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OfflineRogerRabbitM
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Re: Snake Venom Into Hybrids [Re: Ritual]
    #12408870 - 04/17/10 12:50 PM (13 years, 9 months ago)

Do you have a viable culture of this bacteria?
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Invisiblefastfred
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Re: Snake Venom Into Hybrids [Re: RogerRabbit] * 1
    #12409283 - 04/17/10 02:12 PM (13 years, 9 months ago)

And which Vibrio sp. are you talking about?

Obviously you'll not be using V. cholerae.

Rather than trying to get a specific culture it might not be hard to enrich for chitin digesting bacteria.

Just make up some chitin-based agar and throw some organic samples into the mix.  Compost or soil would probably be your best bet.  Then isolate a few species and test them (or their extracts) for how well they dissolve fungal cell walls.

Here's a method for chitin preparation...

Quote:

Chitosan and its precursor, chitin, are typically prepared from waste shells of crustaceans, particularly decapod crustaceans such as crab, shrimp, crawfish, krill, lobster, squid and prawn. The conventional process for producing chitin and chitosan from crustacean shells involves grinding crustacean shells and treating the ground shells with a dilute base (e.g., sodium hydroxide) and heat to remove protein and lipids (deproteinization). Calcium carbonate is removed by extraction with a dilute acid (e.g., hydrochloric acid) at room temperature (demineralization). Following deproteinization and demineralization, the resulting product is predominantly chitin.





-FF


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Offlinedstark
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Re: Snake Venom Into Hybrids [Re: fastfred]
    #12430870 - 04/21/10 09:14 AM (13 years, 9 months ago)

This threat is back to live again =]
The bacteria way sounds more friendly i would say!
I have read abit about the vibrio bacteria, most of them cause problems...


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Offlinealbertz
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Re: Snake Venom Into Hybrids [Re: dumbfounded1600]
    #14038222 - 02/27/11 08:11 PM (12 years, 10 months ago)

there may be product ready to go,.called cobroxin, in many drug stores like wallgreens, made with cobra venom.


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OfflineRogerRabbitM
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Re: Snake Venom Into Hybrids [Re: albertz]
    #14039530 - 02/27/11 11:03 PM (12 years, 10 months ago)

I doubt seriously that cobroxin is nearly toxic enough to work or people couldn't swallow pills of it.  The lab grade product we use is pure venom.  It's also very hard to get.  There's a pretty intensive clearing process and background checks before they'll ship to you.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Offlinealbertz
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Re: Snake Venom Into Hybrids [Re: RogerRabbit]
    #14050801 - 03/01/11 07:59 PM (12 years, 10 months ago)

you do not ingest cobroxin, it is a topical, and it sure could not hurt to experiment with it like in an agar mix or a couple drops on the plate..i will save mine just for that purpose..would be fun to try. It is used to lessen pain and is an anti-inflamatory agent, just out last year and is selling good.


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InvisibleCH HELL
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Re: Snake Venom Into Hybrids [Re: albertz]
    #14051254 - 03/01/11 09:21 PM (12 years, 10 months ago)

albertz are you a cobroxin salesman?  You must be digging up old threads to post about it.  Topical cobroxin is combined with many thing I dont want to put on agar with my spores and surely would'nt help in crossing strains.  I have no clue what pure cobra venom would do but for me that isn't available any way,  but cobroxin wont do shit for mycology.  If it does PLEASE prove me wrong.  If you can't please stop posting in dead threads until you can!

CH


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