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Pr0_X
CultivationLifer



Registered: 01/18/06
Posts: 617
Loc: Igloo's and polar bears
Last seen: 16 years, 1 day
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Common sense for the average home cultivator (100% sterile print without a professional lab) *DELETED*
#7933600 - 01/26/08 02:53 AM (16 years, 7 days ago) |
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Post deleted by Pr0_XReason for deletion: Why do you guys think? ;)
-------------------- It's okay to hurt my feelings cause you know, they're so numb anyway. but I guess it's what I get for being to fuckin stupid to stay away - Jake - Support the FSR at www.fsrcanada.com and www.fsre.nl
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rizingfire
Mycoticus psychoticus




Registered: 09/10/07
Posts: 875
Loc: North-east USm'f'nA
Last seen: 4 months, 11 days
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Re: Common sense for the average home cultivator (100% sterile print without a professional lab) [Re: Pr0_X]
#7933603 - 01/26/08 02:56 AM (16 years, 7 days ago) |
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I wouldn't say a home lab will be 100% but you can make it close enough tha it doesn't matter. As long as the myc is faster than the mold you are straight
-------------------- aka NHMI
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Pr0_X
CultivationLifer



Registered: 01/18/06
Posts: 617
Loc: Igloo's and polar bears
Last seen: 16 years, 1 day
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Re: Common sense for the average home cultivator (100% sterile print without a professional lab) [Re: rizingfire]
#7933638 - 01/26/08 03:17 AM (16 years, 6 days ago) |
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You don't understand...
That jar you see 75% colonized had a entire print scraped off into it (inside a glove box of course) so if prints are not 100% sterile, why is it clean? it's been incubating for 10 days now, trich would be showing already had the print been not been 100% sterile. especially since the print I used was taken from healthy mycelium that had trich growing fairly close by.
Everyone says "dont do it" i say, try out what I explained. If anyone has any further questions dont be shy pm me and i'll try to explain it abit better. I need some sleep first though so be abit patient
-------------------- It's okay to hurt my feelings cause you know, they're so numb anyway. but I guess it's what I get for being to fuckin stupid to stay away - Jake - Support the FSR at www.fsrcanada.com and www.fsre.nl
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Crasher
αἱρετίζω




Registered: 03/13/01
Posts: 6,220
Loc: Tardy to the Party
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Re: Common sense for the average home cultivator (100% sterile print without a professional lab) [Re: Pr0_X]
#7933651 - 01/26/08 03:24 AM (16 years, 6 days ago) |
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They say don't do it because most of the novice posters on this board don't do enough for sterilization as it is, and can barely get a multispore inoc right, let alone a print directly to a jar.
-------------------- Give me silence, water, hope; Give me struggle, iron, volcanoes...
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Pr0_X
CultivationLifer



Registered: 01/18/06
Posts: 617
Loc: Igloo's and polar bears
Last seen: 16 years, 1 day
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Re: Common sense for the average home cultivator (100% sterile print without a professional lab) [Re: Crasher]
#7933664 - 01/26/08 03:30 AM (16 years, 6 days ago) |
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Well, like I said. If i need to draw a diagram for the new guys I will.
1. Read a glovebox construction tek 2. Grow out a batch 3. Follow the steps ive given for printing 4. Get ready for a supprise
Have faith in yourselfs guys. and dont let anyone ever tell you what you can not do.
-------------------- It's okay to hurt my feelings cause you know, they're so numb anyway. but I guess it's what I get for being to fuckin stupid to stay away - Jake - Support the FSR at www.fsrcanada.com and www.fsre.nl
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DontPlay
Yeah Science!



Registered: 12/13/07
Posts: 750
Loc: On Land most of the time....
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Re: Common sense for the average home cultivator (100% sterile print without a professional lab) [Re: Pr0_X]
#7933835 - 01/26/08 07:08 AM (16 years, 6 days ago) |
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There might only be a few ppl who said it can't be done. The majority of people say it's not recommended. No doing it in a "still air box" at home will not be a 100 percent clean print. Technically when you scrape the spores into a shot glass to make a syringe there not 100 percent clean anymore either but they still work just fine.
-------------------- My Trade List I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always worked for me. Hunter S. Thompson
I prefer dangerous freedom over peaceful slavery. ~Thomas Jefferson
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Pr0_X
CultivationLifer



Registered: 01/18/06
Posts: 617
Loc: Igloo's and polar bears
Last seen: 16 years, 1 day
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Re: Common sense for the average home cultivator (100% sterile print without a professional lab) [Re: DontPlay]
#7933971 - 01/26/08 08:29 AM (16 years, 6 days ago) |
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Actually, it's our own selfishness that causes the failures by trying to maximize something thats already been maximized. It's ignorant of us to think that 100 mushroom caps containing over a million spores each need to be "salvaged" and in a way Crasher is right, "new" guys need to buy a spore syringe and their leading cause of failure is poor sterility of either their enviorment useing to innoc or of the substrate itself but like I said, you need to have success before what im saying can have any effect, if you have success you have the sterility issues under control.
This is a technique that ive recently incorporated since I was having major problems with my own pre-made syringes, preparing your own syringe if your a home cultivator is actually just adding a potentional layer for contamination, if you know the print is sterile well.... straight to substrate it goes, if you have had a succesful grow then you can end up with 12 caps, 12 prints, if printed properlly it's easier to just scrape spores directly into the substrate being used. I did assume people should read my mind so I do appologise.
Listen guys, the theory is right infront of you. I should have took some more pictures to help out the newer guys, im sure the older ones see what im saying about useing peroxcide to insure a 100% print and I did miss out on alot of smaller details... tends to be a problem when im looking at the bigger picture.... So, if a older more experienced cultivator wants to try out what ive mentiond with some excess spawn I know for a fact that I will have your backing and support.
Also, the rye teks for the last three and a half years have not helped me and ive followed them to the T.
Give me some time, i'll go pick up some rye today and do a picture by picture tutorial to explain why some of us just couldnt get the grain thing right.
Just trust me on this 
I understand people are just trying to help each other out, good intentions, good intentions but ya....
I'll write a full tek on this shortly, until then just put the thread on hold. please and thanks.
-------------------- It's okay to hurt my feelings cause you know, they're so numb anyway. but I guess it's what I get for being to fuckin stupid to stay away - Jake - Support the FSR at www.fsrcanada.com and www.fsre.nl
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