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itsallnines
mush-head



Registered: 04/17/07
Posts: 385
Last seen: 10 years, 3 months
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yet another sterile inoculation
#7922436 - 01/23/08 10:54 PM (16 years, 9 days ago) |
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i don't know what to make of it. this is the second batch of cakes that haven't done anything.
PF cakes incubating at about 75F. i used BRF on some and rye flour on the others. the only constant between all of them, and the previous batch, is arrowhead spring water. nothing is growing on them, not even contams. the previous set went for about a month. the current set has about eight days on them.
am i crazy, or is it the water?
-------------------- by their fruits ye shall know them.
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TobesCanaian


Registered: 12/05/07
Posts: 114
Loc:
Last seen: 3 years, 8 months
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Re: yet another sterile inoculation [Re: itsallnines]
#7922451 - 01/23/08 10:56 PM (16 years, 9 days ago) |
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How are you sterlizing them? haha I just use tap water. maybe there is something wrong with your setup? You should give a little more info on what your doing so some people may see some mistakes.
Edited by TobesCanaian (01/23/08 10:57 PM)
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itsallnines
mush-head



Registered: 04/17/07
Posts: 385
Last seen: 10 years, 3 months
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Re: yet another sterile inoculation [Re: itsallnines]
#7922468 - 01/23/08 10:58 PM (16 years, 9 days ago) |
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i used a pressure cooker for about half an hour. like i said, nothing is growing on them, including contams. the last batch, which i inoculated from a spore print, i let go for about a month and nothing grew on them.
-------------------- by their fruits ye shall know them.
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TobesCanaian


Registered: 12/05/07
Posts: 114
Loc:
Last seen: 3 years, 8 months
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Re: yet another sterile inoculation [Re: itsallnines]
#7922504 - 01/23/08 11:05 PM (16 years, 9 days ago) |
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What is your procedure when mixing and putting in jars then innoculating? I know a few things that messed me up when i first did it, ummm sometimes you have to much or to little water that has an effect, i know i injected into the verm layer on top once haha stopped my jar completely, sometimes to packed can mess it up and finally sometimes covering the air holes in the top mess it up, I honestly switched to wbs cause it was much easier for me, they say the pf tek is easier for beginners i beg to differ if you have a pc then you are set to do wbs.
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zyxwvutsr
rstuvwxyz


Registered: 12/30/07
Posts: 141
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Re: yet another sterile inoculation [Re: TobesCanaian]
#7922586 - 01/23/08 11:22 PM (16 years, 9 days ago) |
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incubate about 10 degrees higher guy!
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The shroomy 1
Luminous beings surround me




Registered: 03/27/07
Posts: 5,543
Loc: The Aether
Last seen: 5 months, 5 days
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Re: yet another sterile inoculation [Re: zyxwvutsr]
#7922786 - 01/24/08 12:21 AM (16 years, 9 days ago) |
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There is nothing wrong with your incubation temperatures. Did you say you inoculated with a spore print? Did you mean Spore Syringe?
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AMU Q&A thread.
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thedefone
deus ex machina

Registered: 10/06/07
Posts: 1,883
Loc: Gondwana
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Re: yet another sterile inoculation [Re: zyxwvutsr]
#7922791 - 01/24/08 12:22 AM (16 years, 9 days ago) |
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It's not the water.
Quote:
incubate about 10 degrees higher guy!
Yeah, don't do that. First of all, the cakes themselves will generate a little heat of their own and if you incubate at 85 degrees, not only is that prime contaminate range, but it is also beyond the ideal range of cube mycelium. Not to mention your incubation has next to nothing to do with the spores germinating. My advice is to keep the jars at the temp they are currently at, and to wait another week or so. Where are you getting your spores form?
Quote:
What is your procedure when mixing and putting in jars then innoculating?
Do tell.
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I am become death, the destroyer of worlds.
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itsallnines
mush-head



Registered: 04/17/07
Posts: 385
Last seen: 10 years, 3 months
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Re: yet another sterile inoculation [Re: thedefone]
#7923519 - 01/24/08 09:27 AM (16 years, 8 days ago) |
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i used a spore print to make a spore syringe and used that to inoculate a batch. that was 6 weeks ago. after a month, i had new spore syringes mailed to me and i knocked up a batch with them. same vendor, sporestore.com. a year ago, i got a syringe from them and it worked fine.
the method i used for mixing the cakes, etc., is exactly what i saw on "let's grow mushrooms", with the exception that i used a pressure cooker. then i waited until the next day to inoculate. i am pretty sure that my method was clean as there is NOTHING growing in the jars. no myc, no contams.
if you are interested, this is the reply i got from arrowhead:
Dear X,
Thank you for taking the time to contact us about Arrowhead® Brand Mountain Spring Water. We welcome questions and comments from loyal consumers such as yourself and appreciate this opportunity to assist you.
We have read and researched your inquiry. We do not add any preservatives to our water.
At Arrowhead, we are dedicated to you and your family throughout every phase of your lives. Your feedback is valuable to us, as it helps us to improve our products and services.
We appreciate your interest in our products and hope you will visit our website often for the latest information on our products and promotions.
Sincerely,
Jami Rizzo Consumer Response Representative
-------------------- by their fruits ye shall know them.
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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Re: yet another sterile inoculation [Re: itsallnines]
#7923526 - 01/24/08 09:31 AM (16 years, 8 days ago) |
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its for sure not the water... you either got bad syringes or prints (twice in a row?) or you have too little/too much water in the jars... unless you have the jars all the way sealed with no holes for gas exchange...
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itsallnines
mush-head



