Confirmation? [Re: bullrider4]
#7935926 - 01/26/08 04:02 PM (16 years, 6 days ago) |
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sounds sterile enough Slimy you say. Like wet looking. How many cc's did you put in.
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