Registered: 04/17/07
Posts: 385
Last seen: 10 years, 3 months
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Re: yet another sterile inoculation [Re: DJYoshaBYD]
#7923563 - 01/24/08 09:45 AM (16 years, 8 days ago) |
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i am pretty sure that the water mix is at least close. i do have foil over the tops of the jars, but loose. it is in no way sealed.
-------------------- by their fruits ye shall know them.
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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Re: yet another sterile inoculation [Re: itsallnines]
#7923596 - 01/24/08 09:56 AM (16 years, 8 days ago) |
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well.. it sounds like your brown rice flour has fungicide in it... that actually halted my grow before... once... you said NOTHING grows in it, not even contams? even after 6 weeks? sounds like fungicide to me... try a different brand of brown rice...
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itsallnines
mush-head



Registered: 04/17/07
Posts: 385
Last seen: 10 years, 3 months
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Re: yet another sterile inoculation [Re: DJYoshaBYD]
#7923639 - 01/24/08 10:12 AM (16 years, 8 days ago) |
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for some of the cakes i used organic rye. the others, i used organic brown rice flour.
the cakes i did over a month ago, i used lundbergh(?) eco friendly (or whatever the bag says) brown rice that i turned into flour in the blender.
-------------------- by their fruits ye shall know them.
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itsallnines
mush-head



Registered: 04/17/07
Posts: 385
Last seen: 10 years, 3 months
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Re: yet another sterile inoculation [Re: itsallnines]
#7923652 - 01/24/08 10:20 AM (16 years, 8 days ago) |
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after re-reading the PF-tek, i am wondering if the jars are getting dried out from the heat of the PC and being removed too soon. usually, i PC them for about half an hour, release the pressure and remove the cakes. i am thinking that perhaps removing them while they are still so hot is causing the water within to steam off.
-------------------- by their fruits ye shall know them.
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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Re: yet another sterile inoculation [Re: itsallnines]
#7923669 - 01/24/08 10:24 AM (16 years, 8 days ago) |
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thats odd.. well it has to be one of the above... soooo
water too high or too low.... you said you followed the tek exactly
bad spore syringe or print.... you said you tried different syringes different times...
gas exchange... loosely covered with foil...
brown rice or rye has fungicide... you said its organic (just because it says that, doesnt mean there is no fungicide, even though it should.)
take you pick... that is what will cause your problem, so you have to start troubleshooting a lil bit.. first, go over the preparation of the jars again, and make sure you are doing everything right.. then, try a different brand of brown rice from a different store... then, if all else fails, get another syringe from ANOTHER dealer, like ralphster's or sporeworks, and try that... other than that, I dont know what else to tell you...
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itsallnines
mush-head



Registered: 04/17/07
Posts: 385
Last seen: 10 years, 3 months
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Re: yet another sterile inoculation [Re: DJYoshaBYD]
#7923691 - 01/24/08 10:30 AM (16 years, 8 days ago) |
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exactly. i am completely stumped.
in the past, i used regular tap water and it worked fine. i used the same brand of rice and it worked fine. i steamed instead of PC'ed and it worked, but i lost a couple jars.
i am going to wait two more days (its been nine days since inoculation) and open one of the jars to see if i smell anything odd and to examine the moisture content.
-------------------- by their fruits ye shall know them.
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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Re: yet another sterile inoculation [Re: DJYoshaBYD]
#7923694 - 01/24/08 10:32 AM (16 years, 8 days ago) |
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no.. you would still have moisture in there to at least get them to colonize a lil bit...
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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Re: yet another sterile inoculation [Re: DJYoshaBYD]
#7923703 - 01/24/08 10:34 AM (16 years, 8 days ago) |
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well.. dont mess with them yet.. spores take a bit to germinate... give them another 5 days in the dark, without looking or exposing them to light AT ALL... if you dont see any growth, then try it a different way... when I noc up jars from syringe, even keeping them at a perfect incubation temp, its till takes 10 to 15 days to see ANY growth... but then they just take off and fully colonize in about a week or so...
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itsallnines
mush-head



Registered: 04/17/07
Posts: 385
Last seen: 10 years, 3 months
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Re: yet another sterile inoculation [Re: DJYoshaBYD]
#7923707 - 01/24/08 10:35 AM (16 years, 8 days ago) |
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ok, now i am REALLY stumped.
maybe i'll get new syringes and try the WBS tek. i was going to case these cakes anyway.
-------------------- by their fruits ye shall know them.
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itsallnines
mush-head



Registered: 04/17/07
Posts: 385
Last seen: 10 years, 3 months
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Re: yet another sterile inoculation [Re: DJYoshaBYD]
#7923711 - 01/24/08 10:36 AM (16 years, 8 days ago) |
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Quote:
DJYoshaBYD said: well.. dont mess with them yet.. spores take a bit to germinate... give them another 5 days in the dark, without looking or exposing them to light AT ALL... if you dont see any growth, then try it a different way... when I noc up jars from syringe, even keeping them at a perfect incubation temp, its till takes 10 to 15 days to see ANY growth... but then they just take off and fully colonize in about a week or so...
maybe i am just antsy, being that the last batch failed to do anything for a month.
-------------------- by their fruits ye shall know them.
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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Re: yet another sterile inoculation [Re: itsallnines]
#7923752 - 01/24/08 10:51 AM (16 years, 8 days ago) |
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shit.. tell me about it... I lost 8 monotubs with hpoo when I first tried it, when I was 16, because I got impatient and fucked with them and recased and opened them... etc etc etc...
just let them sit another 5 days in the dark without looking... other than that, another tek might be good.. wbs is good, just alot of prep.. there have been quite a few cats on here who just skipped (or tried and failed) the pf tek and went straight to wbs and others, and had more success... just keep trying.. you will get it down...
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